Results 201 to 210 of about 169,452 (247)

Expression of a heterologous xylose transporter in a Saccharomyces cerevisiae strain engineered to utilize xylose improves aerobic xylose consumption

Applied Microbiology and Biotechnology, 2008
The goal of this investigation was to determine the effect of a xylose transport system on glucose and xylose co-consumption as well as total xylose consumption in Saccharomyces cerevisiae. We expressed two heterologous transporters from Arabidopsis thaliana in recombinant xylose-utilizing S. cerevisiae cells.
Nasib Qureshi, Ronald Hector, Michael Cotta   +2 more
exaly   +3 more sources

Xylose transport pathways in rabbit ileum

American Journal of Physiology-Gastrointestinal and Liver Physiology, 1980
The D-xylose transepithelial transport mechanism was examined in rabbit ileum in vitro. The net flux of D-xylose (Jxylnet) from mucosa to serosa is abolished in the presence of 10(-4) M ouabain. D-Xylose stimulates the net flux of sodium (JNanet) and the short-circuit current (Isc) equally.
M, Heyman, A M, Dumontier, J F, Desjeux
openaire   +2 more sources

Control of xylose consumption by xylose transport in recombinant Saccharomyces cerevisiae

Biotechnology and Bioengineering, 2003
AbstractSaccharomyces cerevisiae TMB3001 has previously been engineered to utilize xylose by integrating the genes coding for xylose reductase (XR) and xylitol dehydrogenase (XDH) and overexpressing the native xylulokinase (XK) gene. The resulting strain is able to metabolize xylose, but its xylose utilization rate is low compared to that of natural ...
Márk, Gárdonyi, Marie, Jeppsson, Gunnar, Lidén, Marie F, Gorwa-Grauslund, Bärbel, Hahn-Hägerdal   +4 more
openaire   +2 more sources

Transport of xylose and glucose in the xylose-fermenting yeast Pichia stipitis

Applied Microbiology and Biotechnology, 1988
A low-affinity and a high-affinity sylose proton symport operated simultaneously in both starved and non-starved cells of Pichia stipitis. Glucose competed with xylose for transport by the low-affinity system and inhibited xylose transport by the high-affinity system non-competitively.
S. G. Kilian, N. van Uden
openaire   +1 more source

Engineering and adaptive evolution of Escherichia coli for d-lactate fermentation reveals GatC as a xylose transporter

Metabolic Engineering, 2012
Despite the abundance of xylose in nature, the production of chemicals from C5 sugars remains challenging in metabolic engineering. By deleting xylFGH genes and using adaptive evolution, an efficient E.
Lars Keld Nielsen, Cuauhtémoc Licona-Cassani, Alfredo Martinez   +2 more
exaly   +2 more sources

Directed evolution of xylose specific transporters to facilitate glucose‐xylose co‐utilization

Biotechnology and Bioengineering, 2015
ABSTRACTA highly active xylose specific transporter without glucose inhibition is highly desirable in cost‐effective production of biofuels from lignocellulosic biomass. However, currently available xylose specific transporters suffer from low overall activity and most are inhibited by glucose. In this study, we applied a directed evolution strategy to
Meng, Wang, Chenzhao, Yu, Huimin, Zhao
openaire   +2 more sources

Molecular Modification Enhances Xylose Uptake by the Sugar Transporter KM_SUT5 of Kluyveromyces marxianus

International Journal of Molecular Sciences
This research cloned and expressed the sugar transporter gene KM_SUT5 from Kluyveromyces marxianus GX-UN120, which displayed remarkable sugar transportation capabilities, including pentose sugars.
Xiuyuan Luo
exaly   +2 more sources

An assay for functional xylose transporters in Saccharomyces cerevisiae

Analytical Biochemistry, 2013
It has been considered that more efficient uptake of xylose could promote increased xylose metabolic capacity of several microorganisms. In this study, an assay to screen xylose transporters was established in the Saccharomyces cerevisiae strain, which expresses the xylosidase gene of Bacillus pumilus intracellularly.
Chengqiang, Wang, Yu, Shen, Jin, Hou, Fan, Suo, Xiaoming, Bao   +4 more
openaire   +2 more sources

D-Xylose Transport by Candida succiphila and Kluyveromyces marxianus

Applied Biochemistry and Biotechnology, 2003
The kinetics and regulation of D-xylose uptake were investigated in the efficient pentose fermentor Candida succiphila, and in Kluyveromyces marxianus, which assimilate but do not ferment pentose sugars. Active high affinity (Km approximately 3.8 mM; Vmax approximately 15 nmol/[mg min]) and putative facilitated diffusion low-affinity (Km approximately ...
Boris U, Stambuk, Mary Ann, Franden, Arjun, Singh, Min, Zhang   +3 more
openaire   +2 more sources

Mechanism of D-Xylose Transport in Human Small Intestine

Journal of Pediatric Gastroenterology and Nutrition, 1986
Summary: The transport mechanism of D‐xylose across the mucosal border of small intestine was investigated in humans both in vitro and in vivo. A comparative study was also made between infants and adults. We investigated the Na+ dependence of D‐xylose influx from the mucosal solution into the epithelial cells, the phlorizin sensitivity of the influx ...
N, Ohkohchi, M, Himukai, Y, Igarashi, M, Kasai   +3 more
openaire   +2 more sources