This study engineers Yarrowia lipolytica to produce succinic acid (SA) from lignocellulosic biomass. Adaptive evolution and multi‐omics analysis identify regulators that alleviate xylose metabolic disorder. Multi‐copy integration of xylose metabolic genes resolves redox imbalances from a futile fatty acid cycle, producing high‐titer SA from corn stover
Yutao Zhong+7 more
openalex +2 more sources
Engineering of a Xylose Metabolic Pathway in Rhodococcus Strains [PDF]
ABSTRACTThe two metabolically versatile actinobacteriaRhodococcus opacusPD630 andR. jostiiRHA1 can efficiently convert diverse organic substrates into neutral lipids mainly consisting of triacylglycerol (TAG), the precursor of energy-rich hydrocarbon.
Xiong, Xiaochao, Wang, Xi, Chen, Shulin
openaire +4 more sources
Novel strategies to improve co-fermentation of pentoses with D-glucose by recombinant yeast strains in lignocellulosic hydrolysates [PDF]
Economically feasible production of second-generation biofuels requires efficient co-fermentation of pentose and hexose sugars in lignocellulosic hydrolysates under very harsh conditions. Baker’s yeast is an excellent, traditionally used ethanol producer
Boles, Eckhard+3 more
core +1 more source
Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus. [PDF]
Prefoldin (PFD) is a group II chaperonin that is ubiquitously present in the eukaryotic kingdom. Six subunits (PFD1-6) form a jellyfish-like heterohexameric PFD complex and function in protein folding and cytoskeleton organization.
Barry, Kerrie+20 more
core +1 more source
RNAseq reveals hydrophobins that are involved in the adaptation of aspergillus nidulans to lignocellulose [PDF]
Background Sugarcane is one of the world’s most profitable crops. Waste steam-exploded sugarcane bagasse (SEB) is a cheap, abundant, and renewable lignocellulosic feedstock for the next-generation biofuels.
Brown, Neil Andrew+7 more
core +4 more sources
Development of a D-xylose fermenting and inhibitor tolerant industrial Saccharomyces cerevisiae strain with high performance in lignocellulose hydrolysates using metabolic and evolutionary engineering [PDF]
Background: The production of bioethanol from lignocellulose hydrolysates requires a robust, D-xylose-fermenting and inhibitor-tolerant microorganism as catalyst.
Boles, Eckhard+13 more
core +1 more source
Complete genome sequence of Halorhabdus utahensis type strain (AX-2). [PDF]
Halorhabdus utahensis Wainø et al. 2000 is the type species of the genus, which is of phylogenetic interest because of its location on one of the deepest branches within the very extensive euryarchaeal family Halobacteriaceae. H.
Anderson, Iain+36 more
core +2 more sources
Analysis of a conserved cellulase transcriptional regulator reveals inducer-independent production of cellulolytic enzymes in Neurospora crassa. [PDF]
Cellulose is recalcitrant to deconstruction to glucose for use in fermentation strategies for biofuels and chemicals derived from lignocellulose. In Neurospora crassa, the transcriptional regulator, CLR-2, is required for cellulolytic gene expression and
Coradetti, Samuel+2 more
core +2 more sources
Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls [PDF]
Arabidopsis cell walls contain large amounts of pectins and hemicelluloses, which are predominantly synthesized via the common precursor UDP-glucuronic acid.
Klinghammer, Michaela, Tenhaken, Raimund
core +1 more source
16S rRNA gene profiling and genome reconstruction reveal community metabolic interactions and prebiotic potential of medicinal herbs used in neurodegenerative disease and as nootropics. [PDF]
The prebiotic potential of nervine herbal medicines has been scarcely studied. We therefore used anaerobic human fecal cultivation to investigate whether medicinal herbs commonly used as treatment in neurological health and disease in Ayurveda and other ...
Albayrak, Levent+10 more
core +5 more sources