Results 81 to 90 of about 3,779,882 (303)

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source

Whole genome sequencing in Mycobacterium tuberculosis

Annals of Translational Medicine, 2019
Fighting the emergence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis (TB) requires a proper and personalized use of new antitubercular drugs. To achieve this goal, the sensitivity profile to antitubercular drug should be available in a short-time and results should be highly reliable be used for patient-care.
Andrea, Spitaleri, Arash, Ghodousi, Paolo, Miotto, Daniela Maria, Cirillo   +3 more
openaire   +2 more sources

Whole-Genome Sequencing in Healthy People [PDF]

Mayo Clinic Proceedings, 2017
Recent technological advances have radically changed genetic testing from an expensive and burdensome undertaking to a rapid and less costly option for many purposes. The utility of "next-generation" sequencing has been found to establish the diagnosis for hundreds of genetic disorders, to assess pharmacogenomic variants, and to identify treatable ...
Lindor, Noralane M., Thibodeau, Stephen N., Burke, Wylie   +2 more
openaire   +2 more sources

Approaches to informed consent for hypothesis-testing and hypothesis-generating clinical genomics research

BMC Medical Genomics, 2012
Background Massively-parallel sequencing (MPS) technologies create challenges for informed consent of research participants given the enormous scale of the data and the wide range of potential results.
Facio Flavia M, Sapp Julie C, Linn Amy, Biesecker Leslie G   +3 more
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Structural variation in the chicken genome identified by paired-end next-generation DNA sequencing of reduced representation libraries [PDF]

, 2011
Background Variation within individual genomes ranges from single nucleotide polymorphisms (SNPs) to kilobase, and even megabase, sized structural variants (SVs), such as deletions, insertions, inversions, and more complex rearrangements.
Kerstens Hindrik HD, Vereijken, A., Martien AM Groenen, Bert W Dibbits, Groenen, M.A.M., Dibbits, B.W., Kerstens, H.H.D., Dibbits Bert W, Okimoto Ron, Hindrik HD Kerstens, Richard PMA Crooijmans, Addie Vereijken, Vereijken Addie, Crooijmans, R.P.M.A., Okimoto, R., Groenen Martien AM, Crooijmans Richard PMA, Ron Okimoto   +17 more
core   +2 more sources

Whole‐Genome Sequencing of Yeast Cells

Current Protocols in Molecular Biology, 2019
AbstractThe budding yeast, Saccharomyces cerevisiae, has been widely used for genetic studies of fundamental cellular functions. The isolation and analysis of yeast mutants is a commonly used and powerful technique to identify the genes that are involved in a process of interest.
Rajaraman, Gopalakrishnan, Fred, Winston
openaire   +3 more sources

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

The development and characterization of a 60K SNP chip for chicken [PDF]

, 2011
Background In livestock species like the chicken, high throughput single nucleotide polymorphism (SNP) genotyping assays are increasingly being used for whole genome association studies and as a tool in breeding (referred to as genomic selection).
Vereijken, Addie, Wesley C Warren, William M Muir, Hillier LaDeana W, LaDeana W Hillier, Zare Yalda, Groenen, Martien AM, Hillier, LaDeana W, Hans H Cheng, Groenen, Martin AM, Vereijken Addie, Muir William M, Zare, Yalda, Cheng, Hans H, Groenen Martien AM, Megens, H.J.W.C., Cheng Hans H, Megens Hendrik-Jan, Ron Okimoto, Vereijken, A., Muir, W.M., Martien AM Groenen, Groenen, M.A.M., Hendrik-Jan Megens, Warren Wesley C, Crooijmans, Richard PMA, Yalda Zare, Warren, W.C., Warren, Wesley C, Zare, Y., Okimoto Ron, Cheng, H.H., Richard PMA Crooijmans, Addie Vereijken, Hillier, L.W., Okimoto, Ron, Muir, Williams M, Crooijmans, R.P.M.A., Okimoto, R., Megens, Hendrik-Jan, Muir, William M, Crooijmans Richard PMA   +41 more
core   +2 more sources

Whole-Genome Sequencing in Outbreak Analysis [PDF]

Clinical Microbiology Reviews, 2015
SUMMARYIn addition to the ever-present concern of medical professionals about epidemics of infectious diseases, the relative ease of access and low cost of obtaining, producing, and disseminating pathogenic organisms or biological toxins mean that bioterrorism activity should also be considered when facing a disease outbreak.
Carol A, Gilchrist, Stephen D, Turner, Margaret F, Riley, William A, Petri, Erik L, Hewlett   +4 more
openaire   +2 more sources