Results 301 to 310 of about 1,336,378 (359)

High‐sensitivity real‐time monitoring of pH and respiration activity unveils metabolic dynamics in shake flask cultures

Biotechnology Progress, Volume 41, Issue 2, March/April 2025.
Abstract Erlenmeyer shake flasks are widely used during the first steps of bioprocess development. Despite their broad application in academia and industry, shake flasks usually lack standardized and user‐friendly online monitoring techniques. In this work, the pH and Respiratory Activity MOnitoring System (pH‐RAMOS) for the non‐invasive online ...
Burak Sarikaya, Karsten Günster, Luca Antonia Grebe, Eva Forsten, Katharina Hürter, Jochen Büchs, Jørgen Magnus   +6 more
wiley   +1 more source

The WAC-downWAC domain in the yeast ISW2 nucleosome remodeling complex forms a structural module essential for ISW2 function but not cell viability. [PDF]

Epigenetics Chromatin
Singh AK, Grünert SI, Pfaller L, Mueller-Planitz F.   +3 more
europepmc   +1 more source

Online monitored characterization of Phocaeicola vulgatus for organic acid production using anaerobic microtiter plate cultivations

Biotechnology Progress, Volume 41, Issue 2, March/April 2025.
Abstract Phocaeicola vulgatus (formerly Bacteroides vulgatus), an anaerobic gut bacterium, produces several organic acids. Research on P. vulgatus is still in its infancy. However, a detailed understanding of P. vulgatus growth and metabolism is essential for its assessment as an organic acid producer.
Laura Keitel, Benjamin Schick, Gino Pohen, Stanislav Yordanov, Jochen Büchs   +4 more
wiley   +1 more source

Modeling Necroptotic and Pyroptotic Signaling in <i>Saccharomyces cerevisiae</i>. [PDF]

Biomolecules
Barbero-Úriz Ó, Valenti M, Molina M, Fernández-Acero T, Cid VJ.   +4 more
europepmc   +1 more source

Insights into the morphology‐productivity relationship of filamentous fungi through small‐scale cultivation and automated microscopy of Thermothelomyces thermophilus

Biotechnology Progress, EarlyView.
Abstract Filamentous fungi are a cornerstone in the biotechnological production of enzymes, proteins, and organic acids. However, challenges in understanding and controlling the relationship between morphology and productivity can limit their application.
Katja Rohr, Bertram Geinitz, Johannes Seiffarth, Aydin Anbarani, Sören Bernauer, Matthias Moch, Julia Tenhaef, Wolfgang Wiechert, Katharina Nöh, Marco Oldiges   +9 more
wiley   +1 more source

Long-read plasmid sequencing strategy for evaluating intrinsic instability of tandem gene arrays. [PDF]

MicroPubl Biol
Takesue H, Okada S, Ito T.
europepmc   +1 more source

A method for facile production of variable lymphocyte receptors using SHuffle Escherichia coli

Biotechnology Progress, EarlyView.
Abstract Variable lymphocyte receptors (VLRs) are the antigen receptors of jawless vertebrates such as lamprey. VLRs are of growing biotechnological interest for their ability to bind certain antigenic targets with higher affinity than traditional immunoglobulins.
Elizabeth A. Appelt, James B. Thoden, Candis Dancy, Hannah D. Bachmeier, Seth A. Gehrke, Melanie A. Staffenson, Ivan Rayment, Moriah E. Katt, Hazel M. Holden, Eric V. Shusta   +9 more
wiley   +1 more source

Effects of Yeast Products on the Apparent Total Tract Macronutrient Digestibility, Oxidative Stress Markers, Skin Measures, and Fecal Characteristics and Microbiota Populations of Healthy Adult Dogs. [PDF]

Animals (Basel)
De La Guardia Hidrogo VM, Rummell LM, Swanson KS.   +2 more
europepmc   +1 more source

Enhancing the performance of an in vitro RNA biosensor through iterative design of experiments

Biotechnology Progress, EarlyView.
Abstract The quality control of RNA has become increasingly crucial with the rise of mRNA‐based vaccines and therapeutics. However, conventional methods such as LC–MS often require specialized equipment and expertise, limiting their applicability to high throughput experiments.
Rochelle Aw, Karen Polizzi
wiley   +1 more source

Spontaneous Cell Detachment from Temperature Gradients: Getting the Method Ready for Antimicrobial Drug Testing at Cell Culture Level. [PDF]

Sensors (Basel)
Urbán CT, Bakhshi Sichani S, Ueda Modaffore G, Glorieux C, Gruber J, Yongabi D, Lettinga MP, Wagner P.   +7 more
europepmc   +1 more source