Results 61 to 70 of about 15,217 (230)

Asking the 5 W's for designing next‐generation bioprocessing

AIChE Journal, EarlyView.
Abstract Biotechnology is expanding beyond traditional, centralized fermentation and toward next‐generation bioprocessing paradigms that emphasize flexible deployment outside the laboratory with application‐specific performance. However, many bioprocesses fail to translate beyond proof‐of‐concept into industrially viable systems because early design ...
Sangdo Yook, Karoline Maria Vieira Nogueira, Raj Sukumar, Jenna Miller, Hal S. Alper   +4 more
wiley   +1 more source

Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant Candida tropicalis

Energies, 2022
Candida tropicalis, a xylose-fermenting yeast, has the potential for converting cellulosic biomass to ethanol. Thermotolerant C. tropicalis X-17, which was isolated in Laos, was subjected to repetitive long-term cultivation with a gradual increase in ...
Koudkeo Phommachan, Chansom Keo-oudone, Mochamad Nurcholis, Nookhao Vongvilaisak, Mingkhuan Chanhming, Vanhnavong Savanhnaly, Somchanh Bounphanmy, Minenosuke Matsutani, Tomoyuki Kosaka, Savitree Limtong, Mamoru Yamada   +10 more
doaj   +1 more source

Mechanism of imidazolium ionic liquids toxicity in Saccharomyces cerevisiae and rational engineering of a tolerant, xylose-fermenting strain [PDF]

, 2016
Additional file 3. Fermentation profiles of Y133 and Y133-IIL in the presence of 1 % [BMIM]Cl at pH 6.5 and pH 5.0, and either aerobic or anaerobic conditions (n = 3, Mean ± S.E, except n = 2 for Y133 pH 6.5 anaerobic 72 h)
Alexander Hebert, Chad L. Myers, Charles Boone, Jeff S. Piotrowski, Joshua J. Coon, Li Hinchman, Quinn Dickinson, Robert Landick, Scott Bottoms, Sean McIlwain, Sheena Li, Trey K. Sato   +11 more
core   +5 more sources

All You Can Eat Yeast: Substituting Hexose Transporters With AtSWEET7 Alleviates Glucose Repression, Enabling Simultaneous Utilization of Sugars in Renewable Feedstocks

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Yeast sugar transporters have highly evolved for preferential glucose transport, a significant roadblock for utilizing non‐glucose sugars in renewable feedstocks such as lignocellulosic biomass. To enable simultaneous transport of multiple sugars, native hexose transporters were replaced by SWEET7p from Arabidopsis thaliana in engineered ...
Nurzhan Kuanyshev, Degaulle Dai, Jungyeon Kim, Nam Kyu Kang, Ming‐Hsun Cheng, Vijay Singh, Yong‐Su Jin   +6 more
wiley   +1 more source

Advancing Malic Acid Production of Aspergillus oryzae: Combined Process Optimization and Application of Various Substrates

Biotechnology and Bioengineering, EarlyView.
ABSTRACT In light of climate change and growing resource scarcity, microbial production of organic acids offers a sustainable alternative to fossil‐based chemical synthesis. In this study, malic acid production by Aspergillus oryzae was optimized through cultivation temperature adjustment and biotin supplementation, while organic acid formation from ...
Lukas Hartmann, Anke Neumann, Dirk Holtmann, Björn Vater, Thomas Hahn, Susanne Zibek, Katrin Ochsenreither   +6 more
wiley   +1 more source

Insights from the fungus Fusarium oxysporum point to high affinity glucose transporters as targets for enhancing ethanol production from lignocellulose. [PDF]

PLoS ONE, 2013
Ethanol is the most-widely used biofuel in the world today. Lignocellulosic plant biomass derived from agricultural residue can be converted to ethanol via microbial bioprocessing.
Shahin S Ali, Brian Nugent, Ewen Mullins, Fiona M Doohan   +3 more
doaj   +1 more source

Strategies for Efficient Expression of Heterologous Monosaccharide Transporters in Saccharomyces cerevisiae

Journal of Fungi, 2022
In previous work, we developed a Saccharomyces cerevisiae strain (DLG-K1) lacking the main monosaccharide transporters (hxt-null) and displaying high xylose reductase, xylitol dehydrogenase and xylulokinase activities.
Marilia M. Knychala, Angela A. dos Santos, Leonardo G. Kretzer, Fernanda Gelsleichter, Maria José Leandro, César Fonseca, Boris U. Stambuk   +6 more
doaj   +1 more source

Elucidation of the recognition mechanisms for hemicellulose and pectin in Clostridium cellulovorans using intracellular quantitative proteome analysis [PDF]

, 2015
Clostridium cellulovorans is an anaerobic, cellulolytic bacterium, capable of effectively degrading and metabolizing various types of substrates, including cellulose, hemicellulose (xylan and galactomannan), and pectin.
Hironobu Morisaka, Kohei Esaka, Kouichi Kuroda, Mitsuyoshi Ueda, Shunsuke Aburaya   +4 more
core   +2 more sources

The power of many: when genetics met yeasts and high‐throughput

Biological Reviews, EarlyView.
ABSTRACT In recent years, complex technological capabilities have evolved, driven by the need to solve complex and integrative biological questions through global analyses. New equipment allows the scaling up and automation of processes which previously were carried out on a very limited scale.
Víctor A. Tallada, Víctor Carranco
wiley   +1 more source

The Penicillium chrysogenum transporter PcAraT enables high-affinity, glucose-insensitive l-arabinose transport in Saccharomyces cerevisiae

Biotechnology for Biofuels, 2018
Background l-Arabinose occurs at economically relevant levels in lignocellulosic hydrolysates. Its low-affinity uptake via the Saccharomyces cerevisiae Gal2 galactose transporter is inhibited by d-glucose. Especially at low concentrations of l-arabinose,
Jasmine M. Bracher, Maarten D. Verhoeven, H. Wouter Wisselink, Barbara Crimi, Jeroen G. Nijland, Arnold J. M. Driessen, Paul Klaassen, Antonius J. A. van Maris, Jean-Marc G. Daran, Jack T. Pronk   +9 more
doaj   +1 more source