Results 21 to 30 of about 107,862 (214)

Toxicological assessment of trace β-diketone antibiotic mixtures on zebrafish (Danio rerio) by proteomic analysis. [PDF]

PLoS ONE, 2014
β-Diketone antibiotics (DKAs) can produce chronic toxicity in aquatic ecosystems due to their pseudo-persistent in the environment. In this study, after long-term DKA exposure to zebrafish (Danio rerio), 47 protein spots had greater than 2-fold ...
Xiaohan Yin, Huili Wang, Yuna Zhang, Randy A Dahlgren, Hongqin Zhang, Mengru Shi, Ming Gao, Xuedong Wang   +7 more
doaj   +1 more source

Neuronal cell culture from transgenic zebrafish models of neurodegenerative disease

Biology Open, 2018
We describe a protocol for culturing neurons from transgenic zebrafish embryos to investigate the subcellular distribution and protein aggregation status of neurodegenerative disease-causing proteins.
Jamie R. Acosta, Maxinne Watchon, Kristy C. Yuan, Jennifer A. Fifita, Adam J. Svahn, Emily K. Don, Claire G. Winnick, Ian P. Blair, Garth A. Nicholson, Nicholas J. Cole, Claire Goldsbury, Angela S. Laird   +11 more
doaj   +1 more source

Zebavidin - An Avidin-Like Protein from Zebrafish

PLoS ONE, 2013
The avidin protein family members are well known for their high affinity towards D-biotin and high structural stability. These properties make avidins valuable tools for a wide range of biotechnology applications. We have identified a new member of the avidin family in the zebrafish (Danio rerio) genome, hereafter called zebavidin.
Taskinen, Barbara, Zmurko, Joanna, Ojanen, Markus, Kukkurainen, Sampo, Parthiban, Marimuthu, Määttä, Juha, Leppiniemi, Jenni, Jänis, Janne, Parikka, Mataleena, Turpeinen, Hannu, Rämet, Mika, Pesu, Marko, Johnson, Mark, Kulomaa, Markku S, Airenne, Tomi, Hytönen, Vesa P   +15 more
openaire   +6 more sources

Extraction Protocols for Individual Zebrafish's Ventricle Myosin and Skeletal Muscle Actin for In vitro Motility Assays

Frontiers in Physiology, 2017
The in vitro motility assay (IVMA) is a technique that enables the measurement of the interaction between actin and myosin providing a relatively simple model to understand the mechanical muscle function. For actin-myosin IVMA, myosin is immobilized in a
Lisa-Mareike Scheid, Cornelia Weber, Nasrin Bopp, Matias Mosqueira, Rainer H. A. Fink   +4 more
doaj   +1 more source

Proximity Dependent Biotin Labelling in Zebrafish for Proteome and Interactome Profiling

Bio-Protocol, 2021
Identification of protein interaction networks is key for understanding intricate biological processes, but mapping such networks is challenging with conventional biochemical methods, especially for weak or transient interactions.
Zherui Xiong, Harriet Lo, Kerrie- McMahon, Robert Parton, Thomas Hall   +4 more
doaj   +1 more source

Macrophage‐stimulating protein and calcium homeostasis in zebrafish

The FASEB Journal, 2012
To systematically identify novel gene functions essential for osteogenesis and skeletal mineralization, we performed a forward genetic mutagenesis screen in zebrafish and isolated a mutant that showed delayed skeletal mineralization. Analysis of the mutant phenotype in an osterix :nuclear‐GFP
Huitema, L.F.A., Renn, J., Logister, I., Gray, J.K., Waltz, S.E., Flik, G., Schulte-Merker, S.   +6 more
openaire   +4 more sources

Retinal degeneration in rpgra mutant zebrafish

Frontiers in Cell and Developmental Biology, 2023
Introduction: Pathogenic mutations in RPGRORF15, one of two major human RPGR isoforms, were responsible for most X-linked retinitis pigmentosa cases. Previous studies have shown that RPGR plays a critical role in ciliary protein transport.
Xiliang Liu, Xiliang Liu, Shanshan Han, Shanshan Han, Fei Liu, Fei Liu, Shanshan Yu, Shanshan Yu, Yayun Qin, Yayun Qin, Jingzhen Li, Danna Jia, Pan Gao, Xiang Chen, Zhaohui Tang, Mugen Liu, Yuwen Huang   +16 more
doaj   +1 more source

Characterization of the RNA-binding protein Musashi1 in zebrafish

Brain Research, 2012
Musashi (Msi) is an evolutionarily conserved gene family of RNA-binding proteins (RBPs) that is preferentially expressed in the nervous system. The first member of the Msi family was identified in Drosophila. Drosophila Msi plays an important role in regulating asymmetric cell division of the sensory organ precursor cells.
Shibata, Shinsuke, Umei, Masahiko, Kawahara, Hironori, Yano, Masato, Makino, Shinji, Okano, Hideyuki   +5 more
openaire   +2 more sources

Crk adaptor proteins are necessary for the development of the zebrafish retina

Developmental Dynamics, 2021
AbstractBackgroundThe development of the central nervous system (CNS) requires critical cell signaling molecules to coordinate cell proliferation and migration in order to structure the adult tissue. Chicken tumor virus #10 Regulator of Kinase (CRK) and CRK‐like (CRKL) are adaptor proteins with pre‐metazoan ancestry and are known to be required for ...
Helaina R. Stergas, Zoë Kalbag, Riley M. St. Clair, Jared C. Talbot, Bryan A. Ballif, Alicia M. Ebert   +5 more
openaire   +3 more sources

Profiling subcellular localization of nuclear-encoded mitochondrial gene products in zebrafish

Life Science Alliance, 2023
The vast majority (99%) of mitochondrial proteins are encoded by the nuclear genome, synthesized in the cytosol and imported into the organelle. Here we study the principles of mitochondrial import in vivo from the RNA perspective using zebrafish.
Barbara Uszczynska-Ratajczak, Sreedevi Sugunan, Monika Kwiatkowska, Maciej Migdal, Silvia Carbonell-Sala, Anna Sokol, Cecilia L Winata, Agnieszka Chacinska   +7 more
doaj   +1 more source