Results 81 to 90 of about 874,911 (274)

Predicting Enzyme Turnover Numbers and Enabling Rational Enzyme Evolution

Advanced Science, EarlyView.
MCKcat, a deep learning framework combining multi‐scale convolution and cross‐attention, accurately predicts enzyme turnover number (kcat) by fusing sequence and reaction representations. It couples with rational design in a two‐step strategy for Bacillus aryabhattai laccase engineering, achieving a 75% positive rate with synergistically enhanced ...
Fengya Ge, Xiangyang Ma, Jiyan Li, Mengxiang Wang, Fuping Lu, Yihan Liu, Lin Wang   +6 more
wiley   +1 more source

Mechanism of imidazole inhibition of a GH1 β‐glucosidase

FEBS Open Bio, 2023
Imidazole is largely employed in recombinant protein purification, including GH1 β‐glucosidases, but its effect on the enzyme activity is rarely taken into consideration.
Rafael S. Chagas, Felipe A. M. Otsuka, Mario A. R. Pineda, Roberto K. Salinas, Sandro R. Marana   +4 more
doaj   +1 more source

MOESM1 of The structural and functional contributions of β-glucosidase-producing microbial communities to cellulose degradation in composting

, 2018
Additional file 1: Figure S1. Differences in the abundance and expression of GH1B and GH3E genes in the natural compost and the inoculated compost.
Zang, Xiangyun, Liu, Meiting, Fan, Yihong, Xu, Jie, Xu, Xiuhong, Li, Hongtao   +5 more
openaire   +1 more source

Integration of the D‐Genome Reshapes Gene Transcription, Chromatin Architecture, and Metabolome of Allohexaploid Wheat, Leading to Enhanced Environmental Adaptability

Advanced Science, EarlyView.
Integration of the D‐genome reshapes gene transcription, chromatin architecture, and the metabolome of hexaploid wheat, leading to enhanced adaptability to salt, UV‐B, and alkali stress. ABSTRACT The hybridization‐led integration of the D‐genome into domesticated tetraploid wheat gave rise to allohexaploid wheat, the most cultivated wheat globally ...
Yanyan Liu, Tao Zhu, Ziru Zhou, Wei Chen, Chao He, Xinkai Zhou, Xin Wang, Chuanye Chen, Bo Yang, Jiaqi Wei, Caixia Lan, Mengmeng Liu, Handong Su, Qiang Li, Xin Hu, Siteng Bi, Weizhi Ouyang, Xingwang Li, Hailiang Mao, Masahiro Kishi, Kerstin Kaufmann, Alisdair R. Fernie, Dijun Chen, Wenhao Yan   +23 more
wiley   +1 more source

MOESM2 of Engineering a highly active thermophilic β-glucosidase to enhance its pH stability and saccharification performance

, 2016
Additional file 2. Circular dichroism spectrums of the wild type and mutant proteins of Bgl3A (0.5 mg/ml).
Xia, Wei, Xu, Xinxin, Qian, Lichun, Shi, Pengjun, Bai, Yingguo, Luo, Huiying, Ma, Rui, Yao, Bin   +7 more
openaire   +1 more source

Soil pH Amelioration Synergizes Carbon Accrual with Yield Gains Under Organic Substitution

Advanced Science, EarlyView.
Soil pH shifts towards neutrality in both acidic and alkaline soils under organic substitution. A global synthesis links pH amelioration to soil organic carbon accrual through enhanced microbial biomass after accounting for added organic carbon and its interaction with pH. This pH amelioration‐shared soil organic carbon accrual accounts for 5.1–8.6% of
Xunzhuo Dong, Yanzhong Yao, Bingbing Han, Yunyao Zhong, Benyi Li, Mengfei Li, Yunchao Dai, Zhaolei Li   +7 more
wiley   +1 more source

MOESM3 of Engineering a highly active thermophilic β-glucosidase to enhance its pH stability and saccharification performance

, 2016
Additional file 3. Primers used in this study.
Xia, Wei, Xinxin Xu, Lichun Qian, Pengjun Shi, Yingguo Bai, Huiying Luo, Ma, Rui, Yao, Bin   +7 more
openaire   +1 more source

β-Glucosidase activity from the thermophilic fungusScytalidium thermophilumis stimulated by glucose and xylose [PDF]

FEMS Microbiology Letters, 2004
An inducible mycelial beta-glucosidase from Scytalidum thermophilum was characterized. The enzyme exhibited a pI of 6.5, a carbohydrate content of 15%, and an apparent molecular mass of about 40 kDa. Optima of temperature and pH were 60 degrees C and 6.5, respectively.
Fabiana Fonseca, Zanoelo, Maria de Lourdes Teixeira de Moraes, Polizeli, Héctor Francisco, Terenzi, João Atílio, Jorge   +3 more
openaire   +2 more sources

Discovery of two novel β-glucosidases from an Amazon soil metagenomic library

, 2014
An Amazon soil microbial community metagenomic fosmid library was functionally screened for β-glucosidase activity. Contig analysis of positive clones revealed the presence of two ORFs encoding novel β-glucosidases, AmBGL17 and AmBGL18, from the GH3 and ...
D'haeseleer, Patrik, Heins, Richard, Simmons, Blake A., Costa, Ohana Yonara A., Singer, Steven, Gladden, John M., Bergmann, Jessica C., Quirino, Betania F.   +7 more
core   +1 more source

In vivo inhibition of α- and β-glucosidases in L4 larval Culex quinquefasciatus using no-choice exposure to conduritol β-epoxide.

, 2023
In vivo inhibition of α- and β-glucosidases in L4 larval Culex quinquefasciatus using no-choice exposure to conduritol β-epoxide.
Alden S. Estep (1858219), Edwin R. Burgess IV (12975484), Caitlin E. Taylor (3391622), Neil D. Sanscrainte (4775034), Lyle J. Buss (16898700)   +4 more
core   +1 more source