Results 131 to 140 of about 26,217 (212)
Recombination analysis of Tat exon 1 and Vpr variants.
, 2013 Sequences of Tat 71, Vpr 45 and Vpr 46 were analyzed by Sim Plot. They were aligned with HIV-1 subtype B, C and D consensus sequences and subjected to the boot scan analysis. Tat 71 was turned out to be a recombinant of subtype B and C. Vpr 45 and Vpr 46
Vishnampettai G. Ramachandran (499654) +6 more
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Expression and stability of Vpr natural variants.
, 2013 (a) 293 T cells were transfected with myc fusion constructs of wild type B Vpr, C Vpr and Vpr variants (2 µg each) using Lipofectamine 2000; the cell lysates were prepared 24 hrs post-transfection and were run on 12 percent SDS PAGE. Western blotting was
Vishnampettai G. Ramachandran (499654) +6 more
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Circulating Vpr under HIV viremic conditions and oncovirus-related malignancies.
(A) Stacked bar chart showing the proportion of Vpr-positive samples in HIV patients with aviremia (Av) and viremia (V). Black bar, Vpr positivity. White bar, Vpr negativity. Violin plot showing the distribution of Vpr concentrations (ng/mL). Dotted line
Akihiro Matsunaga (3449834) +6 more
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, 2011 Copyright information:Taken from "Localization of HIV-1 Vpr to the nuclear envelope: Impact on Vpr functions and virus replication in macrophages"http://www.retrovirology.com/content/4/1/84Retrovirology 2007;4():84-84.Published online 26 Nov 2007PMCID ...
Erwann Le Rouzic (77460) +8 more
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Purification of recombinant Vpr protein and binding analysis by SPR.
, 2016 (a) SDS-PAGE analysis of purified recombinant proteins. COS-7 cells were transfected with mammalian pCAGGS vectors encoding FLAG-mRFP (mRFP) or FLAG-mRFP-Vpr (mRFP-Vpr) and then purified on ANTI-FLAG M2 agarose beads.
Shin-nosuke Takeshima (623367) +12 more
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Vpr influences the expression level of GRP78 and Mfn2 proteins.
, 2013 A, At 48 hours post-transfection, expression of Vpr-GFP or Vpr52–96-GFP up-regulated GRP78 level, but reduced Mfn2 level in HEK293 cells. B, Vpr-related decrease in the expression of Mfn2 was time-dependent after Lenti-Vpr infection.
Shiow-Her Chiou (329314) +4 more
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Essential CSR factors interact with Vpr.
, 2013 (a) Schematics of the principle of the BiFC assays to analyze interaction of 14-3-3ζ (Flag–14-3-3ζ–EYFP1–154), AID (Flag–AID–EYFP1–154), PKA-Cα (Flag–PKA-Cα–EYFP1–154) or Ung (Flag–Ung–EYFP1–154) with Vpr (HA–Vpr–EYFP155–238).
Tonika Lam (489334) +6 more
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, 2011 Copyright information:Taken from "Localization of HIV-1 Vpr to the nuclear envelope: Impact on Vpr functions and virus replication in macrophages"http://www.retrovirology.com/content/4/1/84Retrovirology 2007;4():84-84.Published online 26 Nov 2007PMCID ...
Erwann Le Rouzic (77460) +8 more
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Functional Analysis of Vpr Mutants
, 2013 (A) SupT1 cells were infected with pHR-VPR-R or indicated mutants, at an MOI of 0.5. At 48 h postinfection, cells were stained with hypotonic PI to determine the cell cycle profiles.
Orly Ardon (31765) +7 more
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Differential Binding of Host Cell Proteins by Vpr Mutant R77Q Compared with Wild Type Vpr [PDF]
HIV Viral protein R (Vpr) is a multifunctional accessory protein which plays a role in nuclear import of the pre-integration complex, transactivation of HIV LTR promoter, cell cycle arrest, and apoptosis during HIV infection.
Wagley, Madison C.
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