Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2 [PDF]
ACS Synthetic Biology, 2019 Synthetic methylotrophy, the modification of organisms such as E. coli to grow on methanol, is a longstanding goal of metabolic engineering and synthetic biology.
Benjamin M Woolston +2 more
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Role of Two Methanol Dehydrogenases in Methanol Metabolism of Methylobacterium
Shipin gongye ke-jiIn this study, the pyrroloquinoline quinone (PQQ) high-yield mutant J1-1 of Methylobacterium glucophilum MP688 was used to do bioinformatics analysis to extract the two methanol dehydrogenase (MDH) genes from the genome, mpq0771 and mpq2496.
Yaqing GUO +5 more
doaj +2 more sources
A catalytic role of XoxF1 as La3+-dependent methanol dehydrogenase in Methylobacterium extorquens strain AM1. [PDF]
PLoS ONE, 2012 In the methylotrophic bacterium Methylobacterium extorquens strain AM1, MxaF, a Ca(2+)-dependent methanol dehydrogenase (MDH), is the main enzyme catalyzing methanol oxidation during growth on methanol.
Tomoyuki Nakagawa +7 more
doaj +3 more sources
Biosensor-Based Directed Evolution of Methanol Dehydrogenase from Lysinibacillus xylanilyticus. [PDF]
Int J Mol Sci, 2021 Methanol dehydrogenase (Mdh), is a crucial enzyme for utilizing methane and methanol as carbon and energy sources in methylotrophy and synthetic methylotrophy.
Le TK +8 more
europepmc +3 more sources
Methanol dehydrogenase from Hyphomicrobium MS 223 [PDF]
FEMS Microbiology Letters, 1985 The monomethyl sulfate-degrading bacterium, Hyphomicrobium MS 223, contains a NAD(P)-independent methanol dehydrogenase (EC 1.1.99.8) which was isolated and characterized. The enzyme was activated by ammonium ions, had an Mr of 118000 and was composed of two subunits of identical Mr.
Oreste Ghisalba, Hans-Peter Schär
exaly +2 more sources
Engineering serine metabolism to enhance AOX1 promoter self-induction in formate dehydrogenase-deficient Komagataella phaffii [PDF]
Microbial Cell FactoriesBackground The methylotrophic yeast Komagataella phaffii is a premier host for recombinant protein (rProt) production, which traditionally relies on methanol induction of the alcohol oxidase 1 promoter (P AOX1 ).
Rocio Cozmar +2 more
doaj +2 more sources
Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy [PDF]
Nature CommunicationsSynthetic methylotrophy offers opportunities for sustainable chemical and biofuel production. While recently established methylotrophic E. coli can grow on methanol, undesirable formate accumulation occurs during growth and bioproduction.
Philipp Keller +10 more
doaj +2 more sources
Biochemical and Structural Characterization of XoxG and XoxJ and Their Roles in Lanthanide‐Dependent Methanol Dehydrogenase Activity [PDF]
ChemBioChem, 2019 Emily R Featherston +2 more
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Oligotrophic Gene Expression in Rhodococcus erythropolis N9T-4 under Various Nutrient Conditions
Microorganisms, 2022 Rhodococcus erythropolis N9T-4 is a super oligotroph that grows on an inorganic basal medium without any additional carbon and nitrogen sources and requires CO2 for its oligotrophic growth. Previously, we found that two genes, aldA and mnoA, encoding NAD-
Yuri Ikeda +3 more
doaj +1 more source
Mechanism of methanol oxidation by quinoprotein methanol dehydrogenase [PDF]
Proceedings of the National Academy of Sciences, 2007 At neutral pH, oxidation of CH 3 OH → CH 2 O by an o -quinone requires general-base catalysis and the reaction is endothermic. The active-site –CO 2 − groups of Glu-171 and Asp-297 (Glu-171–CO 2 ...
Xiaodong, Zhang +2 more
openaire +2 more sources