Results 41 to 50 of about 613,719 (256)

TRF2-RAP1 is required to protect telomeres from engaging in homologous recombination-mediated deletions and fusions

open access: yesNature Communications, 2016
While yeast Rap1 regulates telomere length and protects telomeres from non-homologous end joining, its role in higher eukaryotes is controversial.
Rekha Rai   +3 more
doaj   +1 more source

Ultra-low background DNA cloning system. [PDF]

open access: yesPLoS ONE, 2013
Yeast-based in vivo cloning is useful for cloning DNA fragments into plasmid vectors and is based on the ability of yeast to recombine the DNA fragments by homologous recombination. Although this method is efficient, it produces some by-products. We have
Kenta Goto, Yukio Nagano
doaj   +1 more source

Recombination machinery engineering for precise genome editing in methylotrophic yeast Ogataea polymorpha

open access: yesiScience, 2021
Summary: Methanol biotransformation can expand biorefinery substrate spectrum other than biomass by using methylotrophic microbes. Ogataea (Hansenula) polymorpha, a representative methylotrophic yeast, attracts much attention due to its thermotolerance ...
Jiaoqi Gao   +3 more
doaj   +1 more source

Temporal characterization of homology-independent centromere coupling in meiotic prophase.

open access: yesPLoS ONE, 2010
BackgroundOver the past thirty years several reports of the pairing or association of non-homologous centromeres during meiotic prophase have appeared in the literature.
David Obeso, Dean S Dawson
doaj   +1 more source

Site-Directed Mutagenesis and Generation of Chimeric Viruses by Homologous Recombination in Yeast to Facilitate Analysis of Plant-Virus Interactions

open access: yesMolecular Plant-Microbe Interactions, 2004
A yeast homologous recombination system was used to generate mutants and chimeras in the genome of Potato leafroll virus (PLRV). A yeast-bacteria shuttle vector was developed that allows mutants and chimeras generated in yeast to be transformed into ...
Delin Liang   +3 more
doaj   +1 more source

UGDR: a generic pipeline to detect recombined regions in polyploid and complex hybrid yeast genomes

open access: yesBMC Bioinformatics, 2022
Motivation In eukaryotes, homologous recombination between the parental genomes frequently occurs during the evolutionary conserved process of meiosis, generating the genetic diversity transmitted by the gametes.
Amina Bedrat
doaj   +1 more source

ruvA Mutants that resolve Holliday junctions but do not reverse replication forks [PDF]

open access: yes, 2008
RuvAB and RuvABC complexes catalyze branch migration and resolution of Holliday junctions (HJs) respectively. In addition to their action in the last steps of homologous recombination, they process HJs made by replication fork reversal, a reaction which ...
Bradley Alison Sylvia   +14 more
core   +2 more sources

Characterization of a novel human SMC heterodimer homologous to the Schizosaccharomyces pombe Rad18/Spr18 complex [PDF]

open access: yes, 2001
The structural maintenance of chromosomes (SMC) protein encoded by the fission yeast rad18 gene is involved in several DNA repair processes and has an essential function in DNA replication and mitotic control.
Moghraby, Jeelan S.   +5 more
core   +4 more sources

Slx4 scaffolding in homologous recombination and checkpoint control: lessons from yeast [PDF]

open access: yesChromosoma, 2016
Homologous recombination-mediated DNA repair is essential for maintaining genome integrity. It is a multi-step process that involves resection of DNA ends, strand invasion, DNA synthesis and/or DNA end ligation, and finally, the processing of recombination intermediates such as Holliday junctions or other joint molecules. Over the last 15 years, it has
J. R. Cussiol   +3 more
openaire   +2 more sources

Characterisation of a Holliday junction-resolving enzyme from Schizosaccharomyces pombe [PDF]

open access: yes, 2014
We thank the Cancer Research Campaign for financial support.The rearrangement and repair of DNA by homologous recombination involves the creation of Holliday junctions, which are cleaved by a class of junction-specific endonucleases to generate ...
Lilley, DMJ, White, Malcolm Frederick
core   +2 more sources

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