Results 61 to 70 of about 667,007 (283)

Western blotting [PDF]

open access: yes, 2009
タンパク質を特異的に検出するウェスタン分析は,化学発光を用いる検出法により微量のタンパク質でも強いシグナルが得られるため,強力な実験手法である.本章では,ウェスタン分析の実験手法と抗体の作製法について紹介する.Western blotting is a powerful tool for detecting with high sensitivity a specific protein resolved by SDS-polyacrylamide gel electrophoresis.
高橋, 裕一郎, 大西, 岳人
core  

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

open access: yesMolecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat   +8 more
wiley   +1 more source

Evidence for the existence of Dm0-like Lamin in Sf9 cells

open access: yes浙江大学学报. 农业与生命科学版, 2015
The nuclear membrane of mammalian cells was composed of inner nuclear membrane, outer nuclear membrane and perinuclear space. The lamina was localized under the nucleoplasm face of inner nuclear membrane. It has been known that the lamina was distributed
Wei Wenqiang, Ji Shaoping, Zhang Yinyan
doaj   +1 more source

Caracterización del patrón proteico y perfil antigénico de larvas L3 de anisákidos de importancia médica aisladas de peces comerciales de la costa peruana

open access: yesAnales de la Facultad de Medicina, 2020
Introducción. La anisakidosis es una zoonosis causada por la ingestión accidental de larvas (L3) de anisákidos. Objetivo. Caracterizar el patrón proteico y perfil antigénico de la L3 de Anisakis simplex s.l. (tipo I), A. physeteris s.l.
Rosa Martínez-Rojas   +3 more
doaj   +1 more source

ELISA and western blotting data

open access: yes, 2019
Data of in vitro and in vivo experiments from ELISA, western blotting and immunostaining ...
Plinio Casarotto (3992108)
core   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

open access: yesMolecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano   +6 more
wiley   +1 more source

Evaluation of the Western blotting method for the diagnosis of congenital toxoplasmosis

open access: yesJornal de Pediatria (Versão em Português), 2016
Objective: To evaluate the Western blotting method for the detection of IgG anti‐Toxoplasma gondii (T. gondii) (IgG‐WB) in the serum of children with suspected congenital toxoplasmosis.
Jaqueline Dario Capobiango   +6 more
doaj   +1 more source

COMP–PMEPA1 axis promotes epithelial‐to‐mesenchymal transition in breast cancer cells

open access: yesMolecular Oncology, EarlyView.
This study reveals that cartilage oligomeric matrix protein (COMP) promotes epithelial‐to‐mesenchymal transition (EMT) in breast cancer. We identify PMEPA1 (protein TMEPAI) as a novel COMP‐binding partner that mediates EMT via binding to the TSP domains of COMP, establishing the COMP–PMEPA1 axis as a key EMT driver in breast cancer.
Konstantinos S. Papadakos   +6 more
wiley   +1 more source

DEHYDRIN PROFILES OF SOME IRANIAN MELON VARIETIES (Cucumis melo L. Merr) UNDER DROUGHT STRE SS CONDITIONS

open access: yesActa Scientiarum Polonorum: Hortorum Cultus, 2019
Dehydrins are proteins that play a role in the mechanism of drought tolerance. This study aimed at establishing dehydrin profile and accumulation in four local melon varieties of Iran: Mino, Dargazi, Saveii, and Semsori, as well as in a commercial ...
Alireza Motallebi-Azar   +2 more
doaj   +1 more source

Original Western blotting analysis

open access: yes, 2019
Western blotting analysis confirms the presence of the enzyme TlicBgluc1 in colony expression supernatant 3 to 5 with a single band immuno-labeled with the corresponding approximate mW.
Ana Elizabeth Araújo (7860695)
core   +1 more source

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