Results 151 to 160 of about 39,685 (269)
Construction of plasmids for gp64-VP1 expression by baculovirus.
, 2013 The VP1 gene was incorporated between gp64 signal peptide and gp64 mature domain by overlapping PCR. The recombinant gene gp64-VP1 was then inserted into pFastBac HT A. The resultant plasmids were designated pFast-Pph-gp64-VP1.
Jimmy Kwang (61087) +4 more
core +1 more source
The characteristics and genomic analysis of Vibrio cholerae phage VP1
VirologyThe lytic bacteriophage VP1 specifically targets Vibrio cholerae (V. cholerae), showing excellent therapeutic potential. It features a 55-min latency period, large burst size (255 virions/cell), and remarkable stability (pH 5-12). Genomic sequencing revealed a double-stranded DNA genome of 42,845 bp with a G + C content of 45.49 %.
Xi Chen +4 more
openaire +2 more sources
Time course of expression of MCPyV-VP1.
, 2015 Insect Tn5 cells were infected with AcMCPyV-VP1 and harvested for the indicated time periods. Five microliters of culture medium and samples of lysate from 105 cells were analyzed by SDS-PAGE. Protein bands were visualized by Coomassie blue staining (CB;
Michiyo Kataoka (485324) +9 more
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Construction of chimeric mouse polyomavirus surface vp1 proteins exhibiting hcv epitopes.
, 2010 In the present study, plasmids for expression of major capsid proteins VP1 of murine polyomavirus with inserted sequences from Hepatitis C virus envelope proteins in yeast S. cerevisiae were constructed.
Sabaliauskaitė, Rasa,
core
鸭肝炎病毒鸡胚化弱毒MY株VP1基因克隆、原核表达及抗原性分析
, 2010 应用巢氏PCR(nested-PCR)扩增出1型鸭肝炎病毒鸡胚化弱毒MY株结构蛋白VP1基因片段,将VP1克隆到pMD18-T载体上,测序结果为714 bp,GenBank登录号:GU363950。对MY株VP1基因编码蛋白的主要抗原位点进行预测,aa208-aa222氨基酸区段表现很高的亲水性、抗原指数和表面可及性。VP1经EcoRⅠ和XhoⅠ双酶切后克隆至pET32a(+)原核表达载体,获得重组质粒pET-VP1,转入BL21 PLyss(DE3)细胞中,IPTG诱导后SDS-PAGE检测结果表明 ...
刘群 +5 more
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“yibian VP1 yibian VP2”VS“VP1 de shihou VP2”
The Journal of Study on Language and Culture of Korea and China, 2014 openaire +1 more source
Development of VP1 based indirect ELISAs for BK and JC polyomaviruses with seroprevalence assessment and cross reactivity evaluation. [PDF]
Sci RepAlipour AH, Fallah FH, Kiasari BA.
europepmc +1 more source
A chimeric trivalent Echovirus vaccine designed by loop substitution elicits cross-neutralizing immunity against serotypes 11, 18, and 30. [PDF]
J VirolJiang S +10 more
europepmc +1 more source
Development of canine parvovirus-2-based recombinant pseudoviruses expression system: a potential vaccine platform. [PDF]
Vet ResMiao B +9 more
europepmc +1 more source