Smc5/6 coordinates formation and resolution of joint molecules with chromosome morphology to ensure meiotic divisions [PDF]
, 2013 During meiosis, Structural Maintenance of Chromosome (SMC) complexes underpin two fundamental features of meiosis: homologous recombination and chromosome segregation.
Philip W. Jordan (265255) +61 more
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Heterologous overexpression and mutagenesis of the human bile salt export pump (ABCB11) using DREAM (Directed REcombination-Assisted Mutagenesis). [PDF]
PLoS ONE, 2011 Homologous recombination in Saccharomyces cerevisiae is a well-studied process. Here, we describe a yeast-recombination-based approach to construct and mutate plasmids containing the cDNA of the human bile salt export pump (BSEP) that has been shown to ...
Jan Stindt +7 more
doaj +1 more source
Rescue of end fragments of yeast artificial chromosomes by homologous recombination in yeast
Nucleic Acids Research, 1991 Yeast artificial chromosomes (YACs) provide a powerful tool for the isolation and mapping of large regions of mammalian chromosomes. We developed a rapid and efficient method for the isolation of DNA fragments representing the extreme ends of YAC clones by the insertion of a rescue plasmid into the YAC vector by homologous recombination.
G G, Hermanson +3 more
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How to halve ploidy : lessons from budding yeast meiosis [PDF]
, 2012 Maintenance of ploidy in sexually reproducing organisms requires a specialized form of cell division called meiosis that generates genetically diverse haploid gametes from diploid germ cells.
Kerr, Gary W. +3 more
core +1 more source
Anais da Academia Brasileira de Ciências, 2013 RNA replicon derived from Flavivirus genome is a valuable tool for studying viral replication independent of virion assembly and maturation, besides being a great potencial for heterologous gene expression.
Sabrina R.A. Queiroz +5 more
doaj +1 more source
Recovery of arrested replication forks by homologous recombination is error-prone. [PDF]
PLoS Genetics, 2012 Homologous recombination is a universal mechanism that allows repair of DNA and provides support for DNA replication. Homologous recombination is therefore a major pathway that suppresses non-homology-mediated genome instability.
Ismail Iraqui +6 more
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HOMOLOGOUS RECOMBINATION BETWEEN EPISOMAL PLASMIDS AND CHROMOSOMES IN YEAST
Genetics, 1983 ABSTRACT We have observed genetic recombination between ura3 - mutations (among them extensive deletions) carried on "episomal" (i.e., 2μ DNA-containing) plasmids and other ura3 - alleles present at the normal chromosomal URA3 locus.
S C, Falco, M, Rose, D, Botstein
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Oligonucleotide-Mediated, PCR-Independent Cloning by Homologous Recombination
BioTechniques, 2001 We have developed an oligonucleotide-mediated cloning technique based on homologous recombination in Saccharomyces cerevisiae that allows precise DNA sequences to be transferred independent of restriction enzymes and PCR.
D.J. DeMarini +6 more
doaj +1 more source
Development of a targeted flip-in system in avian DT40 cells. [PDF]
PLoS ONE, 2015 Gene-targeting to create null mutants or designed-point mutants is a powerful tool for the molecular dissection of complex phenotypes involving DNA repair, signal transduction, and metabolism.
Kaori Kobayashi +4 more
doaj +1 more source
Ultrafine anaphase bridges, broken DNA and illegitimate recombination induced by a replication fork barrier [PDF]
, 2011 Most DNA double-strand breaks (DSBs) in S- and G2-phase cells are repaired accurately by Rad51-dependent sister chromatid recombination. However, a minority give rise to gross chromosome rearrangements (GCRs), which can result in disease/death.
Ledesma, Jennifer +21 more
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