The expanded diversity of methylophilaceae from Lake Washington through cultivation and genomic sequencing of novel ecotypes. [PDF]
We describe five novel Methylophilaceae ecotypes from a single ecological niche in Lake Washington, USA, and compare them to three previously described ecotypes, in terms of their phenotype and genome sequence divergence.
David A C Beck +9 more
doaj +1 more source
Microbial oxidation of methane and methanol: crystallization and properties of methanol dehydrogenase from Methylosinus sporium [PDF]
Obligate methylotrophs are divisible into two types on the basis of ultrastructural biochemical characteristics. Both groups possess a soluble phenazine methosulfate (PMS)-dependent methanol dehydrogenase. In addition, particulate PMS-dependent methanol dehydrogenase and PMS-independent methanol oxidase have been found in the type I membrane group.
R N, Patel, A, Felix
openaire +2 more sources
Glycerol dehydrogenase: Structure, specificity, and mechanism of a family III polyol dehydrogenase
Background: Bacillus stearothermophilus glycerol dehydrogenase (GlyDH) (glycerol:NAD+ 2-oxidoreductase, EC 1.1.1.6) catalyzes the oxidation of glycerol to dihydroxyacetone (1,3-dihydroxypropanone) with concomitant reduction of NAD+ to NADH.
Baker PJ +26 more
core +1 more source
The interaction of methanol dehydrogenase and its cytochrome electron acceptor [PDF]
A fluorescence method is described for direct measurement of the interaction between methanol dehydrogenase (MDH) and its electron acceptor cytochrome cL. This has permitted a distinction to be made between factors affecting electron transfer and those affecting the initial binding or docking process.
S L, Dales, C, Anthony
openaire +2 more sources
Formate Formation and Formate Conversion in Biological Fuels Production
Biomethanation is a mature technology for fuel production. Fourth generation biofuels research will focus on sequestering CO2 and providing carbon-neutral or carbon-negative strategies to cope with dwindling fossil fuel supplies and environmental impact.
Crable, B.R. +7 more
core +1 more source
As an important metabolic enzyme in methylotrophs, pyrroloquinoline quinone (PQQ)-dependent alcohol dehydrogenases play significant roles in the global carbon and nitrogen cycles. In this article, a calcium (Ca2+)-dependent alcohol dehydrogenase PedE_M.s.
Ying Xiao +9 more
doaj +1 more source
Rare Earth Elements Alter Redox Balance in Methylomicrobium alcaliphilum 20ZR
Background: Rare Earth Elements (REEs) control methanol utilization in both methane- and methanol-utilizing microbes. It has been established that the addition of REEs leads to the transcriptional repression of MxaFI-MeDH [a two-subunit methanol ...
Ilya R. Akberdin +10 more
doaj +1 more source
Atypical one-carbon metabolism of an acetogenic and hydrogenogenic Moorella thermoacetica strain
A thermophilic spore-forming bacterium (strain AMP) was isolated from a thermophilic methanogenic bioreactor that was fed with cobalt-deprived synthetic medium containing methanol as substrate.
van Doesburg, W.C.J. +8 more
core +1 more source
Physiological Effect of XoxG(4) on Lanthanide-Dependent Methanotrophy
A recent surprising discovery of the activity of rare earth metals (lanthanides) as enzyme cofactors as well as transcriptional regulators has overturned the traditional assumption of biological inertia of these metals.
Yue Zheng +3 more
doaj +1 more source
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source

