The Tmem16a chloride channel is required for mucin maturation after secretion from goblet-like cells in the Xenopus tropicalis tadpole skin [PDF]
Scientific ReportsThe TMEM16A chloride channel is proposed as a therapeutic target in cystic fibrosis, where activation of this ion channel might restore airway surface hydration and mitigate respiratory symptoms.
Eamon Dubaissi +10 more
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Animal Maintenance Systems: Xenopus tropicalis. [PDF]
Cold Spring Harb Protoc, 2020 Modular recirculating animal aquaculture systems incorporate UV sterilization and biological, mechanical, and activated carbon filtration, creating a nearly self-contained stable housing environment for Xenopus tropicalis. Nonetheless, minimal water exchange is necessary to mitigate accumulation of metabolic waste, and regular weekly, monthly, and ...
Shaidani NI +3 more
europepmc +5 more sources
Revealing mitf functions and visualizing allografted tumor metastasis in colorless and immunodeficient Xenopus tropicalis [PDF]
Communications BiologyTransparent immunodeficient animal models not only enhance in vivo imaging investigations of visceral organ development but also facilitate in vivo tracking of transplanted tumor cells.
Rensen Ran +5 more
doaj +4 more sources
Protocol for tail vein injection in Xenopus tropicalis tadpoles [PDF]
STAR ProtocolsSummary: Functional studies in post-embryonic Xenopus tadpoles are challenging because embryonic perturbations often lead to developmental consequences, such as lethality.
Jeet H. Patel +4 more
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, 2020 Published as part of Ayoro, Halamoussa Joëlle, Segniagbeto, Gabriel Hoinsoudé, Hema, Emmanuel Midibahaye, Penner, Johannes, Oueda, Adama, Dubois, Alain, Rödel, Mark-Oliver, Kabré, Gustave Boureima & Ohler, Annemarie, 2020, List of amphibian species (Vertebrata, Tetrapoda) of Burkina Faso, pp.
Ayoro, Halamoussa Joëlle +8 more
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Metamorphic gene regulation programs in Xenopus tropicalis tadpole brain [PDF]
PLoS ONE, 2023 Amphibian metamorphosis is controlled by thyroid hormone (TH), which binds TH receptors (TRs) to regulate gene expression programs that underlie morphogenesis.
Samhitha Raj +3 more
doaj +4 more sources
Expanding the CRISPR/Cas genome-editing scope in Xenopus tropicalis [PDF]
Cell & Bioscience, 2022 Background The true diploid frog, Xenopus tropicalis (X. tropicalis) is an excellent genetic model organism. To date, the CRISPR/Cas-mediated genome editing methods established in this species are mostly based on SpCas9 that requires the stringent NGG ...
Zhaoying Shi +5 more
doaj +2 more sources
Whole-genome sequencing identifies I-SceI-mediated transgene integration sites in Xenopus tropicalis snai2:eGFP line [PDF]
G3: Genes, Genomes, Genetics, 2022 Transgenesis with the meganuclease I-SceI is a safe and efficient method, but the underlying mechanisms remain unclear due to the lack of information on transgene localization.
Jian Wang, Congyu Lu, Shuo Wei
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Cardiac regeneration in Xenopus tropicalis and Xenopus laevis: discrepancies and problems [PDF]
Cell & Bioscience, 2018 Two studies have recently focused on adult heart regeneration in Xenopus. While we reported on cardiac myogenic regeneration in Xenopus tropicalis after injury, Marshall and colleagues found no regeneration in an injured heart in Xenopus laevis. Here, we
Souqi Liao +5 more
doaj +3 more sources
Comparison of TALEN scaffolds in Xenopus tropicalis [PDF]
Biology Open, 2013 Summary Transcription activator-like effector nucleases (TALENs) are facile and potent tools used to modify a gene of interest for targeted gene knockout.
Keisuke Nakajima, Yoshio Yaoita
doaj +3 more sources