Aanalysis of Western blot band patterns in HIV-1 antibody positive cases
Pifu-xingbing zhenliaoxue zazhi, 2022 Objective To analyze the epidemiological characteristics and Western blot (WB) band patterns of HIV antibody in individuals with HIV-1 antibody positive in Jiangmen city, in order to provide scientific basis for the prevention and treatment of AIDS ...
Donghe ZHANG +3 more
doaj +1 more source
Kinase activity-tagged western blotting assay
BioTechniques, 2020 Determining cellular activities of protein kinases is a fundamental step for characterizing pathophysiological cell signaling pathways. Here, we optimized a nonradioactive method that detects protein kinases in tissues or cells after separation by SDS ...
Masumi Eto +3 more
doaj +1 more source
The myokine GDF-15 is a potential biomarker for myositis and associates with the protein aggregates of sporadic inclusion body myositis. [PDF]
, 2020 Background: The cytokine growth differentiation factor-15 (GDF-15) has been associated with inflammatory and mitochondrial disease, warranting exploration of its expression in myositis patients.
De Bleecker, Jan +2 more
core +2 more sources
The guideline for western blotting assay
Food Frontiers, 2022 Western blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody
Fang Wang +5 more
doaj +1 more source
Regulation of Expression of CXC Chemokines in Cultured Hepatocytes [PDF]
Iranian Journal of Public Health, 2005 Chemokines are low molecular weight proteins (8-17 kDa). They are classified in four distinct groups as CXC, CC, CX3C and C. Depending on the presence or absence of a motif called ELR (Arg-Leu-Glu) before the first cysteine residue in their structure ...
GhH Hassanshahiraviz +1 more
doaj +1 more source
Proliferating cell nuclear antigen in the cytoplasm interacts with components of glycolysis and cancer [PDF]
, 2010 Proliferating cell nuclear antigen (PCNA) is involved in a wide range of functions in the nucleus. However, a substantial amount of PCNA is also present in the cytoplasm, although their function is unknown.
Lee, Hoyun, Naryzhny, Stanislav N.
core +1 more source
Antibody Purification from Western Blotting
Bio-Protocol, 2012 This protocol describes a method of purifying antibodies from sera with denatured antigens immobilized on western blot membranes. Advantages include (1) fast and easy; (2) purification of antibody with antigen in denatured form allows high yield in case ...
Lin Fang
doaj +1 more source
AVNP2 protects against cognitive impairments induced by C6 glioma by suppressing tumour associated inflammation in rats [PDF]
, 2020 © 2020 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).Glioblastoma is a kind of malignant tumour and originates from the central nervous system.
Bankier +98 more
core +2 more sources
HIF-1α Contributes to Hypoxia-induced Invasion and Metastasis of Esophageal Carcinoma via Inhibiting E-cadherin and Promoting MMP-2 Expression [PDF]
, 2012 Hypoxia-inducible factor-1α (HIF-1α) has been found to enhance tumor invasion and metastasis, but no study has reported its action in esophageal carcinoma.
Cheng, Yun-Jie +7 more
core +1 more source
Microfluidic Western blotting [PDF]
Proceedings of the National Academy of Sciences, 2012 Rapid, quantitative Western blotting is a long-sought bioanalytical goal in the life sciences. To this end, we describe a Western blotting assay conducted in a single glass microchannel under purely electronic control. The μWestern blot is comprised of multiple steps: sample enrichment, protein sizing, protein immobilization (blotting), and in situ ...
Amy E. Herr, Alex J. Hughes
openaire +3 more sources