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Xylose Utilisation: Cloning and Characterisation of the Xylose Reductase from Candida tenuis
Biological Chemistry, 1999Xylose reductases catalyse the initial reaction in the xylose utilisation pathway, the NAD(P)H+H+ dependent reduction of xylose to xylitol. In this work, the xylose reductase gene from Candida tenuis CBS 4435 was cloned and successfully expressed in E. coli. From the purified and partially sequenced protein primers were deduced for PCR.
B, Häcker +3 more
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Engineering yeasts for xylose metabolism
Current Opinion in Biotechnology, 2006Technologies for the production of alternative fuels are receiving increased attention owing to concerns over the rising cost of petrol and global warming. One such technology under development is the use of yeasts for the commercial fermentation of xylose to ethanol. Several approaches have been employed to engineer xylose metabolism.
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