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Homologous Recombination: A GRAS Yeast Genome Editing Tool [PDF]
Fermentation, 2020 The fermentation industry is known to be very conservative, relying on traditional yeast management. Yet, in the modern fast-paced world, change comes about in facets such as climate change altering the quality and quantity of harvests, changes due to ...
Beatrice Bernardi, Jürgen Wendland
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Optimizing Yeast Homologous Recombination for Splicing Large Coronavirus Genome Fragments [PDF]
International Journal of Molecular SciencesReverse genetics is a useful tool for studying viruses and developing vaccines for coronaviruses. However, constructing and manipulating the coronavirus genome in Escherichia coli can be time-consuming and challenging due to its large size and instability.
Gang Liu
exaly +4 more sources
Regulation of homologous recombination at telomeres in budding yeast [PDF]
FEBS Letters, 2010 Homologous recombination is suppressed at normal length telomere sequences. In contrast, telomere recombination is allowed when telomeres erode in the absence of telomerase activity or as a consequence of nucleolytic degradation or incomplete replication.
Michael Lisby, Nadine Eckert-Boulet
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Creating Precise GFP Fusions in Plasmids Using Yeast Homologous Recombination [PDF]
BioTechniques, 2003 Using a combination of primer amplification, homologous recombination, and yeast genetics, we established a method for creating precise promoter and protein fusions in genes originating from organisms other than yeast.
Nancy C. Hawkins +2 more
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PLoS Genetics, 2016 The Saccharomyces cerevisae RAD3 gene is the homolog of human XPD, an essential gene encoding a DNA helicase of the TFIIH complex involved in both nucleotide excision repair (NER) and transcription.
Sabrina L Andersen +6 more
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Plasmid Construction by Linker-Assisted Homologous Recombination in Yeast [PDF]
BioTechniques, 2001 Paul L. Gunyuzlu +2 more
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Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.
PLoS Genetics, 2014 Fbh1, an F-box helicase related to bacterial UvrD, has been proposed to modulate homologous recombination in fission yeast. We provide several lines of evidence for such modulation.
Yasuhiro Tsutsui +6 more
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PLoS Genetics, 2023 Although homologous recombination between transposable elements can drive genomic evolution in yeast by facilitating chromosomal rearrangements, the details of the underlying mechanisms are not fully clarified.
Lei Qi +9 more
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The Shu complex prevents mutagenesis and cytotoxicity of single-strand specific alkylation lesions
eLife, 2021 Three-methyl cytosine (3meC) are toxic DNA lesions, blocking base pairing. Bacteria and humans express members of the AlkB enzymes family, which directly remove 3meC. However, other organisms, including budding yeast, lack this class of enzymes.
Braulio Bonilla +16 more
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To nick or not to nick: comparison of I-SceI single- and double-strand break-induced recombination in yeast and human cells. [PDF]
PLoS ONE, 2014 Genetic modification of a chromosomal locus to replace an existing dysfunctional allele with a corrected sequence can be accomplished through targeted gene correction using the cell's homologous recombination (HR) machinery.
Samantha S Katz +2 more
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