Results 91 to 100 of about 143,975 (276)

MOESM1 of Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris

open access: yes, 2016
Additional file 1. Stoichiometric and carbon atom transition model for P. pastoris.
Liu, Ping   +7 more
openaire   +1 more source

The CBRB regulon: Promoter dissection reveals novel insights into the CbrAB expression network in Pseudomonas putida [PDF]

open access: yes, 2018
CbrAB is a high ranked global regulatory system exclusive of the Pseudomonads that responds to carbon limiting conditions. It has become necessary to define the particular regulon of CbrB and discriminate it from the downstream cascades through other ...
Andújar, Eloísa   +6 more
core   +3 more sources

Intrinsically Disordered Protein‐Inspired Nanovector‐Based Coacervates for the Direct Cytosolic Transport of Biomacromolecules

open access: yesAdvanced Materials, EarlyView.
Intrinsically disordered protein‐inspired nanovectors (IDP‐NVs) form stable nanocoacervates (NCs) with diverse biomacromolecules. In situ conformational changes confer stability and adaptability to NCs under dynamically changing physiological conditions.
Soyeong Jin   +14 more
wiley   +1 more source

Phytochemical analysis, antioxidant and in vitro β-galactosidase inhibition activities of Juniperus phoenicea and Calicotome villosa methanolic extracts

open access: yesBMC Chemistry, 2021
Background Juniperus Phoenicea (JP) and Calicotome Villosa (CV) are used by Jordanian populations as herbal remedies in traditional medicine. Herein, the phytochemical contents of their methanolic extracts were analyzed and their antioxidant as well as ...
Ahmed Al-Mustafa   +3 more
doaj   +1 more source

MOESM4 of Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris

open access: yes, 2016
Additional file 4. Trends of the MID of intracellular free amino acids during 13C labeling experiments on Glc and on Glc/Glu.
Liu, Ping   +7 more
openaire   +1 more source

A rapid permeabilization procedure for accurate quantitative determination of β-galactosidase activity in yeast cells [PDF]

open access: yesFEMS Microbiology Letters, 1995
A procedure is described which allows the rapid permeabilization of yeast cells, Schizosaccharomyces pombe and Saccharomyces cerevisiae, for quantitative in situ assays of beta-galactosidase activity. Yeast cells are permeabilized by incubation in buffer containing 0.2% of the detergent sodium lauroyl sarcosinate without any need for washing or ...
openaire   +2 more sources

Effect of moderated pressure on the activity and termostability of three microbial enzymes : catalase, β-galactosidase and alcohol dehydrogenase [PDF]

open access: yes, 2005
The effect of moderate gas pressure on the activity and termostability of three microbial enzymes: catalase from Aspergillus niger, β-galactosidase from Escherichia coli and alcohol dehydrogenase from Saccharomyces cerevisiae was study.
Belo, Isabel, Mota, M., Ribeiro, Helena
core  

ROS Activated NETosis of Bone Marrow CD55+ Intermediate Mature Neutrophils Through HIF1α‐PADI4 Pathway to Initiate Bone Aging

open access: yesAdvanced Science, EarlyView.
In this study, we find CD55+ neutrophils show activated NETosis within bone marrow, induce BMSC senescence and osteogenesis inhibition, finally leading to bone aging initiation. Mechanistically, ROS synergizes with the CD55‐driven HIF1α‐PADI4 pathway to promote NETosis.
Yutong Guo   +6 more
wiley   +1 more source

Mettl3‐Mediated m6A Modification Represents a Novel Therapeutic Target for FSGS

open access: yesAdvanced Science, EarlyView.
This study explores the roles of Mettl3‐induced N6‐methyladenosine (m6A) modifications in Focal segmental glomerulosclerosis (FSGS). The findings reveal that inhibition of Mettl3 results in podocyte injury by modulating the TJP1CDC42 pathway. Moreover, Administration of N6‐methyladenosine attenuates the FSGS phenotype in WT mice induced by Adriamycin ...
Fubin Zhu   +14 more
wiley   +1 more source

Induction of fibroblast senescence generates a non-fibrogenic myofibroblast phenotype that differentially impacts on cancer prognosis [PDF]

open access: yes, 2016
Cancer-associated fibroblasts (CAF) remain a poorly characterized, heterogeneous cell population. Here we characterized two previously described tumor-promoting CAF sub-types, smooth muscle actin (SMA)-positive myofibroblasts and senescent fibroblasts ...
Brennan, PA   +26 more
core   +2 more sources

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