Results 91 to 100 of about 11,026 (229)

Enzymatic Upgrading of Biomass‐Derived Aldoses to Rare Deoxy Ketoses Catalyzed by Transketolase Variants

ChemSusChem, Volume 18, Issue 8, April 14, 2025.
A sustainable, convenient, scalable, one‐step method for the two‐carbon chain elongation of cheap, biomass‐derived pentoses (l‐arabinose, and 2‐deoxy‐d‐ribose) and a hexose (l‐rhamnose) was developed to produce Cn+2 deoxy ketoses (C‐7 and C‐8) using transketolase, an enzyme catalyzing the irreversible transfer of a ketol group from an α‐ketoacid to an ...
Giuseppe Arbia, Muriel Joly, Lionel Nauton, Camilla Leogrande, Kai Tittmann, Franck Charmantray, Laurence Hecquet   +6 more
wiley   +1 more source

Directed enzyme evolution: climbing fitness peaks one amino acid at a time [PDF]

, 2009
Directed evolution can generate a remarkable range of new enzyme properties. Alternate substrate specificities and reaction selectivities are readily accessible in enzymes from families that are naturally functionally diverse.
Arnold, Frances H., Tracewell, Cara A.
core   +2 more sources

A Dive Into Yeast's Sugar Diet—Comparing the Metabolic Response of Glucose, Fructose, Sucrose, and Maltose Under Dynamic Feast/Famine Conditions

Biotechnology and Bioengineering, Volume 122, Issue 4, Page 1035-1050, April 2025.
ABSTRACT Microbes experience dynamic conditions in natural habitats as well as in engineered environments, such as large‐scale bioreactors, which exhibit increased mixing times and inhomogeneities. While single perturbations have been studied for several organisms and substrates, the impact of recurring short‐term perturbations remains largely unknown.
Koen Johannes Anthonius Verhagen, Ilse Henrike Pardijs, Hendrik Matthijs van Klaveren, Sebastian Aljoscha Wahl   +3 more
wiley   +1 more source

Engineering xylose metabolism in Thraustochytrid T18 [PDF]

, 2017
Thraustochytrids spp are oleaginous marine protists with significant potential for biofuel production at industrial levels; however, the cost of feedstocks has been a major challenge in making this process economical. On a quest for cheaper and renewable
Armenta, Roberto, Benjamin, Jeremy, Kottwitz, Haila, Merkx-Jacques, Alexandra, Muise, Denise, Rasmussen, Holly, Tanner, Kaitlyn, Woodhall, David   +7 more
core   +1 more source

Over-expression of Xylose isomerase/Xylulokinase and Construction of O2-tolerant hydrogenase in Klebsiella oxytoca HP1 [PDF]

, 2015
在石化燃料枯竭和全球气候变暖的双重危机下，氢气作为一种可再生、零排放和高热值的清洁能源载体而备受关注。关于氢能的研究主要围绕氢的制取和氢的利用两个方面展开。在众多制氢方式中，生物制氢因其生产成本低、原料来源丰富和环境污染小，从而成为制氢研究的热点。在氢的利用中，燃料电池技术一直以来都是研究的重点。氢酶是一种含金属的氧化还原酶，其广泛存在于微生物中，能够可逆地催化H+的还原和H2的氧化(2H++2e-↔H2)。无论是在氢的制取还是氢的利用中，氢酶都是氢能技术的核心之一 ...
黄钢锋
core  

How Does Nature Create the Painting “Gradient Coloration of ‘Manicure Finger’ Grape”? Integrated Omics Unveil the Pigments Basis and Metabolism Networks of Its Formation

Food Frontiers, Volume 6, Issue 2, Page 921-939, March 2025.
Based on integrated omics, the metabolic regulation networks underlying gradient coloration of ‘Manicure Finger’ grape were constructed at three levels of color‐presenting substances, structural genes, and transcription factors. ABSTRACT ‘Manicure Finger’ grape exhibits gradient coloration during veraison, where the lower part of the skin changes color
Shihan Bao, Jianing Li, Jiaqi Wang, Tian Lan, Mengyuan Wei, Xiangyu Sun, Yulin Fang, Tingting Ma   +7 more
wiley   +1 more source

Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose

Nature Communications
To broaden the substrate scope of microbial cell factories towards renewable substrates, rational genetic interventions are often combined with adaptive laboratory evolution (ALE).
Pavel Dvořák, Barbora Burýšková, Barbora Popelářová, Birgitta E. Ebert, Tibor Botka, Dalimil Bujdoš, Alberto Sánchez-Pascuala, Hannah Schöttler, Heiko Hayen, Víctor de Lorenzo, Lars M. Blank, Martin Benešík   +11 more
doaj   +1 more source

Cross-linked protein crystal technology in bioseparation and biocatalytic applications [PDF]

, 2004
Chemical cross-linking of protein crystals form an insoluble and active protein matrix. Cross-linked protein crystals (CLPCs) have many excellent properties including high volumetric activity and stability.
Vuolanto, Antti
core  

Enhanced whole genome sequence and annotation of Clostridium stercorarium DSM8532T using RNA-seq transcriptomics and high-throughput proteomics [PDF]

, 2014
BACKGROUND: Growing interest in cellulolytic clostridia with potential for consolidated biofuels production is mitigated by low conversion of raw substrates to desired end products.
Alvare, Graham, Fristensky, Brian, Henrissat, Bernard, Krokhin, Oleg, Levin, David, McQueen, Peter, Schellenberg, John, Sparling, Richard, Thallinger, Gerhard, Verbeke, Tobin, Wilkins, John, Zhang, Xiangli   +11 more
core   +2 more sources

Metabolic insights of lactic acid bacteria in reducing off‐flavors and antinutrients in plant‐based fermented dairy alternatives

Comprehensive Reviews in Food Science and Food Safety, Volume 24, Issue 2, March 2025.
Abstract Multiple sensorial, technological, and nutritional challenges must be overcome when developing plant‐based fermented dairy alternatives (PBFDA) to mimic their dairy counterparts. The elimination of plant‐derived off‐flavors (green, earthy, bitter, astringent) and the degradation of antinutrients are crucial quality factors highlighted by the ...
Guillermo Eduardo Sedó Molina, Geoffrey Ras, Denise Felix da Silva, Lene Duedahl‐Olesen, Egon Bech Hansen, Claus Heiner Bang‐Berthelsen   +5 more
wiley   +1 more source