Establishment of Sandwich ELISA for Quality Control in Rotavirus Vaccine Production
Vaccines, 2022 Non-replicating rotavirus vaccines are alternative strategies that may improve the protective efficacy of rotavirus vaccines in low- and middle-income countries.
Cao Li +9 more
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Viruses, 2021 The bluetongue virus (BTV) is transmitted by Culicoides biting midges and causes bluetongue (BT), an OIE-notifiable disease of ruminants. At least 29 BTV serotypes are described as determined by the outer shell proteins VP2 and VP5.
Piet A. van Rijn +2 more
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Proteolysis of Monomeric Recombinant Rotavirus VP4 Yields an Oligomeric VP5* Core [PDF]
Journal of Virology, 2001 ABSTRACT Rotavirus particles are activated for cell entry by trypsin cleavage of the outer capsid spike protein, VP4, into a hemagglutinin, VP8*, and a membrane penetration protein, VP5*. We have purified rhesus rotavirus VP4, expressed in baculovirus-infected insect cells.
Dormitzer, Philip R. +2 more
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Infectious bursal disease virus VP5 polypeptide: a phosphoinositide-binding protein required for efficient cell-to-cell virus dissemination. [PDF]
PLoS ONE, 2015 Infectious bursal disease virus (IBDV), a member of the Birnaviridae family, is a major avian pathogen responsible for an immunosuppressive disease affecting juvenile chickens. The IBDV genome is formed by two dsRNA segments.
Fernando Méndez +3 more
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A 3D model of the membrane protein complex formed by the white spot syndrome virus structural proteins. [PDF]
PLoS ONE, 2010 BACKGROUND: Outbreaks of white spot disease have had a large negative economic impact on cultured shrimp worldwide. However, the pathogenesis of the causative virus, WSSV (whit spot syndrome virus), is not yet well understood.
Yun-Shiang Chang +11 more
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The Kinetics of VP5 mRNA Expression Is Not Critical for Viral Replication in Cultured Cells [PDF]
Journal of Virology, 2000 ABSTRACTWe generated recombinant viruses in which the kinetics of expression of the leaky-late VP5 mRNA was altered. We then analyzed the effect of such alterations on viral replication in cultured cells. The VP5 promoter and leader sequences from positions −36 to +20, containing the TATA box and an initiator element, were deleted and replaced with a ...
P T, Lieu, E K, Wagner
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The rotavirus VP5*/VP8* conformational transition permeabilizes membranes to Ca 2+
, 2023 ABSTRACT Rotaviruses infect cells by delivering into the cytosol a transcriptionally active inner capsid particle (a "double-layer particle": DLP). Delivery is the function of a third, outer layer, which drives uptake from the cell surface into small vesicles from which the DLPs escape.
Marilina De Sautu +3 more
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Genetic and phylogenetic analysis of the outer capsid protein genes of Indian isolates of bluetongue virus serotype-16 [PDF]
Veterinary World, 2018 Aim: The aim of the study was to characterize bluetongue virus serotype 16 (BTV-16), recently isolated from different states of India. The evolutionary relationship of newly isolated BTV-16 and previously reported Indian and global BTV-16 isolates were ...
Arpit Saxena +9 more
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Alternative intermolecular contacts underlie the rotavirus VP5* two‐ to three‐fold rearrangement [PDF]
The EMBO Journal, 2006 The spike protein VP4 is a key component of the membrane penetration apparatus of rotavirus, a nonenveloped virus that causes childhood gastroenteritis. Trypsin cleavage of VP4 produces a fragment, VP5*, with a potential membrane interaction region, and primes rotavirus for cell entry.
Joshua D, Yoder, Philip R, Dormitzer
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Primed for Discovery: Atomic-Resolution Cryo-EM Structure of a Reovirus Entry Intermediate
Viruses, 2010 A recently solved structure of the aquareovirus virion (Zhang, X; Jin, L.; Fang, Q; Hui, W.H.; Zhou Z.H. 3.3 Å Cryo-EM Structure of a Nonenveloped Virus Reveals a Priming Mechanism for Cell Entry.
Shane D. Trask +2 more
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