Results 61 to 70 of about 804,350 (314)

SuFEx‐Enabled Reprogramming of Flavonoids for Selective α‐Glucosidase Covalent Inhibition

Advanced Science, EarlyView.
Selective inhibition of intestinal α‐glucosidase remains limited by poor enzyme specificity and off‐target metabolic effects. Here, SuFEx click chemistry is used to reprogram natural flavonoids into covalent inhibitors with enhanced α‐glucosidase selectivity and controlled reactivity. This strategy enables effective regulation of carbohydrate digestion
Fengyu Guo, Liwei Zhang, Minlong Wang, Yanjin Du, Tianyu Zhang, Hao Chen, Jiazeng Sun, Yang Yu, Zude He, Jie An, Xiaoxu Zhang, Weilin Lin, Fazheng Ren, Pengjie Wang, Ping Liu   +14 more
wiley   +1 more source

Preparation and properties of α-amylase immobilized on silk fibroin

浙江大学学报. 农业与生命科学版, 2002
Cavernous basified fibroin was prepared from degumed silk which pretreated with dilute alkali solution and served as supporter for immobilization of α-amylase to form the basified fibroin immobilized α-amylase, BFIA.
ZHU Xiang-rui, XU Jun-liang
doaj   +1 more source

Studies on a-amylase inhibitors from seeds of sorghum bicolor [PDF]

, 1991
Six inhibitors (Slal, SIa2, SIa3, SIa4, SIa5 and SIa6) of a-amylase from mammalian, insect, bacterial and fungal sources were purified from seeds of Sorghum bicolor (L) Moench by saline extraction, precipitation with ammonium sulphate, affinity ...
Junior, Carlos Bloch
core  

Discriminator‐Guided Inverse Folding for Multi‐Property Protein Design

Advanced Science, EarlyView.
Discriminator‐Guided Inverse Folding (DGIF) integrates multiple property predictors trained from single‐property datasets to guide protein sequence generation from a backbone structure. DGIF enables simultaneous improvement of thermostability and solubility without requiring multi‐property annotated datasets and generates designs that move toward the ...
Yuchuan Zheng, Chuyi Liu, Zhaoming Liu, Mao Su, Chenyu Tang, Xiang Zheng, Hao Zhang, Jingyuan Li   +7 more
wiley   +1 more source

Bacillus subtilisα-amylase: the rate limiting step of secretion is growth phase-independent [PDF]

FEMS Microbiology Letters, 1999
When Bacillus subtilis alpha-amylase was expressed under the control of sacR in a degU32(Hy) strain, the production of exoenzyme occurred during both the exponential and stationary phases of growth. In each phase, pulse-chase experiments showed that the rate-limiting step of the secretion process was the release of the processed form of the protein in ...
E, Haddaoui, R, Chambert, M F, Petit-Glatron, O, Lindy, M, Sarvas   +4 more
openaire   +2 more sources

Starch‐Molded Snacks Enriched With Nance (Byrsonima crassifolia L.) Fruit Flour Functional Potential

AgriFood: Journal of Agricultural Products for Food, EarlyView.
Nance flour enriches starch‐molded snacks with antioxidants and fiber. These innovative snacks offer high phenolic bioaccessibility and improved sensory appeal, valorizing an underutilized fruit for sustainable food development. ABSTRACT This study aimed to develop starch‐molded snacks enriched with nance (Byrsonima crassifolia L.) flour, a native ...
Mariana Carolina Fregoso‐Ultreras, Cinthia Jazmin Villegas‐Ornelas, Victor Manuel Zamora‐Gasga, Jorge Alberto Sánchez‐Burgos, Sonia Guadalupe Sáyago‐Ayerdi, Francisco Javier Blancas‐Benitez   +5 more
wiley   +1 more source

AS‐pHopt: An Optimal pH Prediction Model Enhanced by Active Site of Enzymes

Advanced Intelligent Discovery, EarlyView.
To address the low accuracy of enzyme optimal pH (pHopt) prediction, this study develops active site‐based pHopt (AS‐pHopt), a prediction model enhanced by active site information and pseudo‐label prediction. Integrating key structural and physicochemical features affecting enzyme pHopt, AS‐pHopt uses Evolutionary Scale Modeling (ESM)‐2 with active ...
Wenxiang Song, Xinmin Li, Hongbo Yu, Le Xiong, Guixia Liu, Weihua Li, Yun Tang   +6 more
wiley   +1 more source

Cloning strategies for the isolation of an a-amylase inhibitor/endochitinase gene from the seeds of Coix lachryma-jobi [PDF]

, 1993
The aim of this work was to isolate the gene encoding a bifunctional a-amylase inhibitor/endochitinase protein from the seeds of Coix lachryma-jobi, a tropical cereal.
Fairweather, Donna, Fairweather, D
core  

Genomic and functional characterization of coleopteran insect-specific α-amylase inhibitor gene from Amaranthus species

, 2017
The smallest 32 amino acid α-amylase inhibitor from Amaranthus hypochondriacus (AAI) is reported. The complete gene of pre-protein (AhAI) encoding a 26 amino acid (aa) signal peptide followed by the 43 aa region and the previously identified 32 aa ...
Amey J. Bhide, Ashok P. Giri, Vidya S. Gupta, Gupta, Vidya S., V. L. Maheshwari, Giri, Ashok P., Kabita Bhattacharjee, Channale, Sonal M., Yashpal Yadav, Pankaj K. Pawar, Pawar, Pankaj K., Ramasamy, Sureshkumar, Bhide, A., Maheshwari, V. L., Sureshkumar Ramasamy, Yadav, Yashpal, Sonal M. Channale, Bhattacharjee, Kabita   +17 more
core   +1 more source

Starch-iodine assay method underestimates α-amylase inhibitory potential of antioxidative compounds and extracts

, 2020
Starch-iodine assay method for the determination of α-amylase activity is also used in screening extracts for α-amylase inhibitors. However, there are indications that this method may not be appropriate for screening some classes of compounds or plant ...
Ezeigwe, O.C., Obiajulu Ezeigwe, Ononamadu, C.J., Owolarafe, T.A., Tajudeen Lawal, Umeoguaju, U.F., Godwin Ihegboro, Ihegboro, G.O., Lawal, T.A., Ibrahim Aminu, Aminu, I., Tajudeen Owolarafe, Uchenna Umeoguaju, Salawu, K., Chimaobi Ononamadu, Kailani Salawu   +15 more
core   +1 more source