Results 61 to 70 of about 510,094 (313)

Circulating tumor cell viability during and after radiotherapy mirrors treatment response in cancer patients

Molecular Oncology, EarlyView.
Radiotherapy (RT) response depends on the DNA repair capacity of tumor and host cells. We show that circulating tumor cell (CTC) counts and apoptosis rates before and after RT predict treatment response and outcome, which can be accessed via easily accessible liquid biopsy approaches. Created in BioRender. Wikman, H.
Yvonne Goy, Jana Löptien, Cornelia Coith, Afroditi Nanou, Katharina Hintelmann, Pinnschmidt Hans, Cordula Petersen, Klaus Pantel, Sabine Riethdorf, Kerstin Borgmann, Harriet Wikman   +10 more
wiley   +1 more source

Accelerated X-ray Structure Elucidation of a 36 kDa Muramidase/Transglycosylase Using wARP [PDF]

, 1998
The X-ray structure of the 36kDa soluble lytic transglycosylase from Escherichia coli has been determined starting with the multiple isomorphous replacement method with inclusion of anomalous scattering at 2.7 Å resolution. Subsequently, before any model
Perrakis, A., Dijkstra, Bauke W., Perrakis, Anastassis, van Asselt, E. J., Kalk, K. H., Lamzin, V. S., Kalk, Kor H., Lamzin, Victor S.,, van Asselt, Erik J., Dijkstra, B. W., Asselt, Erik J. van,, Kalk, Kor H.,, Perrakis, Anastassis,, Dijkstra, Bauke W.,, Lamzin, Victor S.   +14 more
core   +1 more source

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Single crystal x-ray diffraction studies on small, medium and large molecules [PDF]

, 1996
Chapter 1. Production of crystals for diffraction analysis would be assisted by the devising of a set of rules which, given molecular formula, could predict crystal formation conditions.
Butterworth, Susanna
core  

EFEITO DO DIOXIDO DE CARBONO SOBRE A ESTABILIDADE DA FASE Ca2Fe2O5 APLICADA NO PROCESSO DE DEGRADAÇÃO FOTOCATALITICO DO AZUL DE METILENO

Química Nova, 2015
Solid samples containing a Ca2Fe2O5 phase were synthesized using the Pechini method. The samples were characterized using X-ray diffraction, thermogravimetric analysis, differential thermal analysis, X-ray fluorescence, nitrogen adsorption/desorption ...
Erandir Brasil da Silva, Antoninho Valentini   +1 more
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

A Phase-resolved View of Millihertz Quasiperiodic Oscillations in the Ultraluminous X-Ray Source M51 ULX-7: Evidence for a Magnetically Truncated Disk and Geometrical Beaming

The Astrophysical Journal
X-ray quasiperiodic oscillations (QPOs) are commonly observed in Galactic X-ray binaries and extragalactic ultraluminous X-ray sources (ULXs). In this study, we perform a phase-resolved analysis of recently discovered X-ray mHz QPOs in M51 ULX-7.
Qing-Cang Shui, Shu Zhang, Hua Feng, Yu-Peng Chen, Shuang-Nan Zhang, Jing-Qiang Peng   +5 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source