Results 161 to 170 of about 7,576 (203)
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An assay for functional xylose transporters in Saccharomyces cerevisiae
Analytical Biochemistry, 2013It has been considered that more efficient uptake of xylose could promote increased xylose metabolic capacity of several microorganisms. In this study, an assay to screen xylose transporters was established in the Saccharomyces cerevisiae strain, which expresses the xylosidase gene of Bacillus pumilus intracellularly.
Chengqiang, Wang +4 more
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D-Xylose Transport by Candida succiphila and Kluyveromyces marxianus
Applied Biochemistry and Biotechnology, 2003The kinetics and regulation of D-xylose uptake were investigated in the efficient pentose fermentor Candida succiphila, and in Kluyveromyces marxianus, which assimilate but do not ferment pentose sugars. Active high affinity (Km approximately 3.8 mM; Vmax approximately 15 nmol/[mg min]) and putative facilitated diffusion low-affinity (Km approximately ...
Boris U, Stambuk +3 more
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Current Microbiology, 1998
Thermoanaerobacter ethanolicus is a xylose-utilizing thermophilic anaerobe that produces considerable amounts of ethanol. A protein in xylose-growing cells was solubilized from cell membranes by extraction with octyl-beta-glucoside. Internal peptide sequencing revealed that the protein was the product of a gene, xylF, encoding a putative D-xylose ...
, Erbeznik, , Ray, , Dawson, , Strobel
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Thermoanaerobacter ethanolicus is a xylose-utilizing thermophilic anaerobe that produces considerable amounts of ethanol. A protein in xylose-growing cells was solubilized from cell membranes by extraction with octyl-beta-glucoside. Internal peptide sequencing revealed that the protein was the product of a gene, xylF, encoding a putative D-xylose ...
, Erbeznik, , Ray, , Dawson, , Strobel
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Mechanism of D-Xylose Transport in Human Small Intestine
Journal of Pediatric Gastroenterology and Nutrition, 1986Summary: The transport mechanism of D‐xylose across the mucosal border of small intestine was investigated in humans both in vitro and in vivo. A comparative study was also made between infants and adults. We investigated the Na+ dependence of D‐xylose influx from the mucosal solution into the epithelial cells, the phlorizin sensitivity of the influx ...
N, Ohkohchi +3 more
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Biotechnology Journal, 2021
AbstractSimultaneous co‐fermentation of glucose and xylose is a key desired trait of engineered Saccharomyces cerevisiae for efficient and rapid production of biofuels and chemicals. However, glucose strongly inhibits xylose transport by endogenous hexose transporters of S. cerevisiae.
Nurzhan Kuanyshev +8 more
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AbstractSimultaneous co‐fermentation of glucose and xylose is a key desired trait of engineered Saccharomyces cerevisiae for efficient and rapid production of biofuels and chemicals. However, glucose strongly inhibits xylose transport by endogenous hexose transporters of S. cerevisiae.
Nurzhan Kuanyshev +8 more
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Kinetic studies on glucose and xylose transport in Saccharomyces cerevisiae
Applied Microbiology and Biotechnology, 2002Zero trans-influx assays of glucose and xylose were performed using Saccharomyces cerevisiae to investigate transport characteristics under high and low glucose conditions. Under high glucose conditions, most glucose was transported by the low-affinity transporter.
J -H Seo
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Yeast, 2004
AbstractWe have developed recombinant Saccharomyces yeasts that can effectively co‐ferment glucose and xylose to ethanol. However, these yeasts still ferment glucose more efficiently than xylose. The transport of xylose could be one of the steps limiting the fermentation of xylose.
Miroslav, Sedlak, Nancy W Y, Ho
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AbstractWe have developed recombinant Saccharomyces yeasts that can effectively co‐ferment glucose and xylose to ethanol. However, these yeasts still ferment glucose more efficiently than xylose. The transport of xylose could be one of the steps limiting the fermentation of xylose.
Miroslav, Sedlak, Nancy W Y, Ho
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Applied Microbiology and Biotechnology, 2007
In the present study, we modified xylose uptake properties of a recombinant xylose-utilizing yeast Saccharomyces cerevisiae by expression of heterologous and homologous permease-encoding genes. In a mutant yeast strain with the main seven hexose transporter genes deleted, and engineered for xylose utilization, we screened an expression cDNA library of ...
Oleh V Stasyk +2 more
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In the present study, we modified xylose uptake properties of a recombinant xylose-utilizing yeast Saccharomyces cerevisiae by expression of heterologous and homologous permease-encoding genes. In a mutant yeast strain with the main seven hexose transporter genes deleted, and engineered for xylose utilization, we screened an expression cDNA library of ...
Oleh V Stasyk +2 more
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A kinetic analysis of d-xylose transport in rhodotorula glutinis
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1978The kinetics of D-xylose transport were studied in Rhodotorula glutinis. Analysis of the saturation isotherm revealed the presence of at least two carriers for D-xylose in the Rhodotorula plasma membrane. These two carriers exhibited Km values differing by more than an order of magnitude.
M E, Alcorn, C C, Griffin
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Understanding xylose transport in yeasts
Xylose constitutes the second major sugar fraction of the plant-derived lignocellulosic biomass, which is the most abundantly available and renewable feedstock for microbial fermentations. Hence, comprehensive utilization of xylose is crucial from the perspective of sustainable development of bio-based products, such as fuels, fine chemicals, and high ...Atrayee Chattopadhyay +2 more
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