Structure and function of the lanthanide-dependent methanol dehydrogenase XoxF from the methanotroph Methylomicrobium buryatense 5GB1C [PDF]
In methylotrophic bacteria, which use one-carbon (C1) compounds as a carbon source, methanol is oxidized by pyrroloquinoline quinone (PQQ)-dependent methanol dehydrogenase (MDH) enzymes. Methylotrophic genomes generally encode two distinct MDHs, MxaF and XoxF.
Y. Deng, S. Ro, A. Rosenzweig
semanticscholar +3 more sources
XoxF-Type Methanol Dehydrogenase from the Anaerobic Methanotroph “Candidatus Methylomirabilis oxyfera” [PDF]
ABSTRACT “ Candidatus Methylomirabilis oxyfera” is a newly discovered anaerobic methanotroph that, surprisingly, oxidizes methane through an aerobic methane oxidation pathway. The second step in this aerobic pathway is the oxidation of methanol.
Ming L. Wu +6 more
semanticscholar +5 more sources
XoxF Is Required for Expression of Methanol Dehydrogenase in Methylobacterium extorquens AM1 [PDF]
ABSTRACT In Gram-negative methylotrophic bacteria, the first step in methylotrophic growth is the oxidation of methanol to formaldehyde in the periplasm by methanol dehydrogenase. In most organisms studied to date, this enzyme consists of the MxaF and MxaI proteins, which make up the large and small subunits of this ...
E. Skovran +4 more
semanticscholar +3 more sources
Characterization of a novel cytochrome cGJ as the electron acceptor of XoxF-MDH in the thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV. [PDF]
Methanotrophs play a prominent role in the global carbon cycle, by oxidizing the potent greenhouse gas methane to CO2. Methane is first converted into methanol by methane monooxygenase. This methanol is subsequently oxidized by either a calcium-dependent MxaF-type or a lanthanide-dependent XoxF-type methanol dehydrogenase (MDH). Electrons from methanol
W. Versantvoort +8 more
semanticscholar +3 more sources
Investigation of XoxF methanol dehydrogenases reveals new methylotrophic bacteria in pelagic marine and freshwater ecosystems. [PDF]
The diversity and distribution of methylotrophic bacteria have been investigated in the oceans and lakes using the methanol dehydrogenase mxaF gene as a functional marker. However, pelagic marine (OM43) and freshwater (LD28 and PRD01a001B) methylotrophs within the Betaproteobacteria lack mxaF, instead possessing a related xoxF4-encoded methanol ...
A. Ramachandran, D. Walsh
semanticscholar +3 more sources
Characterisation of novel methylotrophs and the role of xoxF in coastal marine environments [PDF]
Methanol is one of the most abundant volatile organic gases in the atmosphere, and whilst much is known about the sources of methanol, much less is known about the sinks. Methylotrophs are able to use one carbon compounds, such as methanol, as their sole
Howat, Alexandra M.
core +2 more sources
XoxF encoding an alternative methanol dehydrogenase is widespread in coastal marine environments. [PDF]
Summary The xox F gene, encoding a pyrroloquinoline quinone‐dependent methanol dehydrogenase, is found in all known proteobacterial methylotrophs.
Martin Taubert +7 more
semanticscholar +4 more sources
Impact of Copper on the Physiology and Transcriptome of Methylosinus Trichosporium OB3b Grown on Either Methane or Methanol. [PDF]
Methylosinus trichosporium OB3b can grow on methanol in the absence but not presence of copper due: (1) overgeneration of reducing power from methanol oxidation; (2) formate accumulation leading to acidification of the growth medium and inactivation of formate dehydrogenase; and (3) lack of expression of potential oxidative stress defence genes ...
Peng P, Semrau JD.
europepmc +2 more sources
Influence of Rare Earth Elements on Prebiotic Reaction Networks Resembling the Biologically Relevant Krebs Cycle. [PDF]
Rare earth elements can act as mediators in a prebiotic reaction network, producing various intermediates of the citric acid cycle starting from glyoxylate and pyruvate. The ionic radius and the resulting variation in Lewis acidity has a significant influence on the observed product scope.
Gutenthaler-Tietze J +2 more
europepmc +2 more sources
C3 Compound Metabolism in the Thermoacidophilic Methanotroph Methylacidiphilum fumariolicum SolV. [PDF]
Terrestrial and oceanic geothermal areas emit substantial amounts of small hydrocarbons. The thermoacidophilic verrucomicrobial methanotroph Methylacidiphilum fumariolicum SolV, isolated from the Solfatara volcano, was shown to grow on 2‐propanol and acetone using steady‐state continuous cultures.
Liu C +7 more
europepmc +2 more sources

