Results 41 to 50 of about 52,591 (172)
Expression and purification of CPV-VP2 proteins.
Protein detection using SDS-PAGE (a): lane 1, His-Sumo-VP2 (85 kDa); lane 2, VP2 protein (65 kDa). Identification of proteins using Western blot (b): lane 1, His-Sumo-VP2; lane 2, VP2 protein.
Ye Jin (286793) +7 more
core +1 more source
ABSTRACT Background Cholangiolocarcinoma (CLC) is a rare liver cancer with ≥ 80% ductular configuration (DC). Given its rarity, its clinicopathological characteristics remain poorly understood, and its status as a distinct disease entity remains controversial.
Takamichi Ishii +22 more
wiley +1 more source
Construction of cell line stably expressing IBDV VP 2 protein mutants
A couple of primers was designed on the base of VP2 gene sequence of infectious bursal disease virus (IBDV) HZ2 strain, and the VP2 gene was successfully cloned into eukaryotic expression vector pDsRed2-N1.
SUN Jing +3 more
doaj +1 more source
Immunogenicity of VLP-VP2 in mice (determination of antibody titers of VLP-VP2 in mice).
Immunogenicity of VLP-VP2 in mice (determination of antibody titers of VLP-VP2 in mice).
Yu-wei Gao (510434) +13 more
core +1 more source
Ion Activation Methods for Top‐Down Proteomics
ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley +1 more source
Preparation and immunogenicity studies of NvIBDV VP2-ferritin nanoparticles
Background Infectious bursal disease (IBD), caused by infectious bursal disease virus (IBDV), is a highly contagious disease that is prevalent worldwide and poses a significant threat to the poultry industry.
Shimin Gao +14 more
doaj +1 more source
Recent progress has been made dramatically in decorating virus-like particles (VLPs) on the surface or inside with functional molecules, such as antigens or nucleic acids.
Jian Xu +8 more
doaj +1 more source
Effect of VP2 myristoylation on transduction.
(A) Purified MCV pseudoviruses made with WT VP2 or Gly2-mutated VP2 (G2F, G2S, and G2V) were analyzed by SDS-PAGE and SYPRO Ruby protein stain. (B) The infectivity of MCV pseudoviruses containing WT VP2 or Gly2-mutated VP2 was determined in 293TT cells ...
Christopher B. Buck (121726) +1 more
core +1 more source
Enterovirus infections in children
Enteroviruses (EVs) are major pathogens causing a wide spectrum of diseases in children worldwide. This review summarizes the epidemiology, virology, viral replication, and virus‐host interactions of EVs. It also highlights current advances in clinical diagnosis, antiviral therapies, and vaccine development, providing insights into future strategies ...
Xiangpeng Chen +11 more
wiley +1 more source
Western blot analysis to confirm VP2 expression.
Chicken embryo fibroblast cells were infected with recombinant baculoviruses and cell lysates were used in Western blot analysis to detect VP2 protein expression. (A) BV-S-ITRs-VP2; (B) BV-S-ITRs-VP2/4/3; (C) β-actin control.
Qi An (331241) +4 more
core +1 more source

