Results 11 to 20 of about 613,719 (256)
Cell Biology of Homologous Recombination in Yeast [PDF]
, 2011 Homologous recombination is an important pathway for error-free repair of DNA lesions, such as single- and double-strand breaks, and for rescue of collapsed replication forks. Here, we describe protocols for live cell imaging of single-lesion recombination events in the yeast Saccharomyces cerevisiae using fluorescence microscopy.
Eckert-Boulet, Nadine Valerie +2 more
openaire +4 more sources
Use of a unique reporter cassette to examine 5´ to 3´ resection at a site specific DNA double-strand break during meiosis [PDF]
, 2006 Meiotic recombination in Saccharomyces cerevisiae is initiated by the formation of DNA double strand breaks (DSBs), which are created by Spoil protein.
Bishop-Bailey, Anna
core +6 more sources
Telomerase Repairs Collapsed Replication Forks at Telomeres
Cell Reports, 2020 Summary: Telomeres are difficult-to-replicate sites whereby replication itself may threaten telomere integrity. We investigate, in fission yeast, telomere replication dynamics in telomerase-negative cells to unmask problems associated with telomere ...
Samah Matmati +3 more
doaj +1 more source
Rapid and Efficient Plasmid Construction by Homologous Recombination in Yeast [PDF]
Cold Spring Harbor Protocols, 2015 The cloning of DNA fragments is a fundamental aspect of molecular biology. Traditional DNA cloning techniques rely on the ligation of an insert and a linearized plasmid that have been digested with restriction enzymes and the subsequent introduction of the ligated DNA into Escherichia coli for propagation.
Jolanda, van Leeuwen +3 more
openaire +2 more sources
eLife, 2023 Arrested replication forks, when restarted by homologous recombination, result in error-prone DNA syntheses and non-allelic homologous recombination. Fission yeast RTS1 is a model fork barrier used to probe mechanisms of recombination-dependent restart ...
Alice M Budden +6 more
doaj +1 more source
Biochemical properties of fission yeast homologous recombination enzymes
Current Opinion in Genetics & Development, 2021 Homologous recombination (HR) is a universal phenomenon conserved from viruses to humans. The mechanisms of HR are essentially the same in humans and simple unicellular eukaryotes like yeast. Two highly diverged yeast species, Saccharomyces cerevisiae and Schizosaccharomyces pombe, have proven exceptionally useful in understanding the fundamental ...
Hideo Tsubouchi +2 more
openaire +2 more sources
Microorganisms, 2021 Metschnikowia pulcherrima is a non-conventional yeast with the potential to be used in biotechnological processes, especially involving low-cost feedstock exploitation.
Mauro Moreno-Beltrán +3 more
doaj +1 more source
Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil [PDF]
Memorias do Instituto Oswaldo Cruz, 2015 Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions.
Jefferson José da Silva Santos +5 more
doaj +3 more sources
Biomolecules, 2022 Meiotic recombination is a pivotal event that ensures faithful chromosome segregation and creates genetic diversity in gametes. Meiotic recombination is initiated by programmed double-strand breaks (DSBs), which are catalyzed by the conserved Spo11 ...
Kouji Hirota
doaj +1 more source
Mutational Effects of Mobile Introns on the Mitochondrial Genomes of Metschnikowia Yeasts
Frontiers in Genetics, 2021 It has been argued that DNA repair by homologous recombination in the context of endonuclease-mediated cleavage can cause mutations. To better understand this phenomenon, we examined homologous recombination following endonuclease cleavage in a native ...
Scout R. L. Thompson +3 more
doaj +1 more source