Results 41 to 50 of about 26,684 (165)
Zhongguo shiyan zhenduanxue, 2015 目的调查疾病易感性相关的髓过氧化酶（MPO）、人类8-羟基鸟嘌呤糖苷酶（hOGG1）、锰超氧化物歧化酶（MnSOD）基因多态性在韩国人群中的分布状况。方法采用聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）技术分别分析300名韩国健康男女大学生的MPO基因启动子区-463bp位点、hOGG1基因第7外显子第1245位碱基C/G、MnSOD基因1183位点T/C的单核苷酸多态性—M-4,计算基因型和基因频率。结果 MPO基因型频率为G/G型80.3%、G/A型19.0%、A/A型0.7 ...
卢焕俊, 洪润哲, 许青松
doaj
Partisanship, Deservingness, and the Attitudinal Policy Feedback Process for Social Policy
Policy Studies Journal, EarlyView.ABSTRACT In an era of identity‐based partisan polarization, we examine whether social policies can still generate positive attitudinal feedback among beneficiaries. Drawing on nationally representative survey data, we demonstrate that partisanship conditions the policy feedback process through divergent perceptions of group deservingness.
Chris Faricy, Christopher Ellis
wiley +1 more source
Development of Real-time PCR Genotyping Methods Based on Probe Coding Technology [PDF]
, 2006 本论文围绕探针编码技术基因分型展开了研究，研究工作主要包括探针编码技术的提出和基本原理，以及探针编码技术在三种不同研究对象基因分型中的应用。第一章，提出了一种用于基因分型的新型探针编码技术，并对其基本原理和设计规则进行了系统的说明。探针编码技术通过不同荧光染料的组合标记靶序列，利用实时PCR过程中采集到的荧光信号来判断基因型。它可以超越实时PCR仪器检测通道的限制，具有高通量，简单快速，特异性强等优点，极大地提高了实时PCR基因分型的能力。第二章，以食源性致病菌蜡样芽孢杆菌为研究对象 ...
郑琳琳
core
Zhongguo shiyan zhenduanxue, 2011 目的分析大肠埃希菌、肺炎克雷伯菌临床分离株中超广谱β内酰胺酶（ESBL）的基因型分布情况。方法用表型确证试验确定临床标本中产ESBL的大肠埃希菌和肺炎克雷伯菌分别用TEM、SHV和CTX-M通用引物进行聚合酶链反应（PCR）扩增,鉴定其基因型。结果 66%的菌株为TEM基因型,14%的菌株为SHV基因型,10%的菌株为CTX-M型。结论大肠埃希菌、肺炎克雷伯菌临床分离株中超广谱β内酰胺酶（ESBL）的基因型分布以TEM基因型为主,部分菌株为SHV、CTX-M型。同一菌株内可以产生2种不同基因型的超广谱β-
黄晶, 许建成, 安倍莹, 白岩
doaj
Political Cycles, Electricity Policy, and Mexico's Economy
Latin American Policy, Volume 17, Issue 2, June 2026.ABSTRACT Electricity reform in Mexico should stimulate economic growth, improve energy efficiency, and reduce greenhouse gas emissions. This article evaluates the successes and failures of reform and draws lessons across political cycles by analyzing changes in political dynamics—often tied to policy reversals—and the legal framework during the pro ...
David Bonilla
wiley +1 more source
The study about the association of miRNA1269a SNP and the susceptibility to hepatocellular carcinoma [PDF]
, 2016 研究背景及目的： 基于早期肝癌患者缺乏特异症状和体征，而中晚期肝癌患者治疗效率又比较低并且预后较差，探讨肝癌的早期诊断与其发生机制具有重要意义。miRNA是一类由内源基因编码的长度约为22个核苷酸的非编码单链RNA分子，它们在动植物中参与转录后基因表达调控，其形成过程从长链pri-miRNAs到pre-miRNAs，最后形成成熟miRNAs，可直接结合到mRNA的3’UTR，从而抑制mRNA的翻译，导致mRNA的降解，进而影响蛋白质的表达，改变生物过程 ...
闵培
core
Zhongguo shiyan zhenduanxue, 2007 目的评价基因芯片技术在HBV基因型及HBVP基因YMDD变异检测中的应用价值,并对不同基因型病例的临床情况进行分析。方法抽提HBV DNA,用PCR方法扩增S基因及P基因部分区域,扩增产物与基因芯片上的寡核苷酸探针进行杂交,杂交结果通过芯片影像读取仪扫描到计算机上,经软件分析可得到HBV基因型、HBVYMDD野生型及YVDD、YIDD变异型结果;部分标本通过HBV preS/S基因序列测序证实。结果38份高病毒滴度标本（HBV DNA定量>1.0×105拷贝/毫升）中22例为B基因型（58 ...
陈伟烈 +5 more
doaj
Veterinary Dermatology, Volume 37, Issue 3, Page 353-364, June 2026.ABSTRACT Background Canine atopic dermatitis (cAD) is a multifactorial, inherited skin disease, estimated to affect ≤ 15% of dogs. Studies of skin messenger mRNA in cAD currently use invasive methods, including blood sampling and biopsy collection, whilst advances in human atopic dermatitis study methodology have demonstrated reliable use of minimally ...
Xavier Langon +5 more
wiley +1 more source
陕西省87株耐药结核分枝杆菌寡核苷酸基因分型和耐药性分析 [PDF]
, 2018 目的对陕西87例耐药结核分枝杆菌进行分型研究,了解不同基因型在陕西的流行情况及耐药相关性。方法收集陕西省结核分枝杆菌耐药菌株87株,采用Spoligotyping方法对结核分枝杆菌临床分离株进行基因分型。结果 87株耐药结核分枝杆菌中北京家族占86.21%(75/87),非北京家族占13.79%(12/87);非北京基因型中,T家族(T1、T2、T2-3、T3)占9.20%(8/87),Manu家族占1.15%(1/87),新发现基因型占3.45%(3/87)。北京家族和非北京家族的单耐药、多耐药 ...
张天华 +5 more
core
ABCA12 Frameshift Deletion in Domestic Cats With Ichthyosis Fetalis
Veterinary Dermatology, Volume 37, Issue 3, Page 365-374, June 2026.ABSTRACT Background Ichthyosis fetalis (IF), also known as harlequin ichthyosis, is a rare and often fatal autosomal recessive congenital skin disorder. It is characterized by thickened, hard skin plaques and deep skin fissures that limit mobility and cause malformations of the eyes, lips and ears.
Jeanna M. Blake +2 more
wiley +1 more source