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Generating waxy rice starch with target type of amylopectin fine structure and gelatinization temperature by waxy gene editing

Carbohydrate Polymers, 2023
Waxy rice, which lacks amylose, is an important variant in rice, and its starches have been widely used. New waxy rice varieties generated via the CRISPR/Cas9 gene-editing system is described. Herein, four waxy rice starches with different physicochemical properties were successfully obtained by the CRISPR/Cas9 editing Waxy (Wx) gene.
Yuhao, Fu   +11 more
openaire   +2 more sources

Nucleotide polymorphisms in the waxy gene of NaN3-induced waxy rice mutants

Journal of Cereal Science, 2009
Abstract Spontaneous and induced waxy phenotype, associated with endosperm containing little or no amylose, has been recognized in rice ( Oryza sativa L.). Mutation of a dominant gene Wx into a recessive gene wx , which causes the inactivation or absence of granule bound starch synthase, is believed to be responsible for the change in endosperm ...
Toong Long Jeng   +4 more
openaire   +1 more source

Silencing of waxy genes in rice containing Wx transgenes

Molecular and General Genetics MGG, 1997
In order to study gene silencing in a monocot system we introduced a waxy (Wx) gene into rice. In the pollen grain of the transgenic wild-type plants, two types of Wx gene silencing were observed: Type I in which all the pollen grains showed the mutant (wx) phenotype, and Type II in which 50% of the pollen grains showed the wx phenotype. Analysis of Wx
K, Itoh, M, Nakajima, K, Shimamoto
openaire   +2 more sources

Molecular evidence for post-domestication selection in the Waxy gene of Chinese waxy maize

Molecular Breeding, 2008
Waxy maize was first reported in China in 1909 and is mainly used in food production in Asia. The evidence for strong domestication selection in the Waxy locus of rice and a selective sweep around its genomic region make us to wonder whether there has been similar selection in Waxy in glutinous maize. To address this issue, DNA sequences of Waxy, three
Longjiang Fan   +7 more
openaire   +1 more source

Isolation and characterization of the ryeWaxygene

Genome, 2009
Complete genomic and cDNA sequences of the Waxy gene encoding granule-bound starch synthase I (GBSSI) were isolated from the rye genome and characterized. The full-length rye Waxy genomic DNA and cDNA are 2767 bp and 1815 bp, respectively. The genomic sequence has 11 exons interrupted by 10 introns.
Jie, Xu   +5 more
openaire   +2 more sources

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