Results 91 to 100 of about 807,747 (247)

Expression and nucleotide sequence of the Clostridium acetobutylicum β-galactosidase gene cloned in Escherichia coli

, 1991
A gene library for Clostridium acetobutylicum NCIB 2951 was constructed in the broad-host-range cosmid pLAFR1, and cosmids containing the β-galactosidase gene were isolated by direct selection for enzyme activity on X-Gal (5-bromo-4-chloro-3-indoly-β-D ...
Maddox, Ian S., Pearce, Lindsay, Hancock Kerrie R., Rockman, Eva, Young, Carolyn A., Scott, Barry   +5 more
core   +1 more source

FUCA2 Sustains AKT Signaling and Suppresses Senescence by Antagonizing FUT3‐Mediated ErbB3 Fucosylation in Lung Adenocarcinoma

Advanced Science, EarlyView.
ABSTRACT While targeted therapies have improved outcomes in lung adenocarcinoma (LUAD), many patients still lack targetable mutations. Here, we identified alpha‐L‐fucosidase 2 (FUCA2) as a crucial driver of LUAD by preventing cellular senescence. Mechanistically, through the restriction of fucosyltransferase 3 (FUT3)‐mediated α‐1,3‐fucosylation of ...
Lu Chen, Jintao Guo, Fei Li, Mingjie Gao, Runyang Li, Zhaozhang Huang, Yaolin Zheng, Chunyi Gao, Jihuan Hou, Qiang Yu, Bowen Zheng, Xuemei Chen, Wenqing Zhang, Xiaoting Hong, Yali Zheng, Daxuan Wang, Qiyuan Li, Tianhui Hu, Yan‐yan Zhan   +18 more
wiley   +1 more source

Deproteinization: an integrated-solution approach to increase efficiency in β-galactosidase production using cheese whey powder (CWP) solution

Revista Ambiente & Água, 2017
Whey is the liquid that results from the coagulation of milk during cheese manufacture. Cheese whey is also an important environmental pollution source.
Leandro Freire dos Santos, Cibely Maria Gonçalves, Priscila Lumi Ishii, Hélio Hiroshi Suguimoto   +3 more
doaj   +1 more source

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

, 2018
The Escherichia coli LacZ gene, encoding β-galactosidase, is largely used as a reporter for gene expression and as a tracer in cell lineage studies. The classical histochemical reaction is based on the hydrolysis of the substrate X-gal in combination ...
Rodríguez Martín, Iván, Muñoz Sáez, Emma, Blanco, María José, Blanco Fernández de Valderrama, María José, Sánchez-Camacho Blázquez, Cristina, Rodríguez-Martín, Iván, Marchena, Miguel Ángel, Rodríguez Learte, Ana Isabel, Marchena, Miguel, Muñoz-Sáez, Emma, Cid, María Antonia, Cid Torres, María Antonia, Learte, Ana   +12 more
core   +1 more source

Bioadhesive Hydrogel With Polyphenol‐Armored Nanogene Rejuvenates Chondrocyte Senescence for Aged Osteoarthritis Therapy

Advanced Science, EarlyView.
An adhesive hydrogel depot with polyphenol‐armored nanogenes is developed for treating aged osteoarthritis. This system maintains miR‐140 bioactivity, prolongs its intra‐articular retention, and enhances transfection into chondrocytes, leading to the downregulation of senescence‐associated pathways.
Liwei Yan, Runze Yang, Ting Zhou, Tianhao Xu, Yongqi Li, Yuelin Hu, Minghao Ge, Lei Zhang, Xiong Lu, Chaoming Xie, Weili Fu   +10 more
wiley   +1 more source

Purification and some properties of phospho-β-galactosidase from the Gram-negative oral bacterium Leptotrichia buccalis ATCC 14201 [PDF]

FEMS Microbiology Letters, 2002
Phospho-beta-galactosidase (P-beta-gal; EC 3.2.1.85) is induced during growth of Leptotrichia buccalis ATCC 14201 on lactose and lactulose. The enzyme has been purified to electrophoretic homogeneity (M(r) approximately 53 kDa, pI approximately 4.8), and kinetic parameters have been determined using the chromogenic analog o-nitrophenyl-beta-D ...
openaire   +2 more sources

Light‐Switched Mesenchymal Stem Cells for In Situ Exosome Amplification in Craniofacial Bone Defect Reconstruction

Advanced Science, EarlyView.
Light‐switchable MSCs (MSC‐UCNPs) were constructed by intracellular incorporation of UCNPs. Upon 980 nm irradiation, UCNPs emitted localized ultraviolet light (365 nm), activating the ROS/HEXB/LAMP1 signaling pathway to suppress lysosome–multivesicular body fusion and thereby enhance exosome biogenesis. Embedded within an injectable hydrogel, MSC‐UCNPs
Tingting Wu, Yajing Liu, Shuman Wang, Xiaoming Bai, Luyun Zhang, Yuwei Liu, Zhiwen Fu, Chen Shi   +7 more
wiley   +1 more source

Targeting Golgi–STING Signaling to Reprogram Innate and Adaptive Immunity for the Treatment of Implant‐Associated Infections

Advanced Science, EarlyView.
This study presents an ultrasound‐responsive nanoplatform, CS‐BT@MZ@NEs, with a BaTiO3/Mn‐Zif‐8 core and a chondroitin sulfate coating for Golgi targeting. By leveraging neutrophil hitchhiking, it enables targeted delivery to infection sites. Under ultrasound stimulation, CS‐BT@MZ@NEs generates ROS and modulates Golgi pH to activate cGAS–STING ...
Shicheng Huo, Naifeng Zhu, Zhuocheng Lyu, Yifei Liu, Zhenjiang Xu, Chun Zhou, Yaochao Zhao, Changgui Shi   +7 more
wiley   +1 more source

In vitro digestibility of oligosaccharides synthesized by dairy propionibacteria β-galactosidase from lactose, lactulose and lactitol

, 2023
Prebiotics synthesized by probiotic genera are of interest due to their specific selectivity. However, modifications of their composition and concentrations by digestion may affect their properties and effects in the host.
Fara, Agustina, Zárate, Gabriela, Hernández-Hernández, Oswaldo, Montilla, Antonia   +3 more
core   +1 more source

Oligopeptides/DNA Coacervate Droplets as Macromolecular Delivery Microcarriers

Advanced Science, EarlyView.
Oligopeptide–DNA coacervates formed via liquid–liquid phase separation serve as programmable carriers for macromolecular cargos, entering cells through lipid raft–associated pathways and subsequently undergoing enzyme triggered intracellular disassembly driven by DNA degradation processes, thereby enabling efficient, controlled, and spatially defined ...
Linyi Zhang, Chong Wang, Mengqi Han, Yi Yang, Teng Ma, Luoran Shang   +5 more
wiley   +1 more source