Neodymium as Metal Cofactor for Biological Methanol Oxidation: Structure and Kinetics of an XoxF1-Type Methanol Dehydrogenase [PDF]
mBio, 2021 Lanthanides comprise a group of 15 elements with atomic numbers 57 to 71 that are essential in a variety of high-tech devices, such as mobile phones, but were considered biologically inert for a long time.
Rob A. Schmitz +9 more
doaj +7 more sources
NAD-dependent, PQQ-containing methanol dehydrogenase: a bacterial dehydrogenase in a multienzyme complex [PDF]
FEBS Letters, 1984 Cell‐free extracts of methanol‐grown Nocardia sp. 239 only show significant dye‐linked methanol‐oxidizing activity when NAD+ is added to the assay mixture. This activity resides in a multienzyme complex which could be resolved into 3 components, namely the methanol dehydrogenase, NAD‐dependent aldehyde dehydrogenase and NADH dehydrogenase.
J A Duine
exaly +4 more sources
Discovery and Biochemical Characterization of a Methanol Dehydrogenase From Lysinibacillus xylanilyticus [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 Bioconversion of C1 chemicals such as methane and methanol into higher carbon-chain chemicals has been widely studied. Methanol oxidation catalyzed by methanol dehydrogenase (Mdh) is one of the key steps in methanol utilization in bacterial methylotrophy.
Jin-Young Lee +19 more
doaj +3 more sources
Deciphering the assembly process of PQQ dependent methanol dehydrogenase [PDF]
Nature CommunicationsPyrroloquinoline quinone (PQQ)-dependent methanol dehydrogenases (MDHs), the periplasmic metalloenzymes in Gram-negative methylotrophic bacteria, play a pivotal role in methane and methanol bio-utilization.
Haichuan Zhou +16 more
doaj +4 more sources
Switching Between Methanol Accumulation and Cell Growth by Expression Control of Methanol Dehydrogenase in Methylosinus trichosporium OB3b Mutant [PDF]
Frontiers in Microbiology, 2021 Methanotrophs have been used to convert methane to methanol at ambient temperature and pressure. In order to accumulate methanol using methanotrophs, methanol dehydrogenase (MDH) must be downregulated as it consumes methanol. Here, we describe a methanol
Hidehiro Ito +5 more
doaj +2 more sources
Autotrophic methylotrophy with no methanol dehydrogenase (MDH) in a strain of fluorescent Pseudomonas [PDF]
PeerJBackground Very few true Pseudomonas methylotrophic strains have been described, and in none of them have the pathways for one-carbon (C1) substrate metabolism been elucidated.
Paolo De Marco
doaj +3 more sources
Directed evolution of a neutrophilic and mesophilic methanol dehydrogenase based on high-throughput and accurate measurement of formaldehyde [PDF]
Synthetic and Systems Biotechnology, 2023 Methanol is a promising one-carbon feedstock for biomanufacturing, which can be sustainably produced from carbon dioxide and natural gas. However, the efficiency of methanol bioconversion is limited by the poor catalytic properties of nicotinamide ...
Jin Qian +13 more
doaj +2 more sources
Methanol Dehydrogenases as a Key Biocatalysts for Synthetic Methylotrophy [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 One-carbon (C1) chemicals are potential building blocks for cheap and sustainable re-sources such as methane, methanol, formaldehyde, formate, carbon monoxide, and more.
Thien-Kim Le +5 more
doaj +3 more sources
MxaY regulates the lanthanide-mediated methanol dehydrogenase switch in Methylomicrobium buryatense [PDF]
PeerJ, 2016 Many methylotrophs, microorganisms that consume carbon compounds lacking carbon–carbon bonds, use two different systems to oxidize methanol for energy production and biomass accumulation.
Frances Chu +2 more
doaj +3 more sources
Advances in synthetic biology for engineering methylotrophic microbial cell factories [PDF]
Journal of BacteriologyMethanol, a renewable non-food C1 substrate, holds great promise as a feedstock for sustainable biomanufacturing and carbon neutral production. However, its industrial application is hindered by low methanol assimilation efficiency in most microbes ...
Liang Guo +4 more
doaj +2 more sources