Use of VP60 RT-PCR to overcome the limitation of haemagglutination inhibition diagnosis of rabbit viral haemorrhagic disease [PDF]
This work was partially funded by the Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt, and by Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Shakal, M.A. +4 more
openaire +4 more sources
Background Prior to 2010, the lagoviruses that cause rabbit hemorrhagic disease (RHD) in European rabbits (Oryctolagus cuniculus) and European brown hare syndrome (EBHS) in hares (Lepus spp.) were generally genus-specific.
Aleksija S. Neimanis +7 more
doaj +2 more sources
Serological characterisation of Lagovirus virus-like particles originating from native and mutated VP60 of rabbit haemorrhagic disease virus 2 and European brown hare syndrome virus [PDF]
Since lagoviruses cannot be cultivated in vitro, using expression systems is an alternative and promising way of producing diagnostic viral antigens. It opens up their use as active immunogens for vaccine production.
Krejmer-Rąbalska Martyna +3 more
doaj +2 more sources
Recombination between non-structural and structural genes as a mechanism of selection in lagoviruses: The evolutionary dead-end of an RHDV2 isolated from European hare [PDF]
The genus Lagovirus, belonging to the family Caliciviridae, emerged around the 1980s. It includes highly pathogenic species, rabbit hemorrhagic disease virus (RHDV/GI.1) and European brown hare syndrome virus (EBHSV/GII.1), which cause fatal hepatitis ...
Patrizia Cavadini +4 more
doaj +2 more sources
Detection and Characterisation of The 'A' Variant of Rabbit Haemorrhagic Disease (RHDVa, GI.1a) Over a Decade (2013-2023) in Italy. [PDF]
Since the 1980s, highly pathogenic and hepatotropic Lagovirus species have significantly impacted rabbit populations, including Rabbit Haemorrhagic Disease Virus (RHDV, genotype GI.1), its variant RHDVa (genotype GI.1a), and RHDV type 2 (RHDV2, genotype GI.2).
Di Giovanni V +4 more
europepmc +2 more sources
Nicotiana benthamiana as a Source of Cowpea Mosaic Virus-Derived Particles That Specifically Package Designer RNAs. [PDF]
ABSTRACT The coupling of replication and encapsidation in the bipartite plant virus, cowpea mosaic virus (CPMV), has been used to specifically incorporate designer RNA molecules into viral particles via transient expression in Nicotiana benthamiana. This is achieved by placing the desired RNA sequence between the 5′ and 3′ UTRs of CPMV RNA‐2 so that ...
Peyret H +5 more
europepmc +2 more sources
Novel Recombinant Rabbit Hemorrhagic Disease Virus 2 (RHDV2) is Circulating in China within 12 Months after Original RHDV2 Arrival. [PDF]
Rabbit hemorrhagic disease (RHD) causes lethal fulminant hepatitis in rabbits. Two different genotypes (GI.1 and GI.2) responsible for RHD are reported. GI.2 was first detected in France in 2010 and subsequently spread to other countries in Europe. In April 2020, GI.2 was detected in China.
Hu B +9 more
europepmc +2 more sources
Specific Detection of RHDV GI.1 and GI.2 by RT-LAMP-CRISPR/Cas12a Platform. [PDF]
Rabbit hemorrhagic disease is a highly contagious and acute fatal disease caused by rabbit hemorrhagic disease virus (RHDV). The first outbreak of RHDV2 in 2020 has posed a serious threat to the rabbit breeding industry in China. An effective and specific detection strategy for RHDV GI.1 (RHDV1) and GI.2 (RHDV2) is urgently needed.
Wu M +9 more
europepmc +2 more sources
Nisin-controlled expression of Norwalk virus VP60 protein in Lactobacillus casei [PDF]
A food-grade strain with nisRK stably integrated into the genome, was constructed in order to implement the nisin-controlled expression system (NICE) in Lactobacillus casei ATCC393. Expression of β-glucuronidase (gus) reporter gene was employed to optimize the system, which has been successfully used to produce the main antigenic protein from Norwalk ...
M. Cruz MartÃn +5 more
openaire +2 more sources
Phylogenetic Analysis of European Brown Hare Syndrome Virus Strains from Poland (1992–2004)
European brown hare syndrome (EBHS) is lethal to several species of free-living hares worldwide. The genetic characterization of its virus (EBHSV) strains in European circulation and epidemiological knowledge of EBHSV infections is not yet complete.
Andrzej Fitzner +5 more
doaj +1 more source

