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Metabolic Control Analysis of Xylose Catabolism in Aspergillus
Biotechnology Progress, 2008A kinetic model for xylose catabolism in Aspergillus is proposed. From a thermodynamic analysis it was found that the intermediate xylitol will accumulate during xylose catabolism. Use of the kinetic model allowed metabolic control analysis (MCA) of the xylose catabolic pathway to be carried out, and flux control was shown to be dependent on the ...
Prathumpai, W. +6 more
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Influence of glucose on xylose metabolization by Spathaspora passalidarum
Fungal Genetics and Biology, 2021The yeast Spathaspora passalidarum is able to produce ethanol from D-xylose and D-glucose. However, it is not clear how xylose metabolism is affected by D-glucose when both sugars are available in the culture medium. The aims of this work were to evaluate the influence of D-glucose on D-xylose consumption, ethanol production, gene expression, and the ...
Lílian E, Ribeiro +10 more
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D-Xylose metabolism in Rhodosporidium toruloides
Biotechnology Letters, 1997Hofer et al. (Biochem. Biophys. Acta 1971. 252:1-12) presented circumstantial evidence that suggested that Rhodosporidium toruloides produced a xylose isomerase. We were unable to detect this activity in cell-free extracts of this yeast, however, xylose reductase and xylitol dehydrogenase activities were detected.
S.N. Freer, C.D. Skory, R.J. Bothast
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Engineering yeasts for xylose metabolism
Current Opinion in Biotechnology, 2006Technologies for the production of alternative fuels are receiving increased attention owing to concerns over the rising cost of petrol and global warming. One such technology under development is the use of yeasts for the commercial fermentation of xylose to ethanol. Several approaches have been employed to engineer xylose metabolism.
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Xylose metabolism in genetic variants of Salmonella typhosa
Archives of Biochemistry and Biophysics, 1957Abstract Evidence has been presented which establishes steps in the route of xylose utilization in certain strains of S. typhosa . The failure of the negative strain to metabolize d -xylose stems from its inability to form the enzyme, xylose isomerase, and the enzyme, xylulokinase. The positive strain of S.
L S, BARON, E S, KLINE
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Metabolic pathway analysis of the xylose-metabolizing yeast protoplast fusant ZLYRHZ7
Journal of Bioscience and Bioengineering, 2017Xylose is the second major fermentable sugar present in hard woods and herbs (after d-glucose). Therefore, efficient conversion of xylose to ethanol is essential for the commercialization of lignocellulosic ethanol, which may provide an ideal alternative to fossil fuels in the future.
Jingping, Ge +4 more
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Journal of Biotechnology, 2012
Efficient conversion of xylose to ethanol is an essential factor for commercialization of lignocellulosic ethanol. To minimize production of xylitol, a major by-product in xylose metabolism and concomitantly improve ethanol production, Saccharomyces cerevisiae D452-2 was engineered to overexpress NADH-preferable xylose reductase mutant (XR(MUT)) and ...
Sung-Haeng, Lee +3 more
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Efficient conversion of xylose to ethanol is an essential factor for commercialization of lignocellulosic ethanol. To minimize production of xylitol, a major by-product in xylose metabolism and concomitantly improve ethanol production, Saccharomyces cerevisiae D452-2 was engineered to overexpress NADH-preferable xylose reductase mutant (XR(MUT)) and ...
Sung-Haeng, Lee +3 more
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Metabolic Engineering of Saccharomyces cerevisiae for Xylose Utilization
2001Metabolic engineering of Saccharomyces cerevisiae for ethanolic fermentation of xylose is summarized with emphasis on progress made during the last decade. Advances in xylose transport, initial xylose metabolism, selection of host strains, transformation and classical breeding techniques applied to industrial polyploid strains as well as modeling of ...
B, Hahn-Hägerdal +5 more
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Journal of Biotechnology, 2010
Xylose-fermenting Saccharomyces strains are needed for commercialization of ethanol production from lignocellulosic biomass. Engineered Saccharomyces cerevisiae strains expressing XYL1, XYL2 and XYL3 from Pichia stipitis, however, utilize xylose in an oxidative manner, which results in significantly lower ethanol yields from xylose as compared to ...
Soo Rin, Kim +6 more
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Xylose-fermenting Saccharomyces strains are needed for commercialization of ethanol production from lignocellulosic biomass. Engineered Saccharomyces cerevisiae strains expressing XYL1, XYL2 and XYL3 from Pichia stipitis, however, utilize xylose in an oxidative manner, which results in significantly lower ethanol yields from xylose as compared to ...
Soo Rin, Kim +6 more
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Xylose metabolism by Pichia stipitis: the effect of ethanol
Applied Microbiology and Biotechnology, 1991Pichia stipitis Y7124 was grown anaerobically on d-xylose in the presence of an initial ethanol concentration (E0) varying from 0 to 40 g/l. When E0 increased, the yield of xylitol increased linearly, reaching a value of 0.20 mol xylitol/mol xylose at E0=40 g/l.
Delgenès, Jean-Philippe +2 more
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