Results 11 to 20 of about 14,111 (183)
Veterinary Dermatology, Volume 34, Issue 4, Page 327-338, August 2023., 2023 Background – The caninised monoclonal antibody lokivetmab (LKV), directed at interleukin (IL)‐31, is very effective at controlling pruritus in most dogs with atopic dermatitis (AD). However, evidence exists that IL‐31 is not required for the induction of acute allergic skin inflammation, which might explain why this treatment is less efficacious in ...
Chie Tamamoto‐Mochizuki +4 more
wiley +1 more source
Factors that shape large‐scale gradients in clonality
Journal of Biogeography, Volume 50, Issue 5, Page 827-837, May 2023., 2023 Abstract Aim Many plant species reproduce clonally. However, ecologists still have much to learn about the factors that shape large‐scale patterns in plant clonal growth and reproduction, especially in the southern hemisphere. We addressed this knowledge gap by quantifying relationships between reproductive mode and a suite of plant characteristics and
Hongxiang Zhang +4 more
wiley +1 more source
General‐purpose genotypes and evolution of higher plasticity in clonality underlie knotweed invasion
New Phytologist, Volume 246, Issue 2, Page 758-768, April 2025.Summary Many widespread invasive plant species express high phenotypic variation across novel environments, providing a unique opportunity to examine ecological and evolutionary dynamics under global change. However, studies often lack information about the origin of introduced populations, limiting our understanding of post‐introduction evolution.
Shengyu Wang +15 more
wiley +1 more source
Long‐term use of lokivetmab in dogs with atopic dermatitis
Veterinary Dermatology, Volume 35, Issue 6, Page 683-693, December 2024.Background – Lokivetmab, a caninised monoclonal antibody against interleukin (IL)‐31, is an effective treatment for the pruritus associated with canine atopic dermatitis (cAD). Objectives – To investigate the efficacy and safety of lokivetmab during long‐term treatment defined as at least three consecutive lokivetmab injections in atopic dogs under ...
Bettina Kasper +2 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2000 【目的】构建人瘦素基因 cDNA 克隆, 并进行序列分析。【方法】用逆转录聚合酶链反应扩增人瘦素基因 cDNA , 获得片段( 640 bp)连接至 pUC19 载体, 并转化大肠杆菌 DH5α。以末端终止法进行序列测定。【结果】所克隆的人瘦素 cDNA 含全长的人瘦素基因编码区,仅 287 位的腺苷酸被鸟苷酸代替, 导致 96 位编码谷氨酰胺的密码子 CAA 改变为编码精氨酸的 CGA, 其意义尚待阐明。【结论】以逆转录聚合酶链反应方法成功地构建了人瘦素基因 cDNA 克隆。
徐明彤, 钟光恕, 傅祖植
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野生动物学报, 2007 根据GenBank上已发表的H6N2亚型的非结构蛋白基因（NS）序列,设计一对特异性引物,成功从本实验室分离、保存的H6N2禽流感病毒分离株A／Mallard／SanJiang/151／06（H6N2）中克隆到NS基因序列。该克隆基因全长860bp,编码NS1和NS2两种非结构蛋白,与其他H6N2亚型的NS基因进行同源性比较,同源性为71．1％～97．2％,推导的氨基酸序列同源性为62．0％～94．6％。
张智明, 李晓冰, 华育平
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Zhongshan Daxue xuebao. Yixue kexue ban, 2001 【目的】克隆人地中海贫血基因β654, 并进行序列分析, 为下游转基因小鼠模型的建立奠定工作基础。【方法】 以PCR 法扩增获得人β654 基因, 将其克隆入卸除了人β 基因的质粒pBGT51 中, 酶切、反向点杂交及测序法鉴定重组质粒。 【结果】所构建的重组质粒中含人β654 基因, 其引入方向正确, 序列分析结果正确。【结论】构建所得的重组质粒可用于下游 的转基因工作。
方小武, 曾瑞萍, 胡 彬
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Zhongshan Daxue xuebao. Yixue kexue ban, 1992 从两例萄糖一6一磷酸脱氢酶变异型——台湾客家型[Gd（-）Taiwan-Hakka]患者基因组DNA中,回收经EcORⅠ完全酶解的3～5 kb片段,与载体λgt10插入重组,用SMR10单菌种系统制备包装提取物,对重组的λgt10进行体外包装,构建成两个基因组基因文库。用32P中标记的G6 PDcDNA成功地从文库中初选出12个阳性克隆,挑选其中4个阳性克隆再次杂交予以证实,提取其中2个克隆DNA,经限制酸酶切电泳及斑点杂交鉴定,分离到该变异型基因的插入片段,并将其克隆到M13 mP19噬菌体,完成Gd（
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