Microbial Oxidation of Methane and Methanol: Crystallization of Methanol Dehydrogenase and Properties of Holo- and Apo-Methanol Dehydrogenase from Methylomonas methanica [PDF]
Journal of Bacteriology, 1978 Procedures are described for the purification and crystallization of methanol dehydrogenase from the soluble fraction of the type I obligate methylotroph Methylomonas methanica strain S1. The crystallized enzyme is homogeneous as judged by acrylamide gel electrophoresis and ultracentrifugation. The enzyme had a high
R N, Patel, C T, Hou, A, Felix
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Structure and Activity of the Prosthetic Group of Methanol Dehydrogenase [PDF]
European Journal of Biochemistry, 1980 The reconstitutive ability of the isolated prosthetic group of methanol dehydrogenase with the apoenzyme of glucose dehydrogenase and the results of electron spin resonance measurements suggest that the prosthetic group has not been modified during the isolation. This result, and the properties of the directly isolated prosthetic group and derivatives,
J A, Duine, J, Frank, P E, Verwiel
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"A Methylotrophic Thermotolerant Actinomycete With a NAD+- Dependent Methanol Dehydrogenase" [PDF]
Iranian Journal of Public Health, 2002 The methylotrophic thermo tolerant actinomycete strain 381, was isolated from a compost sample, assimilated methanol during growth. Methanol dehydrogenase could not be detected in cell extracts using dye-linked assays in the absence of NAD ...
S Eshraghi, Ian C Hancock, E Williams
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Novel methylotrophic bacteria isolated from the River Thames (London, UK) [PDF]
, 2008 Enrichment and elective culture for methylotrophs from sediment of the River Thames in central London yielded a diversity of pure cultures representing several genera of Gram-negative and Gram-positive bacteria, which were mainly of organisms not ...
Rich Boden +9 more
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Nature Communications, 2018 Microorganisms metabolise methanol using either a methanol methyltransferase or a methanol dehydrogenase. Here, the authors use proteomics and stable isotope fractionation to show that a thermophilic sulfate-reducing bacterium, isolated from the deep ...
Diana Z. Sousa +10 more
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Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD+ dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties. [PDF]
PLoS ONE, 2013 Bacillus methanolicus can utilize methanol as the sole carbon source for growth and it encodes an NAD(+)-dependent methanol dehydrogenase (Mdh), catalyzing the oxidation of methanol to formaldehyde. Recently, the genomes of the B.
Anne Krog +7 more
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Lanthanide-Dependent Regulation of Methylotrophy in
mSphere, 2018 Methylobacterium species are representative of methylotrophic bacteria. Their genomes usually encode two types of methanol dehydrogenases (MDHs): MxaF and XoxF.
Sachiko Masuda +6 more
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Engineering Escherichia coli for Poly-β-hydroxybutyrate Production from Methanol
Bioengineering, 2023 The naturally occurring one-carbon assimilation pathways for the production of acetyl-CoA and its derivatives often have low product yields because of carbon loss as CO2.
Jiaying Wang +4 more
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Isolation of Facultative Methanol-utilizing Bacteria Which Produce Electrophoretically Different Methanol Dehydrogenase [PDF]
Agricultural and Biological Chemistry, 1979 Bacterial strains capable of assimilating methanol as the sole source of carbon and energy have been isolated. These bacteria produced phenazine methosulfate-dependent methanol dehydrogenase (primary alcohol dehydrogenase, EC 1.1.99.8). The eilzyme activity was detected in electrophoresis on polyacrylamide gels by activity staining technique.
YAMANAKA, Kei, MATSUMOTO, Keisuke
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Frontiers in Microbiology, 2018 Lanthanide-dependent alcohol dehydrogenases have recently emerged as environmentally important enzymes, most prominently represented in methylotrophic bacteria.
Jing Huang +2 more
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