Results 81 to 90 of about 49,884 (215)

Expanding the Fish‐Brain Invitrome With the Senegalese Sole SsB‐1 Cell Line—A Versatile Model for Neurotropic Virus Research

Journal of Fish Diseases, EarlyView.
ABSTRACT Cell lines are essential tools for studying animal physiology and immunopathology, reducing the use of live animals and supporting the 3Rs principle of reduction, refinement, and replacement. The Senegalese sole (Solea senegalensis), a relevant species for Spanish aquaculture diversification, lacks established in vitro models. We developed SsB‐
Yulema Valero, Alberto Cuesta
wiley   +1 more source

Capture of adeno‐associated viruses from clarified lysate by continuous flow ultracentrifugation: a comparative study of iodixanol and sucrose gradients

Journal of Chemical Technology &Biotechnology, Volume 101, Issue 7, Page 1384-1395, July 2026.
Abstract BACKGROUND Adeno‐Associated Virus vectors are widely used in gene therapy due to their safety and efficacy. Despite the interest in their therapeutical application, AAV purification remains a significant challenge. Density gradient ultracentrifugation using cesium chloride, sucrose, or iodixanol gradients are common methods for AAV ...
Sabrina Leigheb, Maral Rahimzadeh, Nikolaus Virgolini, Aida Tleuova, Mahbod Mousavian, Astrid Dürauer, Patricia Pereira Aguilar   +6 more
wiley   +1 more source

Construction and efficacy of recombinant Newcastle disease virus co-expressing VP2 and VP3 proteins of very virulent infectious bursal disease virus

Poultry Science
Infectious bursal disease (IBD), triggered by the infectious bursal disease virus (IBDV), poses a substantial risk to the poultry industry due to its immunosuppressive nature and the emergence of highly virulent strains.
Tongfei Liu, Lin Lin, Yun Pan, Xiaoling Lin, Ming Liang, Guanming Shao, Keyu Feng, Yaxin Liu, Xinheng Zhang, Qingmei Xie   +9 more
doaj   +1 more source

Fatal Myocarditis Caused by Canine Parvovirus in a Litter of One‐Day‐Old German Shepherd Puppies: Molecular and Histopathological Findings

Veterinary Medicine and Science, Volume 12, Issue 4, July 2026.
Canine parvovirus infection resulted in acute fatal myocarditis in one‐day‐old German Shepherd puppies. Histopathology demonstrated lymphoplasmacytic myocarditis with intranuclear inclusion bodies, and PCR confirmed viral DNA in cardiac tissue. These findings indicate perinatal transmission and emphasise that CPV myocarditis remains a lethal threat to ...
Seyedhosein Jarolmasjed, Mohammadreza Ghorani, Amir Ali Shahbazfar, Hamed Sepehr, Seyed Hossein Zamzam, Sina Soleimani   +5 more
wiley   +1 more source

Chaperonin TRiC/CCT subunit CCT7 is involved in the replication of canine parvovirus in F81 cells

Frontiers in Microbiology
Canine parvovirus (CPV) is one of the most common lethal viruses in canines. The virus disease is prevalent throughout the year, with high morbidity and mortality rate, causing serious harm to dogs and the dog industry.
Xia Su, Hongzhuan Zhou, Fuzhou Xu, Jin Zhang, Bing Xiao, Qi Qi, Lulu Lin, Bing Yang   +7 more
doaj   +1 more source

VP2_121-130 mutants.

, 2014
VP2121-130 mutants.
Danielle N. Renner (354030), Kevin D. Pavelko (331636), Aaron J. Johnson (330991), Michael P. Bell (331640)   +3 more
core   +1 more source

A Mechanistic Model of rAAV Production in Synthetic Cell Lines

Biotechnology and Bioengineering, Volume 123, Issue 6, Page 1684-1694, June 2026.
ABSTRACT The recombinant adeno‐associated virus (rAAV) is a widely used vector for gene therapy. Its manufacturing faces significant challenges in producing the large quantities of vectors needed for clinical applications and reducing empty particles.
Prahalad Srinivasan, Han‐Jung Kuo, Yu‐Chieh Lin, Yen‐An Lu, Wei‐Shou Hu, Qi Zhang   +5 more
wiley   +1 more source

Development of TMEV VP2_121-130 mutant viruses.

, 2014
(A) Real-time RT-PCR expression analysis of viral VP2 and plasmid neomycin phosphotransferase from BHK cells transfected with TMEV VP2121-130 mutant cDNA. (B) Western blot analysis of whole cell lysates and viral supernatants for TMEV viral proteins from
Danielle N. Renner (354030), Kevin D. Pavelko (331636), Aaron J. Johnson (330991), Michael P. Bell (331640)   +3 more
core   +1 more source

Applying Distinct CDMS Strategies to Observe Nonclassical Virus Capsid Assembly

Journal of Mass Spectrometry, Volume 61, Issue 6, June 2026.
ABSTRACT In conventional native mass spectrometry (MS), one faces severe limitations when challenged with heterogeneous, high‐mass samples, commonly failing to resolve clear peak distributions, and thus mass determination. Charge detection MS (CDMS) has emerged as a premier method to analyze these samples by determining mass‐to‐charge ratio (m/z) and ...
Lars Thiede, Anisha Haris, Tomislav Damjanović, Jocky C. K. Kung, Jürgen Müller‐Guhl, Ronja Pogan, Jennifer Rothe, Wiebke Schultze, Sanne Ugelstad, David Eatough, Kevin Giles, Steve Preece, Keith Richardson, Jakub Ujma, Charlotte Uetrecht   +14 more
wiley   +1 more source

Topographical analysis of canine parvovirus virions and recombinant VP2 capsids

Journal of General Virology, 1993
The distribution of epitopes defined by monoclonal antibodies (MAbs) on the surface of canine parvovirus (CPV) virions and recombinant VP2-capsids was established using immunoelectron microscopy. A correlation appeared to exist between the linear position, neutralizing activity and immunogold staining.
E, Cortes, C, San Martin, J, Langeveld, R, Meloen, K, Dalsgaard, C, Vela, I, Casal   +6 more
openaire   +2 more sources