Microorganisms metabolise methanol using either a methanol methyltransferase or a methanol dehydrogenase. Here, the authors use proteomics and stable isotope fractionation to show that a thermophilic sulfate-reducing bacterium, isolated from the deep ...
Diana Z. Sousa +10 more
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Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties. [PDF]
Bacillus methanolicus can utilize methanol as the sole carbon source for growth and it encodes an NAD(+)-dependent methanol dehydrogenase (Mdh), catalyzing the oxidation of methanol to formaldehyde. Recently, the genomes of the B.
Anne Krog +7 more
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Lanthanide-Dependent Regulation of Methylotrophy in
Methylobacterium species are representative of methylotrophic bacteria. Their genomes usually encode two types of methanol dehydrogenases (MDHs): MxaF and XoxF.
Sachiko Masuda +6 more
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Engineering Escherichia coli for Poly-β-hydroxybutyrate Production from Methanol
The naturally occurring one-carbon assimilation pathways for the production of acetyl-CoA and its derivatives often have low product yields because of carbon loss as CO2.
Jiaying Wang +4 more
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Lanthanide-dependent alcohol dehydrogenases have recently emerged as environmentally important enzymes, most prominently represented in methylotrophic bacteria.
Jing Huang +2 more
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The expanded diversity of methylophilaceae from Lake Washington through cultivation and genomic sequencing of novel ecotypes. [PDF]
We describe five novel Methylophilaceae ecotypes from a single ecological niche in Lake Washington, USA, and compare them to three previously described ecotypes, in terms of their phenotype and genome sequence divergence.
David A C Beck +9 more
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As an important metabolic enzyme in methylotrophs, pyrroloquinoline quinone (PQQ)-dependent alcohol dehydrogenases play significant roles in the global carbon and nitrogen cycles. In this article, a calcium (Ca2+)-dependent alcohol dehydrogenase PedE_M.s.
Ying Xiao +9 more
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Rare Earth Elements Alter Redox Balance in Methylomicrobium alcaliphilum 20ZR
Background: Rare Earth Elements (REEs) control methanol utilization in both methane- and methanol-utilizing microbes. It has been established that the addition of REEs leads to the transcriptional repression of MxaFI-MeDH [a two-subunit methanol ...
Ilya R. Akberdin +10 more
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Physiological Effect of XoxG(4) on Lanthanide-Dependent Methanotrophy
A recent surprising discovery of the activity of rare earth metals (lanthanides) as enzyme cofactors as well as transcriptional regulators has overturned the traditional assumption of biological inertia of these metals.
Yue Zheng +3 more
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Isolation of Facultative Methanol-utilizing Bacteria Which Produce Electrophoretically Different Methanol Dehydrogenase [PDF]
Bacterial strains capable of assimilating methanol as the sole source of carbon and energy have been isolated. These bacteria produced phenazine methosulfate-dependent methanol dehydrogenase (primary alcohol dehydrogenase, EC 1.1.99.8). The eilzyme activity was detected in electrophoresis on polyacrylamide gels by activity staining technique.
YAMANAKA, Kei, MATSUMOTO, Keisuke
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