Production of antibacterial compounds by a Steely hybrid polyketide synthase in Dictyostelium
By selecting appropriate production species under optimised culture conditions, new chlorinated dibenzofuran compounds, which previously could not be analysed in sufficient quantities, were identified from cellular slime mould fruiting bodies. The strong antibacterial activity exhibited by these compounds suggests a link to the ecological function of ...
Tomoaki R. Yamashita +3 more
wiley +1 more source
Targeted Mutagenesis of the Oligopeptide Repeat Domain of the Yeast Prion Sup35 [PDF]
The formation of prions in the baker’s yeast Saccharomyces cerevisiae is determined by amino acid composition rather than the primary sequence of amino acids.
Davis, Emily, Knox, James D.
core +1 more source
Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification [PDF]
Alu-PCR protocols were optimized for the generation of human DNA probes from yeast strains containing yeast artificial chromosomes (YACs) with human inserts between 100 and 800 kb in size.
Brooks-Wilson +15 more
core +1 more source
Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
The aim of this study was to enhance the nitrogen supply through three different mash treatments and to investigate their effects on fermentation dynamics, yeast biomass accumulation, and the concentration of aroma-active volatiles in nitrogen-poor apple
Ana Schön +5 more
doaj +1 more source
Distinct functional relevance of dynamic GTPase cysteine methylation in fission yeast
The final step in post-translational processing of Ras and Rho GTPases involves methylation of the prenylated cysteine residue by an isoprenylcysteine-O-carboxyl methyltransferase (ICMT).
Alejandro Franco +9 more
doaj +1 more source
Marine yeast isolation and industrial application [PDF]
Over the last century, terrestrial yeasts have been widely used in various industries, such as baking, brewing, wine, bioethanol and pharmaceutical protein production. However, only little attention has been given to marine yeasts. Recent research showed
Abdelrahman Saleh Zaky +93 more
core +1 more source
Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Preparation of Wine from Jackfruit (Artocarpus heterophyllus lam)Juice Using Baker yeast: Effect of Yeast and Initial Sugar Concentrations [PDF]
The overproduction of jackfruit (Artocarpus heterophyllus Lam) during harvest season and its short shelf-life have caused serious losses for farmers.
Budiyati, C.S. +4 more
core
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source

