Results 41 to 50 of about 1,341,470 (171)

Local Public Goods Provision in the Post-Agricultural Tax Era in Rural China [PDF]

open access: yes
This paper investigates regional differences in local public goods provision in rural area in the 2000s, using large village sample surveys (CHIP 2002 and 2007 surveys, a survey in Ningxia).
Hiroshi Sato, Sai Ding
core  

Environmental Justice in the Brazilian Coastal Food Systems: Policies, Institutions, and Knowledges on Small‐Scale Fishers and Energy Development

open access: yesWorld Food Policy, Volume 12, Issue 2, November 2026.
ABSTRACT This study explores environmental justice issues in coastal food systems, focusing on conflicts between small‐scale fisheries and the energy sector due to differing goals in managing food and energy sources. It does so by examining the interdependence between institutions, policies, and different types of knowledge; key elements in building ...
Mateus Henrique Amaral   +6 more
wiley   +1 more source

基因组叠加生物型的基因表达特征

open access: yes, 2000
采用聚丙烯酰胺梯度凝胶电泳及特异性组织化学染色技术分析了鲤、鲫及其基因组叠加的不同组合个体的红细胞SOD. EST和血红蛋白的电泳图谱。结果表明不同基因组组合及不同倍性的个体间存在由基因组差异导致的生化多态性,为不同倍性的鱼类的同工酶(SOD)表达存在基因剂量效应提供了依据,证实了不同组合的SOD亚基间有协同表达、累积作用。研究表明每个基因有其独特的调控机制,因而在杂种三倍体内三分之一的基因组亦能够表达产物。应用本研究的遗传学数据有益于构建基因组特异性品系 ...
吴玉萍,叶玉珍,吴清江
core  

AS-PCR法检测CLU基因SNP位点方法的建立

open access: yesZhongguo shiyan zhenduanxue, 2019
目的基于等位基因特异性PCR(allele-specific PCR,AS-PCR)原理,建立一种QPCR检测CLU基因与阿尔兹海默病(AD)相关性SNP位点基因型的筛选方法。方法根据CLU基因上的rs11136000,rs2279590和rs9331888 3个SNP位点设计特异性的野生型、突变型引物,使用实时荧光定量PCR(QPCR)对AD患者外周血样本进行SNP基因分型,以DNA基因测序法结果作为参考标准。结果 10例样本的QPCR定量分型结果显示 ...
李逸豪   +8 more
doaj  

Farm‐Scale Recharge Pits for Improving Local Groundwater Availability: Insights From a Hardrock Watershed in Rajasthan, India

open access: yesWorld Water Policy, Volume 12, Issue 3, August 2026.
ABSTRACT Groundwater depletion is a major challenge in semiarid, hard‐rock regions where irrigation demands often exceed natural recharge. This study evaluates the effectiveness of farm‐scale recharge pits as a local, supplementary, and community‐managed groundwater recharge solution in the Dharta catchment of Rajasthan, India. Alongside check dams and
Prahlad Soni   +4 more
wiley   +1 more source

转pMThGH基因红鲤F_4代鱼体内转植基因存在的分子多态性

open access: yes, 2000
从一尾转pMThGH基因红鲤F4代仔鱼基因组内回收并克隆了50个转植基因(transgene),对其中33个进行了分析.根据双酶切结果,可将这些回收基因分为4种类型.用5种内切酶构建了这 4种类型回收基因的限制性内切酶图谱,第 1种类型(6个,占 18%)与建立原代转基因鱼所用的外源基因相同,而其他3种类型回收基因的分子量大小、酶切位点的种类、数量、位置都迥异于转移前的结构,意味着大多数转植基因顺序发生了缺失和重排.Southern杂交进一步证明了这一结果.对回收基因侧翼进行顺序分析 ...
曾志强,朱作言
core  

Justice in coexistence: Pastoralism and large carnivores on the Qinghai‐Tibet Plateau

open access: yesPeople and Nature, Volume 8, Issue 7, Page 2186-2199, July 2026.
Abstract The conflict between livestock husbandry and large carnivore conservation presents significant challenges in grassland ecosystems worldwide. Achieving sustainable coexistence among herders, livestock and large carnivores requires reconciling diverse perspectives and interests through equitable, inclusive and fair processes that address both ...
Yufang Gao, Yue Yu
wiley   +1 more source

过氧化物酶增生物-激活受体γ2基因多态性与2型糖尿病的相关性

open access: yesZhongguo shiyan zhenduanxue, 2010
目的探讨PPARγ2基因多态性与2型糖尿病的关系。方法以rs1875796为遗传标记,应用多聚酶链-限制性片段长度多态性(PCR-RFLP)技术检测250例2型糖尿病患者和337例健康对照人群PPARγ2基因型。结果 Rs1875796基因型频率CC:CT:TT在2型糖尿病和正常对照组的频率分布分别为2.8%:27.2%:70.0%和2.7%:25.5%:71.8%;C等位基因和T等位基因在2型糖尿病组和正常对照组的频率分布分别为16.4%:83.6%和15.4%:84.6%,两组间的等位基因 ...
高鹏, 吴江, 杜丹华, 张颖
doaj  

不同基因型小麦水分、养分利用效率的比较研究

open access: yes, 2003
水分亏缺和土壤肥力低下是限制旱地小麦生产的两个重要因素。在有限的水分和养分的条件下,提高小麦自身的水分和养分利用效率是解决这一问题的关键。一些报道证明,水分和养分供应对小麦的水分、养分利用效率有显著作用。目前的研究多以现代栽培品种为材料,有可能遗失了一些古老品种的优良特性。为此本文选取了6个在进化中典型的基因型小麦为材料,在不同水肥条件下对其水分、养分利用效率及相关生长、生理指标进行了测定,以探索小麦水分、养分利用的进化关系和水肥条件对它们的影响。主要结果如下:    1.
黄明丽
core  

东北汉族人群抵抗素基因rs1862513(G/C)多态性与2型糖尿病相关性研究

open access: yesZhongguo shiyan zhenduanxue, 2017
目的探讨东北汉族抵抗素基因rs1862513(G/C)位点与2型糖尿病发病及临床表型相关性。方法应用连接酶反应分型法检测79例2型糖尿病患者及79例正常对照者rs1862513(G/C)多态性。结果 2型糖尿病组和对照组抵抗素rs1862513(G/C)位点基因型分布分别为GG49.5%、GC44.3%、CC6.2%和GG40.2%、GC47.4%、CC12.4%;等位基因频率分别为G型71.6%、C 28.4%和G型63.9%、C型36.1%,该位点的基因型分布和等位基因频率在两组间分布均无统计学意义(
陈珍珍   +4 more
doaj  

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