Results 21 to 30 of about 23,804 (206)

Adh1-Programmed SNF1 Phosphogradients Decrypt Morphogenesis in Candida albicans: Chemical Interrogation Unveils Hyphal Transition Thresholds. [PDF]

open access: yesMicrob Biotechnol
Based on the ADH1 knockout strain, HAQ was identified through forward/reverse genetic screening of 114 natural products and analysis of protein ligand and protein–protein interactions. With the help of HAQ, a new mechanism for regulating the phosphorylation level of Adh1–SNF1 has been revealed, in which HAQ binds to Adh1 to release dephosphorylation ...
Wang Z   +7 more
europepmc   +2 more sources

Binding of coenzymes to yeast alcohol dehydrogenase [PDF]

open access: yesJournal of the Serbian Chemical Society, 2010
In this work, the binding of coenzymes to yeast alcohol dehydrogenase (EC 1.1.1.1) were investigated. The main criterions were the change in the standard free energies for individual reaction steps, the internal equilibrium constants and the overall ...
VLADIMIR LESKOVAC   +4 more
doaj  

Immobilization and stabilization of alcohol dehydrogenase on polyvinyl alcohol fibre

open access: yesBiotechnology Reports, 2018
A polyvinyl alcohol (PVA) fibrous carrier has been chemically modified for the immobilization of yeast alcohol dehydrogenase (ADH) with an aim to increase its stability over a wide pH range, prolong its activity upon storage, and enhance its reusability.
Priydarshani Shinde   +4 more
doaj   +1 more source

Renewable synthesis of n-butyraldehyde from glucose by engineered Escherichia coli

open access: yesBiotechnology for Biofuels, 2017
Background n-Butyraldehyde is a high-production volume chemical produced exclusively from hydroformylation of propylene. It is a versatile chemical used in the synthesis of diverse C4–C8 alcohols, carboxylic acids, esters, and amines. Its high demand and
Jason T. Ku   +2 more
doaj   +1 more source

Cytosolic re-localization and optimization of valine synthesis and catabolism enables inseased isobutanol production with the yeast Saccharomyces cerevisiae

open access: yesBiotechnology for Biofuels, 2012
Background The branched chain alcohol isobutanol exhibits superior physicochemical properties as an alternative biofuel. The yeast Saccharomyces cerevisiae naturally produces low amounts of isobutanol as a by-product during fermentations, resulting from ...
Brat Dawid   +4 more
doaj   +1 more source

Logic‐Gated HSV‐TK/GCV Suicide Gene Circuit for Triple‐Negative Breast Cancer

open access: yesAdvanced Science, EarlyView.
The BRAS comprises two modular genetic components driven by distinct tumor‐specific promoters and a failsafe layer with the NOT gate. This multi‐input logic gate circuit enables precise, specific expression of HSV‐TK in breast cancer cells with hardly expression in normal cell and effectively inhibits tumor growth in a triple‐negative breast cancer ...
Shasha Tang   +10 more
wiley   +1 more source

Efficient chiral synthesis by Saccharomyces cerevisiae spore encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase in organic solvents

open access: yesMicrobial Cell Factories, 2019
Background Saccharomyces cerevisiae AN120 osw2∆ spores were used as a host with good resistance to unfavorable environment. This work was undertaken to develop a new yeast spore-encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II ...
Jingxin Rao   +5 more
doaj   +1 more source

Immunoproteomics reveal increased serum IgG3/5 binding to Dermatophagoides and yeast protein antigens in severe equine asthma in a preliminary study

open access: yesFrontiers in Immunology, 2023
IntroductionSevere equine asthma (SEA) is a common, chronic respiratory disease of horses characterized by hyperreactivity to hay dust which has many similarities to severe neutrophilic asthma in humans.
Christiane L. Schnabel   +11 more
doaj   +1 more source

Dye Affinity Labelling of Yeast Alcohol Dehydrogenase [PDF]

open access: yesJournal of Enzyme Inhibition, 2000
The interaction of yeast alcohol dehydrogenase (ADH) with the reactive chlorotriazine dye Vilmafix Blue A-R (VBAR) was studied. VBAR was purified to homogeneity on lipophilic Sephadex LH-20 and characterised by reverse phase HPLC and analytical TLC.
openaire   +2 more sources

Engineering New‐to‐Nature Biological Pathways for β,γ‐Alkanediol Synthesis

open access: yesAdvanced Science, EarlyView.
β,γ‐Alkanediols are value‐added chemicals to be used as functional solvents, biofuels, and cosmetic components. This work demonstrates a carboligation‐mediated approach for synthesis of linear chain β,γ‐alkanediols in Escherichia coli, namely, hexane‐2,3‐diol (2,3‐HDO) and pentane‐2,3‐diol (2,3‐PDO). The engineered E. coli cells produce 152.2 mm (17.98
Haofeng Chen   +6 more
wiley   +1 more source

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