Results 61 to 70 of about 49,884 (215)

Identification Epitope on VP4 Protein of Senecavirus A Recognized by a Monoclonal Antibody

Animal Research and One Health, EarlyView.
This study aims to identify conserved antigenic epitopes on the SVA VP4 protein using monoclonal antibodies, providing valuable tools for further exploring its functions and developing detection methods. SVA VP4 protein was expressed in Escherichia coli and purified with High‐Affinity Ni‐Charged Resin FF.
Liang Meng, Xuyan Xiang, Xinyu Qi, Hanrong Zhou, Peiyu Xiao, Fandan Meng, Tongqing An, Haiwei Wang   +7 more
wiley   +1 more source

Construction of cell line stably expressing IBDV VP 2 protein mutants

浙江大学学报. 农业与生命科学版, 2011
A couple of primers was designed on the base of VP2 gene sequence of infectious bursal disease virus (IBDV) HZ2 strain, and the VP2 gene was successfully cloned into eukaryotic expression vector pDsRed2-N1.
SUN Jing, WEI Yong-wei, ZHANG Xiao-dong, YU Lian   +3 more
doaj   +1 more source

Western blot analysis to confirm VP2 expression.

, 2015
Chicken embryo fibroblast cells were infected with recombinant baculoviruses and cell lysates were used in Western blot analysis to detect VP2 protein expression. (A) BV-S-ITRs-VP2; (B) BV-S-ITRs-VP2/4/3; (C) β-actin control.
Qi An (331241), Jingping Ge (636557), Wenxiang Ping (636561), Shanshan Song (500562), Dongni Gao (769100)   +4 more
core   +1 more source

Duck Orbivirus and Egg Production: A Newly Identified Duck Orbivirus Is the Etiological Agent of Egg Production Decline in Chinese Breeder Ducks

Animal Research and One Health, EarlyView.
Field samples from breeder duck farms with egg‐production decline were subjected to viral detection, genome sequencing, and virus isolation. Experimental infection demonstrated that duck orbivirus (DORV) causes a significant and persistent reduction in egg production in breeder ducks.
Bing Li, Jianhua Wang, Xuezhi Cui, Xinxing Chu, You Wang, Dongmin Hao, Mingqing Xu, Haixia Chang, Huihui Li, Mingtian Mao, Mian Wu, Chengguang Lu, Jing Tang, Suyun Liang, Zhanbao Guo, Zhengkui Zhou, Youxiang Diao, Shuisheng Hou, Yi Tang   +18 more
wiley   +1 more source

Three-dimensional structural analysis of recombinant rotavirus-like particles with intact and amino-terminal-deleted VP2: implications for the architecture of the VP2 capsid layer [PDF]

Journal of Virology, 1997
Rotaviruses are the leading cause of severe infantile gastroenteritis worldwide. These viruses are large, complex icosahedral particles consisting of three concentric capsid layers enclosing a genome of eleven segments of double-stranded RNA (dsRNA). The amino terminus of the innermost capsid protein VP2 possesses a nonspecific single-stranded RNA and ...
Lawton, J.A., Zeng, C.Q.Y., Mukherjee, S.K., Cohen, J., Estes, M.K., Prasad, B.V.V.   +5 more
openaire   +3 more sources

Display of multiple proteins on engineered canine parvovirus-like particles expressed in cultured silkworm cells and silkworm larvae

Frontiers in Bioengineering and Biotechnology, 2023
Recent progress has been made dramatically in decorating virus-like particles (VLPs) on the surface or inside with functional molecules, such as antigens or nucleic acids.
Jian Xu, Tomofumi Sekiguchi, Jirayu Boonyakida, Tatsuya Kato, Tatsuya Kato, Tatsuya Kato, Enoch Y. Park, Enoch Y. Park, Enoch Y. Park   +8 more
doaj   +1 more source

What Makes an “Ideal” Cell Line for Recombinant Adeno‐Associated Virus Production?

Biotechnology and Bioengineering, EarlyView.
Several host cell types have been used to produce rAAVs to date. Cell line‐specific traits that are beneficial in the context of rAAV manufacturing are reviewed here, with the goal of developing a consensus on the ‘ideal’ characteristics that an rAAV production host should possess (created with Biorender.com).
James Conheady, Nicholas Donohue, Alexandra Bogdanovic, Sharon Davin, Brian Glennon, Niall Barron, C. Mark Smales   +6 more
wiley   +1 more source

Protection from infectious bursal disease virus (IBDV)-induced immunosuppression by immunization with a fowlpox recombinant containing IBDV-VP2

, 2010
Immunosuppression resulting from infectious bursal disease virus (IBDV) infection has critical health and welfare implications for birds, yet it is incompletely understood and largely overlooked as a measure of vaccine efficacy.
T. F. Davison (17195419), B. J. G. Baaten (17195410), Colin Butter (17154925), T. D. M. Sturman (17195446)   +3 more
core   +1 more source

Nuclear localization of MCV VP2 expressed +/− VP1.

, 2013
Confocal immunofluorescent microscopy of 293TT cells transfected with VP1 (red), VP2 (green) or VP1 and VP2 from a bicistronic plasmid. A mouse monoclonal antibody was used to detect VP1, and a rabbit polyclonal antibody was used to detect VP2 ...
Christopher B. Buck (121726), Rachel M. Schowalter (121719)   +1 more
core   +1 more source

Ion Activation Methods for Top‐Down Proteomics

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley   +1 more source