Results 61 to 70 of about 29,153 (221)
Importance of connectivity for carnivore richness and occupancy in fragmented biodiversity hotspots
Conservation Biology, Volume 39, Issue 6, December 2025.Abstract Structural connectivity affects wildlife movement between habitat patches, contributing to the persistence of wildlife populations and their resilience to human‐induced and environmental changes. However, its importance to wildlife population persistence remains unclear, particularly in fragmented landscapes, where there are additional co ...
Cindy M. Hurtado +12 more
wiley +1 more source
PCR-SSP对潜在输血需要住院患者ABO基因分型的检测效果与意义
Zhongshan Daxue xuebao. Yixue kexue ban, 2013 【目的】 调查潜在输血需要住院患者的ABO血型基因,分析ABO表型相同输血中ABO基因型的错配输血几率。【方法】 对502例潜在输血需要的住院患者,采用血清学方法和聚合酶链反应-序列特异性扩增技术(PCR-SSP)检测ABO血型的表型及基因型。比较两种方法学对ABO血型表型的一致率;统计各ABO等位基因及各ABO基因型的频率;并分析潜在输血需要的住院患者在接受ABO血型表型相同的输血时,ABO基因错配输血几率。 【结果】 血清学与PCR ...
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The study about the association of miRNA1269a SNP and the susceptibility to hepatocellular carcinoma [PDF]
, 2016 研究背景及目的： 基于早期肝癌患者缺乏特异症状和体征，而中晚期肝癌患者治疗效率又比较低并且预后较差，探讨肝癌的早期诊断与其发生机制具有重要意义。miRNA是一类由内源基因编码的长度约为22个核苷酸的非编码单链RNA分子，它们在动植物中参与转录后基因表达调控，其形成过程从长链pri-miRNAs到pre-miRNAs，最后形成成熟miRNAs，可直接结合到mRNA的3’UTR，从而抑制mRNA的翻译，导致mRNA的降解，进而影响蛋白质的表达，改变生物过程 ...
闵培
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Conservation Biology, Volume 39, Issue 6, December 2025.Abstract As the coverage of area‐based conservation increases across the globe, it is critical to improve understanding of the social and ecological outcomes of such measures and the pathways to their outcomes. A social–ecological systems approach to monitoring and evaluation is increasingly advocated; yet, applications remain scarce.
Natalie C. Ban +21 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2009 【目的】 研究ABO血型基因编码区全长序列及对疑难标本的初步应用65377;【方法】 对6份已知ABO血型的血样进行DNA抽提,针对ABO基因启动子65380;所有外显子65380;部分内含子序列设计65380;合成一系列特异性引物,探索它的最佳PCR反应体系和扩增条件,获得的PCR产物直接进行序列测定65377;用建立的方法对2份ABO疑难血型的标本进行全长序列测定65377; 【结果】 得到ABO基因中A10165380;B65380;O0165380;O0265380 ...
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Zhongshan Daxue xuebao. Yixue kexue ban, 1997 摘 要 建立泌尿生殖系统沙眼衣原体感染阳性标本直接基因分型方法。先用敏感的扩增沙眼衣原体特异质粒 的引物扩增500份临床标本, 初筛选出100份阳性标本再用扩增沙眼衣原体主要外膜蛋白( MOM P)基因的引物扩 增, 并用嵌套式PC R使阳性标本能扩增出MOMP基因, 对获得的MOMP基因用限制性酶切片段长度多态性 ( RFLP)方法进行基因分型,并用银染观察结果。其中E型比例最高,占47% ; F型为16% ; Da、G型均为6% ; Ba、D 型各占5% ; H型及K型均为1% ,混合型K /
汪玎妍 郭辉玉 罗宪玲 蒋文玲 李质怀
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Analysis of genotypes and biochemical phenotypes of neonates with abnormal metabolism of butyrylcarnitine. [PDF]
Zhejiang Da Xue Xue Bao Yi Xue Ban, 2023 Wu D +6 more
europepmc +1 more source
, 2012 利用泥鳅(Misgurnus anguillicaudatus)的微卫星引物跨种扩增大鳞副泥鳅(Paramisgurnus dabryanus),通过家系扩增、PCR产物测序筛选出6个座位,用于大鳞副泥鳅7个野生群体的遗传结构分析。结果显示,群体的平均等位基因数在3.167～4.833,平均观测杂合度(Ho)在0.248～0.417,平均期望杂合度(He)在0.379～0.505,多个座位存在零等位基因、杂合子缺失现象,显示大鳞副泥鳅种群遗传多样性较低。采用UPGMA法对7个群体基于DA遗传距离进行聚类,
俞小牧, 游翠红, 童金苟
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Veterinary Dermatology, Volume 36, Issue 6, Page 862-869, December 2025.ABSTRACT Background Chronic cases of canine otitis externa (OE) often develop infections with Pseudomonas aeruginosa (PA). Given the organism's high level of resistance, veterinary surgeons often turn to compounded solutions. Limited data describing the stability and potency of compounded ceftazidime (CAZ) solutions are available, which may affect ...
McKenna Snidow +6 more
wiley +1 more source
肾素－血管紧张素系统基因多态性与冠心病合并慢性心力衰竭的关系
Zhongshan Daxue xuebao. Yixue kexue ban, 2008 【目的】探讨中国南方部分汉族人群的冠心病患者中，肾素－血管紧张素系统中的关键成分即血管紧张素转换酶(ACE)及血管紧张素原（AGT）双基因多态性与冠心病合并慢性心力衰竭（CHF）发病的关系。【方法】 应用聚合酶链反应及限制性片段长度多态性技术，对215例冠心病患者的ACE基因插入/缺失(I/D)及AGT M235T多态性进行检测。将其中105例合并CHF者作为病例组，其余110例心功能正常者作为对照组。【结果】①冠心病合并CHF组DD基因型及D等位基因的频率均高于对照组（前者为45.7％vs23.6 ...
牛云茜 +5 more
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