Results 101 to 110 of about 14,111 (183)
Zhongshan Daxue xuebao. Yixue kexue ban, 2009 【目的】 构建AFP启动子介导HSV-TK基因表达载体,并对其在肝癌细胞中特异性表达进行分析. 【方法】 采用PCR法扩增人AFP基因启动子?回收纯化后克隆入pBluescript II KDR-TK相同克隆位点,切取AFP-TK片段,然后通过酶联反应插入到pEGFP-C1中,构建成pEGFP-C1-AFP-TK、对获得的片段进行鉴定、细胞靶向和外源基因表达的鉴定。【结果】序列分析表明,该启动子含300 bp核苷酸,与已报道的序列比较,100%相符。限制性酶切分析,重组质粒pEGFP-C1-AFP ...
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[Neurospecific transmembrane protein 240 colocalizes with peroxisomes and activates Rho GDP dissociation inhibitor β]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoHu Q +7 more
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[The guidelines for diagnosis and treatment of chronic lymphocytic leukemia/small lymphocytic lymphoma in China (2025)]. [PDF]
Zhonghua Xue Ye Xue Za ZhiHematological Oncology Committee of China Anti-Cancer Association +2 more
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Zhongshan Daxue xuebao. Yixue kexue ban, 1996 选取５株ＨＣＶ共有序列合成一时５’端加端引物。上游加端为ＥｃｏＲⅠ切点，下游加端为ＢａｍＨＩ切点。以ＲＴ－ＰＣＲ扩增一长为５７８ｂｐ的ｃＤＮＡ作目的基因，相应酶切后，反向插入ＰＵＣ１８的多克隆位点，构建ｐＵＨＣ－Ｃ重组体。分别或混合应用ＨＣＶ特异内外引物和ｐＵＣ特异通用引物以ＰＣＲ扩增重组体，扩增产物分子片段均同预期一致。测序表明克隆基因与ＨＣＶ－ＣＨＮ最同源，在所测ＣＥ４９７个核苷酸及由之推导的１６０个氨基酸区域内。其核苷酸与氨基酸同源性都是９７．５％。
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[High expression of DTX2 promotes proliferation, invasion and epithelial-mesenchymal transition of oxaliplatin-resistant colorectal cancer cells]. [PDF]
Nan Fang Yi Ke Da Xue Xue BaoMa Z, Liu F, Zhao X, Zhang X.
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[Safety and efficacy of mitoxantrone liposome combined chemotherapy in the treatment of mixed phenotype acute leukemia]. [PDF]
Zhonghua Xue Ye Xue Za ZhiJiang HW +6 more
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