Modelling human genetic disorders in Xenopus tropicalis
Disease Models & MechanismsABSTRACT Recent progress in human disease genetics is leading to rapid advances in understanding pathobiological mechanisms. However, the sheer number of risk-conveying genetic variants being identified demands in vivo model systems that are amenable to functional analyses at scale. Here we provide a practical guide for using the diploid
Helen Rankin Willsey +5 more
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Evolutionary divergence in tail regeneration between Xenopus laevis and Xenopus tropicalis [PDF]
Cell & Bioscience, 2021 Tissue regeneration is of fast growing importance in the development of biomedicine, particularly organ replacement therapies. Unfortunately, many human organs cannot regenerate.
Shouhong Wang, Yun-Bo Shi
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Melanin deposition and key molecular features in Xenopus tropicalis oocytes [PDF]
BMC BiologyBackground Melanin pigmentation in oocytes is a critical feature for both the esthetic and developmental aspects of oocytes, influencing their polarity and overall development.
Hongyang Yi +11 more
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Revealing mitf functions and visualizing allografted tumor metastasis in colorless and immunodeficient Xenopus tropicalis [PDF]
Communications BiologyTransparent immunodeficient animal models not only enhance in vivo imaging investigations of visceral organ development but also facilitate in vivo tracking of transplanted tumor cells.
Rensen Ran +5 more
doaj +3 more sources
CRISPR/Cas9-Mediated Models of Retinitis Pigmentosa Reveal Differential Proliferative Response of Müller Cells between Xenopus laevis and Xenopus tropicalis [PDF]
Cells, 2022 Retinitis pigmentosa is an inherited retinal dystrophy that ultimately leads to blindness due to the progressive degeneration of rod photoreceptors and the subsequent non-cell autonomous death of cones.
Karine Parain +7 more
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Metamorphic gene regulation programs in Xenopus tropicalis tadpole brain [PDF]
PLoS ONE, 2023 Amphibian metamorphosis is controlled by thyroid hormone (TH), which binds TH receptors (TRs) to regulate gene expression programs that underlie morphogenesis.
Samhitha Raj +3 more
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Echocardiographic assessment of Xenopus tropicalis heart regeneration [PDF]
Cell & Bioscience, 2023 Background Recently, it was reported that the adult X. tropicalis heart can regenerate in a nearly scar-free manner after injury via apical resection. Thus, a cardiac regeneration model in adult X. tropicalis provides a powerful tool for recapitulating a
Luocheng Lv +13 more
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Developing immortal cell lines from Xenopus embryos, four novel cell lines derived from Xenopus tropicalis [PDF]
Open Biology, 2022 The diploid anuran Xenopus tropicalis has emerged as a key research model in cell and developmental biology. To enhance the usefulness of this species, we developed methods for generating immortal cell lines from Nigerian strain (NXR_1018, RRID ...
Gary J. Gorbsky +8 more
doaj +3 more sources
Expanding the CRISPR/Cas genome-editing scope in Xenopus tropicalis [PDF]
Cell & Bioscience, 2022 Background The true diploid frog, Xenopus tropicalis (X. tropicalis) is an excellent genetic model organism. To date, the CRISPR/Cas-mediated genome editing methods established in this species are mostly based on SpCas9 that requires the stringent NGG ...
Zhaoying Shi +5 more
doaj +3 more sources
Isolation and evaluation of erythroid progenitors in the livers of larval, froglet, and adult Xenopus tropicalis. [PDF]
Biol Open, 2023 Xenopus liver maintains erythropoietic activity from the larval to the adult stage. During metamorphosis, thyroid hormone mediates apoptosis of larval-type erythroid progenitors and proliferation of adult-type erythroid progenitors, and a globin switch ...
Omata K +8 more
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