Results 61 to 70 of about 34,083 (145)

拉米夫定耐药感染者HBV基因与YMDD变异的相关性探讨

open access: yesZhongguo shiyan zhenduanxue, 2013
目的探讨拉米夫定耐药感染者HBV基因型与YMDD变异的相关性。方法采用PCR扩增后测序的方法,对162例拉米夫定耐药感染者进HBV基因型的分析。实时荧光定量PCR法检测HBV YMDD变异及HBV DNA载量。率的比较采用χ2检验法,两组均数间的比较采用t检验法。结果 162例拉米夫定耐药感染者中,B基因型102例(63.0%),C基因型60例(37.0%)。YMDD变异111例(68.5%),其中YIDD变异30例(18.5%),YVDD变异32例(19.7%),YIDD/YVDD混合型变异49例 ...
张志珊   +6 more
doaj  

Climate stability drives multidimensional nestedness of amphibian assemblages in a Chinese sky island system

open access: yesFunctional Ecology, Volume 40, Issue 3, Page 636-654, March 2026.
Read the free Plain Language Summary for this article on the Journal blog. Abstract The nested subset pattern (nestedness) has been widely used to explain species distributions in island and fragmented systems. Mountain sky islands serve as critical natural laboratories for understanding the evolutionary consequences of geographic isolation and climate
Caiwen Zhang   +5 more
wiley   +1 more source

东北汉族人群抵抗素基因rs1862513(G/C)多态性与2型糖尿病相关性研究

open access: yesZhongguo shiyan zhenduanxue, 2017
目的探讨东北汉族抵抗素基因rs1862513(G/C)位点与2型糖尿病发病及临床表型相关性。方法应用连接酶反应分型法检测79例2型糖尿病患者及79例正常对照者rs1862513(G/C)多态性。结果 2型糖尿病组和对照组抵抗素rs1862513(G/C)位点基因型分布分别为GG49.5%、GC44.3%、CC6.2%和GG40.2%、GC47.4%、CC12.4%;等位基因频率分别为G型71.6%、C 28.4%和G型63.9%、C型36.1%,该位点的基因型分布和等位基因频率在两组间分布均无统计学意义(
陈珍珍   +4 more
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From Presence to Policy: The Conditional Impact of Women's Representation in Top Management Teams in Chinese Local Governments

open access: yesPublic Administration, Volume 104, Issue 1, Page 175-187, March 2026.
ABSTRACT While public administration research has begun exploring the impact of women's representation in top leadership positions in public organizations, the conditions under which women's representation in top management teams (TMTs) produces policies specifically benefiting women remain underexamined.
Ting Huang   +3 more
wiley   +1 more source

HBV-DNA高水平孕妇的HBV 前S和S区基因突变及基因分型

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2012
【目的】 研究HBV-DNA高水平孕妇的HBV前S(前S1和前S2)及S区的基因突变模式和基因分型,为从病毒因素探讨HBV母婴传播免疫阻断失败提供实验基础【方法】 选取血清HBsAg阳性且HBV-DNA≥105 IU/ mL的孕妇41 例,提取血清HBV-DNA,靶向设计HBV各基因型的前S及S区通用引物, 经PCR 扩增测序进行序列分析, 检测和分析前S及S区基因的突变情况,并进行基因分型【结果】 ①在 41 例样本中, 共有29例( 70.73%)样本存在前S1前S2及S区基因突变 ...
doaj  

成骨细胞条件性FKBP8基因敲除小鼠模型构建

open access: yesZhongguo shiyan zhenduanxue, 2019
目的建立成骨细胞条件性FKBP8基因敲除小鼠模型、为研究FKBP8基因在骨质疏松症中的作用机制提供动物模型。方法设计基因敲除策略,获得纯合子小鼠和野生型对照小鼠。提取鼠尾DNA,PCR扩增之后琼脂糖凝胶电泳鉴定小鼠基因型。提取小鼠原代成骨细胞蛋白质,利用Western blot技术检验FKBP8基因的表达。结果成功构建成骨细胞条件性FKBP8小鼠敲除模型,PCR结果显示基因型为BGLAP-Cre/FKBP8flox/flox,Western blot结果显示FKBP8基因蛋白表达敲除效果明显 ...
王红   +4 more
doaj  

Survey Experiments in Public Policy: A Systematic Literature Review

open access: yesReview of Policy Research, Volume 43, Issue 2, March 2026.
ABSTRACT This systematic literature review examines population‐based survey experiments (PBSE) in public policy, analyzing 36 peer‐reviewed articles from Web of Science and Scopus databases. Based on PBSE methodological literature and the review, four main types of PBSE are distinguished: methodological, direct (based on information provision ...
Radek Kovács, Arnošt Veselý
wiley   +1 more source

广州地区丙型肝炎患者病毒基因分型 及其与临床病情的关系

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 1999
目的: 探讨广州地区丙型肝炎病毒(HCV)基因型分布的特点以及 HCV 基因型与临床病情的关系。方法: 病例 选自广州居民,基因分型使用 Okamoto 的“型特异性引物PCR 法” ,并加以改进,基因型的命名采用国际上统一的 Simmonds两 级命名体系。结果: 广州 HCV 基因型分布 1a 占 7. 6%、1b 占 49. 5%、2a 占 23. 8%、2b占 1. 9%、3a 占 2. 9%、1b+2a 混合型占 9.
安 阳 杨绍基 姚集鲁
doaj  

PCR-SSP对潜在输血需要住院患者ABO基因分型的检测效果与意义

open access: yesZhongshan Daxue xuebao. Yixue kexue ban, 2013
【目的】 调查潜在输血需要住院患者的ABO血型基因,分析ABO表型相同输血中ABO基因型的错配输血几率。【方法】 对502例潜在输血需要的住院患者,采用血清学方法和聚合酶链反应-序列特异性扩增技术(PCR-SSP)检测ABO血型的表型及基因型。比较两种方法学对ABO血型表型的一致率;统计各ABO等位基因及各ABO基因型的频率;并分析潜在输血需要的住院患者在接受ABO血型表型相同的输血时,ABO基因错配输血几率。 【结果】 血清学与PCR ...
doaj  

儿童血清25羟基维生素D水平及其基因多态性与哮喘的相关性研究

open access: yesZhongguo shiyan zhenduanxue, 2015
目的通过对哮喘患儿血清中血清25羟基维生素D水平及维生素D受体基因多态性分析,拟探讨25-(OH)-VD在哮喘发病中的作用。方法本院门诊随诊的40例哮喘患儿为哮喘组,健康体检的儿童40例为对照组。采用酶联免疫吸附试验检测两组患儿血清25羟基维生素D水平,采用DNA测序技术检测两组患儿VDR基因位点rs2228570、rs7975232、rs11574114、rs1544410、rs731236、rs757343有无多态性存在。结果哮喘组儿童血清25-(OH)-VD水平均低于正常对照组,差异有统计学意义 ...
赵宏霞   +3 more
doaj  

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