Results 1 to 10 of about 906,171 (200)
IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis [PDF]
Memorias do Instituto Oswaldo Cruz, 2010 The aim of this study was to evaluate the utility of western blot (WB) analysis as a diagnostic tool for congenital toxoplasmosis in 215 newborn infants.
Anderson S Machado +9 more
doaj +4 more sources
Autophagy Assays (LC3B immunofluorescence, LC3B western blot, acridine orange assay)
Bio-Protocol, 2013 Autophagy is a dynamic cellular event that is involved in the degradation of long lived proteins and organelles in cells. Biochemical methods such as western blot to measure autophagic proteins have been increasingly used in autophagy studies because it ...
Xin Zhang, Qingsong Liu
doaj +2 more sources
Imported intraocular gnathostomiasis with subretinal tracks confirmed by western blot assay. [PDF]
Korean J Parasitol, 2012 We report a case of intraocular gnathostomiasis diagnosed by western blot assay in a patient with subretinal tracks. A 15-year-old male patient complained of blurred vision in the right eye, lasting for 2 weeks. Eight months earlier, he had traveled to Vietnam for 1 week and ate raw wild boar meat and lobster. His best-corrected visual acuity was 20/20
Yang JH +5 more
europepmc +3 more sources
A Western Blot and Immunoprecipitation Assay to Verify Antibody Specificity [PDF]
BioTechniques, 2015 Life scientists are raising awareness of the problem of irreproducible data in basic research (1) (2). Part of the crisis of irreproducibility has been blamed on antibodies (3), and from this standpoint, an equal push to bring awareness to antibody ...
Sharon Bonnette
doaj +2 more sources
Comparisons of ELISA and Western blot assays for detection of autophagy flux [PDF]
Data in Brief, 2017 We analyzed autophagy/mitophagy flux in vitro (C2C12 myotubes) and in vivo (mouse skeletal muscle) following the treatments of autophagy inducers (starvation, rapamycin) and a mitophagy inducer (carbonyl cyanide m-chlorophenylhydrazone, CCCP) using two immunodetection methods, ELISA and Western blotting, and compared their working range, accuracy, and ...
Oh, Sung-hee +4 more
openaire +7 more sources
Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease [PDF]
Pathogens, 2021 Chagas disease is a debilitating and often fatal pathology resulting from infection by the protozoan parasite Trypanosoma cruzi. In its recommendations, the World Health Organization states that the diagnosis of T. cruzi infection is usually based on the
Jean-Yves Brossas +10 more
doaj +5 more sources
BPTF promotes tumor growth and predicts poor prognosis in lung adenocarcinomas. [PDF]
, 2015 BPTF, a subunit of NURF, is well known to be involved in the development of eukaryotic cell, but little is known about its roles in cancers, especially in non-small-cell lung cancer (NSCLC).
Dai, Meng +14 more
core +14 more sources
Kinase Activity-Tagged Western Blotting Assay [PDF]
BioTechniques, 2020 Determining cellular activities of protein kinases is a fundamental step for characterizing pathophysiological cell signaling pathways. Here, we optimized a nonradioactive method that detects protein kinases in tissues or cells after separation by SDS-PAGE and transfer onto polyvinylidene fluoride membranes.
Masumi Eto +3 more
openaire +3 more sources
The guideline for western blotting assay
Food Frontiers, 2022 AbstractWestern blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody reaction.
Fang Wang +5 more
openaire +2 more sources
Nuclear factor-kappa B inhibition can enhance apoptosis of differentiated thyroid cancer cells induced by 131I. [PDF]
PLoS ONE, 2012 OBJECTIVE: To evaluate changes of nuclear factor-kappa B (NF-κB) during radioiodine 131 ((131)I) therapy and whether NF-κB inhibition could enhance (131)I-induced apoptosis in differentiated thyroid cancer (DTC) cells in a synergistic manner.
Zhaowei Meng +5 more
doaj +1 more source