鼠白细胞介素-2 受体 α 、β链基因反义 RNA 真核表达质粒的构建
Zhongshan Daxue xuebao. Yixue kexue ban, 2000 【目的】构建鼠 IL-2R α与β 链基因的反义 RNA 真核表达质粒, 为反义 RNA 基因治疗细胞免疫相关疾病作准备。 【方法】用限制性内切酶从克隆载体上切下鼠 IL-2R α与β 链cDNA 及鼠 IL-2启动子, 克隆入真核表达质粒 pcDNA3 的多克隆 位点上,用酶切或 PCR 法鉴定正反连接。【结果】得到 4 个反向连接的重组质粒: pcAnti-mIL-2Rα , pcAnti-mIL-2Rβ ,pcAntim IL-2Rα β 及 pciAnti-mIL-2Rα β 。前三者由CMV ...
何承伟, 马涧泉
doaj
[The risk and management strategies for second primary tumor after CAR-T cell therapy]. [PDF]
Zhonghua Xue Ye Xue Za ZhiLi RN, Wang WY, Yang F, Zhang LL, Shi J.
europepmc +1 more source
, 2004 根据棉铃虫单核衣壳核多角体病毒(Helicoverpaarmigerasinglenucleocapsidnucleopolyhedrovirus,HaSNPV)fp25k基因的序列,设计引物,引入适当的酶切位点,利用PCR扩增出基因片段。将该基因片段克隆至原核表达载体pProEXHTb,经IPTG诱导,在大肠杆菌DH5α中获得了高效表达,表达产物的大小为32kDa。纯化蛋白产物免疫家兔制备抗血清。该抗血清可与原核表达的GST ...
吴东,邓菲,胡志红,袁丽,孙修炼
core
[Eculizumab resistance caused by C5 gene polymorphism in a patient with paroxysmal nocturnal hemoglobinuria: a case report]. [PDF]
Zhonghua Xue Ye Xue Za ZhiZhou RQ +5 more
europepmc +1 more source
, 2013 用电子克隆方法获得耐旱苔藓齿肋赤藓的热激蛋白60基因,采用生物信息学方法,对该基因编码蛋白从氨基酸组成、结构保守域、理化性质、信号肽、疏水性/亲水性、亚细胞定位、跨膜结构域、二级结构、功能域、活性位点、及同源性等方面进行了预测和分析.结果表明:齿肋赤藓热激蛋白60基因全长1841bp,开放阅读框1581bp,编码526个氨基酸残基；编码蛋白含有GroEL保守域,是chaperon-like superfamily家族；亚细胞定位分析显示,编码蛋白位于内质网中；活性化位点分析表明,编码蛋白存在6类活性位点；
GAO Bei +7 more
core
[KMT2A-rearranged acute lymphoblastic leukemia with lineage switch following relapse: a case report and literature review]. [PDF]
Zhonghua Xue Ye Xue Za ZhiQiao ZQ, Shi YP, Hu LD.
europepmc +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2008 【目的】 FAM3A (family with sequence similarity 3, member A)为新近发现的细胞因子FAM3的家族成员之一,本研究检测FAM3A的相互作用蛋白? 【方法】 克隆FAM3A并连接到pGB载体,转化酵母Y190宿主菌,加入含主要信号传导细胞因子人cDNA文库,按标准程序进行酵母双杂交,以X-Gal显色评估活性?提取扩增阳性克隆,连接pACT2载体,测序?检索基因库确定阳性克隆基因?克隆阳性基因并与FAM3A质粒共转染酵母进一步确定相互作用?
doaj
[Molecular characteristics for poor prognosis related renal cell carcinoma with lymph metastases]. [PDF]
Beijing Da Xue Xue Bao Yi Xue BanShu F +10 more
europepmc +1 more source
[Effects of pulsed electromagnetic fields on high-glucose-induced inhibition of HUVEC proliferation and its underlying mechanisms]. [PDF]
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za ZhiCao T +7 more
europepmc +1 more source
[Role and mechanism of infrared thermography in early identification of necrosis risk in random-pattern flaps of mice]. [PDF]
Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za ZhiJiang XQ +5 more
europepmc +1 more source