Results 191 to 200 of about 15,217 (230)

Control of xylose consumption by xylose transport in recombinant Saccharomyces cerevisiae

Biotechnology and Bioengineering, 2003
AbstractSaccharomyces cerevisiae TMB3001 has previously been engineered to utilize xylose by integrating the genes coding for xylose reductase (XR) and xylitol dehydrogenase (XDH) and overexpressing the native xylulokinase (XK) gene. The resulting strain is able to metabolize xylose, but its xylose utilization rate is low compared to that of natural ...
Márk, Gárdonyi, Marie, Jeppsson, Gunnar, Lidén, Marie F, Gorwa-Grauslund, Bärbel, Hahn-Hägerdal   +4 more
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Directed evolution of xylose specific transporters to facilitate glucose‐xylose co‐utilization

Biotechnology and Bioengineering, 2015
ABSTRACTA highly active xylose specific transporter without glucose inhibition is highly desirable in cost‐effective production of biofuels from lignocellulosic biomass. However, currently available xylose specific transporters suffer from low overall activity and most are inhibited by glucose. In this study, we applied a directed evolution strategy to
Meng, Wang, Chenzhao, Yu, Huimin, Zhao
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Transport of xylose and glucose in the xylose-fermenting yeast Pichia stipitis

Applied Microbiology and Biotechnology, 1988
A low-affinity and a high-affinity sylose proton symport operated simultaneously in both starved and non-starved cells of Pichia stipitis. Glucose competed with xylose for transport by the low-affinity system and inhibited xylose transport by the high-affinity system non-competitively.
S. G. Kilian, N. van Uden
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Expression of a heterologous xylose transporter in a Saccharomyces cerevisiae strain engineered to utilize xylose improves aerobic xylose consumption

Applied Microbiology and Biotechnology, 2008
The goal of this investigation was to determine the effect of a xylose transport system on glucose and xylose co-consumption as well as total xylose consumption in Saccharomyces cerevisiae. We expressed two heterologous transporters from Arabidopsis thaliana in recombinant xylose-utilizing S. cerevisiae cells.
Ronald E, Hector, Nasib, Qureshi, Stephen R, Hughes, Michael A, Cotta   +3 more
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D-Xylose Transport by Candida succiphila and Kluyveromyces marxianus

Applied Biochemistry and Biotechnology, 2003
The kinetics and regulation of D-xylose uptake were investigated in the efficient pentose fermentor Candida succiphila, and in Kluyveromyces marxianus, which assimilate but do not ferment pentose sugars. Active high affinity (Km approximately 3.8 mM; Vmax approximately 15 nmol/[mg min]) and putative facilitated diffusion low-affinity (Km approximately ...
Boris U, Stambuk, Mary Ann, Franden, Arjun, Singh, Min, Zhang   +3 more
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Mechanism of D-Xylose Transport in Human Small Intestine

Journal of Pediatric Gastroenterology and Nutrition, 1986
Summary: The transport mechanism of D‐xylose across the mucosal border of small intestine was investigated in humans both in vitro and in vivo. A comparative study was also made between infants and adults. We investigated the Na+ dependence of D‐xylose influx from the mucosal solution into the epithelial cells, the phlorizin sensitivity of the influx ...
N, Ohkohchi, M, Himukai, Y, Igarashi, M, Kasai   +3 more
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Understanding xylose transport in yeasts

Xylose constitutes the second major sugar fraction of the plant-derived lignocellulosic biomass, which is the most abundantly available and renewable feedstock for microbial fermentations. Hence, comprehensive utilization of xylose is crucial from the perspective of sustainable development of bio-based products, such as fuels, fine chemicals, and high ...
Atrayee Chattopadhyay, Mohor Mitra, Mrinal K. Maiti   +2 more
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Xylose and xylose/glucose co-fermentation by recombinant Saccharomyces cerevisiae strains expressing individual hexose transporters

Enzyme and Microbial Technology, 2014
Since the uptake of xylose is believed to be one of the rate-limiting steps for xylose ethanol fermentation by recombinant Saccharomyces cerevisiae strains, we transformed a hxt-null strain lacking the major hexose transporters (hxt1Δ-hxt7Δ and gal2Δ) with an integrative plasmid to overexpress the genes for xylose reductase (XYL1), xylitol ...
Davi L, Gonçalves, Akinori, Matsushika, Belisa B, de Sales, Tetsuya, Goshima, Elba P S, Bon, Boris U, Stambuk   +5 more
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An assay for functional xylose transporters in Saccharomyces cerevisiae

Analytical Biochemistry, 2013
It has been considered that more efficient uptake of xylose could promote increased xylose metabolic capacity of several microorganisms. In this study, an assay to screen xylose transporters was established in the Saccharomyces cerevisiae strain, which expresses the xylosidase gene of Bacillus pumilus intracellularly.
Chengqiang, Wang, Yu, Shen, Jin, Hou, Fan, Suo, Xiaoming, Bao   +4 more
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Identification and analysis of sugar transporters capable of co‐transporting glucose and xylose simultaneously

Biotechnology Journal, 2021
AbstractSimultaneous co‐fermentation of glucose and xylose is a key desired trait of engineered Saccharomyces cerevisiae for efficient and rapid production of biofuels and chemicals. However, glucose strongly inhibits xylose transport by endogenous hexose transporters of S. cerevisiae.
Nurzhan Kuanyshev, Anshu Deewan, Sujit Sadashiv Jagtap, Jingjing Liu, Balaji Selvam, Li‐Qing Chen, Diwakar Shukla, Christopher V. Rao, Yong‐Su Jin   +8 more
openaire   +3 more sources