Results 61 to 70 of about 2,778,272 (379)

Selective targeting of activating and inhibitory Smads by distinct WWP2 ubiquitin ligase isoforms differentially modulates TGFβ signalling and EMT [PDF]

, 2011
Ubiquitin-dependent mechanisms have emerged as essential regulatory elements controlling cellular levels of Smads and TGFß-dependent biological outputs such as epithelial–mesenchymal transition (EMT). In this study, we identify a HECT E3 ubiquitin ligase
A Chantry, A Hata, CH Heldin, CS Lutz, D Javelaud, F Bernassola, F Murray-Zmijewski, FJ McDonald, G Pirozzi, H Li, H Xu, H Zhu, HM Xu, J Tazi, J Xu, J Zavadil, L Levy, M Flasza, NJ Foot, NS Raikwar, P Kavsak, P Lönn, PJ Lu, S Bonni, S Danckwardt, S Hong, S M Soond, S Wicks, S Wiesner, S Zhang, T Atsumi, T Mund, TW Nilsen, U Valcourt, V Ellenrieder, W Li   +35 more
core   +1 more source

Western blot

, 2022
western blotting analysis of CD63,CD81,TSG101,calnexin,CD56,perforin and ...
openaire   +1 more source

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting.

Journal of Visualized Experiments, 2019
Western blotting is a technique that is commonly used to detect and quantify protein expression. Over the years, this technique has led to many advances in both basic and clinical research.
Yu-Ting Huang, Dinja van der Hoorn, Leire M Ledahawsky, Anna A L Motyl, Crispin Y Jordan, T. Gillingwater, E. Groen   +6 more
semanticscholar   +1 more source

Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing [PDF]

, 2015
The activity of typical 2-cys peroxiredoxin (Prxs) can be regulated by hyperoxidation with a consequent loss of redox activity. Here we developed a simple assay to monitor the level of hyperoxidation of different typical 2-cys prxs simultaneously.
Bulleid, Neil J., Cao, Zhenbo
core   +1 more source

Interaction vesicles as emerging mediators of host‐pathogen molecular crosstalk and their implications for infection dynamics

FEBS Letters, EarlyView.
Interaction extracellular vesicles (iEVs) are hybrid vesicles formed through host‐pathogen communication. They facilitate immune evasion, transfer pathogens' molecules, increase host cell uptake, and enhance virulence. This Perspective article illustrates the multifunctional roles of iEVs and highlights their emerging relevance in infection dynamics ...
Bruna Sabatke, Izadora Volpato Rossi, Marcel I. Ramirez   +2 more
wiley   +1 more source

Western Blot diagnostic yield for simultaneous antibody detection in patients with human cysticercosis, hydatidosis, and human fascioliasis

Revista Peruana de Medicina Experimental y Salud Pública, 2016
Objectives. To determine the diagnostic yield using western blotting to simultaneously detect antibodies in patients with human cysticercosis, hydatidosis, and human fascioliasis. Materials and methods.
Kelly Davelois, Hermes Escalante, César Jara   +2 more
doaj   +1 more source

Development and evaluation of an indirect ELISA for detection of Teladorsagia circumcincta infection in sheep

BMC Veterinary Research, 2021
Background The gastrointestinal helminth, Teladorsagia circumcincta, is one of the major health risks and production-limiting diseases in small ruminant populations, particularly in temperate regions.
Jalal Aliabadi, Ehsan Rakhshandehroo, Azadeh Yektaseresht   +2 more
doaj   +1 more source

Optimization of Chemoenzymatic Mass Tagging by Strain-Promoted Cycloaddition (SPAAC) for the Determination of O-GlcNAc Stoichiometry by Western Blotting.

Biochemistry, 2018
The dynamic modification of intracellular proteins by O-linked β -N-acetylglucosamine (O-GlcNAcylation) plays critical roles in many cellular processes.
Narek Darabedian, John W. Thompson, Kelly N. Chuh, L. Hsieh‐Wilson, Matthew R. Pratt   +4 more
semanticscholar   +1 more source

SUMO chain-induced dimerization activates RNF4 [PDF]

, 2014
Dimeric RING E3 ligases interact with protein substrates and conformationally restrain the ubiquitin-E2-conjugating enzyme thioester complex such that it is primed for catalysis.
Alejandro Rojas-Fernandez, Anna Plechanovová, Brzovic, Buchwald, Budhidarmo, Calderon-Villalobos, de Bie, Deshaies, Desterro, Desterro, Dou, Dou, Dueber, Ellis Jaffray, Feltham, Galanty, Geiss-Friedlander, Geoffroy, Geoffroy, Goddard, Goddard, Golebiowski, Guzzo, Haas, Hattersley, Hay, Hecker, Hickey, Johnson, Kang, Kravtsova-Ivantsiv, Lallemand-Breitenbach, Liew, Linke, Mace, Martin, Melchior, Michael H. Tatham, Mu, Mukhopadhyay, Mukhopadhyay, Neil Hattersley, Perry, Plechanovová, Plechanovová, Pruneda, Rodriguez, Ronald T. Hay, Saitoh, Sampson, Scheffner, Schermelleh, Shen, Song, Spencer, Sun, Tatham, Tatham, Tatham, Tatham, Vyas, Wenzel, Yin, Yin, Zhang   +64 more
core   +2 more sources

Protonophore activity of short‐chain fatty acids induces their intracellular accumulation and acidification

FEBS Letters, EarlyView.
The protonated form of butyrate, as well as other short‐chain fatty acids (SCFAs), is membrane permeable. In acidic extracellular environments, this can lead to intracellular accumulation of SCFAs and cytosolic acidification. This phenomenon will be particularly relevant in acidic environments such as the large intestine or tumor microenvironments ...
Muwei Jiang, Frans Bianchi, Geert van den Bogaart   +2 more
wiley   +1 more source