Results 81 to 90 of about 86,476 (288)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Phylogeny and Multiple Independent Whole-Genome Duplication Events in the Brassicales [PDF]

American Journal of Botany, 2019
ABSTRACT Whole-genome duplications (WGDs) are prevalent throughout the evolutionary history of plants. For example, dozens of WGDs have been phylogenetically localized across the order Brassicales, specifically, within the family Brassicaceae.
Mabry, Makenzie E., Brose, Julia M., Blischak, Paul D., Sutherland, Brittany, Dismukes, Wade T., Bottoms, Christopher A., Edger, Patrick P., Washburn, Jacob D., An, Hong, Hall, Jocelyn C., McKain, Michael R., Al-Shehbaz, Ihsan, Barker, Michael S., Schranz, M. Eric, Conant, Gavin C., Pires, J. Chris   +15 more
openaire   +2 more sources

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Spontaneous whole-genome duplication restores fertility in interspecific hybrids

Nature Communications, 2019
Hybridization across species can lead to offspring with reduced fertility. Here, the authors experimentally evolve yeast and show that whole-genome duplication during asexual reproduction can restore fertility in hybrids over a relatively short ...
Guillaume Charron, Souhir Marsit, Mathieu Hénault, Hélène Martin, Christian R. Landry   +4 more
doaj   +1 more source

The Phenotype Paradox: Lessons From Natural Transcriptome Evolution on How to Engineer Plants

Frontiers in Plant Science, 2020
Plants have evolved genome complexity through iterative rounds of single gene and whole genome duplication. This has led to substantial expansion in transcription factor numbers following preferential retention and subsequent functional divergence of ...
Justin Law, Kangbo Ng, Kangbo Ng, Oliver P. F. Windram   +3 more
doaj   +1 more source

A European Whitefish Linkage Map and Its Implications for Understanding Genome-Wide Synteny Between Salmonids Following Whole Genome Duplication [PDF]

, 2018
Genomic datasets continue to increase in number due to the ease of production for a wider selection of species including non-model organisms. For many of these species, especially those with large or polyploid genomes, highly contiguous and well ...
De-Kayne, Rishi, Feulner, Philine, Philine G. D. Feulner, Rishi De-Kayne   +3 more
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Gene-interleaving patterns of synteny in the Saccharomyces cerevisiae genome: are they proof of an ancient genome duplication event?

Biology Direct, 2007
Background Recent comparative genomic studies claim local syntenic gene-interleaving relationships in Ashbya gossypii and Kluyveromyces waltii are compelling evidence for an ancient whole-genome duplication event in Saccharomyces cerevisiae. We here test,
Sun Feng-Jie, LeDuc Richard, Ruedi Elizabeth A, Martin Nicolas, Caetano-Anollés Gustavo   +4 more
doaj   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

Whole-genome duplication events of four palms.

, 2015
(A) The distribution of synonymous substitution rate (Ks) of the paralogs is displayed in red and blue for N. fruticans and P. dactylifera, respectively. Peaks I and II indicated two whole-genome duplication events in both species. The yellow bars showed
Zhang Zhang (14172), Wuxia Guo (838796), Renchao Zhou (82360), Ziwen He (217976), Suhua Shi (66594), Ying Zhang (40767)   +5 more
core   +1 more source