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The Genome of Phoebe zhennan (Lauraceae) and Comparative Genomics of Phoebe 楠木(樟科)基因组与楠属植物比较基因组学研究
Integrative Conservation, Volume 4, Issue 4, Page 584-599, December 2025.Phoebe zhennan is a Chinese endemic and valuable timber species of Phoebe (Lauraceae). We present a chromosome‐level genome assembly of P. zhennan. Our research provides novel insights into the genome evolution of Phoebe, including whole‐genome duplication, phylogenetic relationships of magnoliids, and key MYB regulatory genes involved in lignin ...
Mao Yameng +3 more
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重组腺病毒介导RECK基因在大肠癌细胞中的表达及抑制癌细胞浸润能力的实验研究
Zhongguo shiyan zhenduanxue, 2005 目的 研究重组腺病毒介导RECK基因转染大肠癌细胞后基因的表达情况,并观察转染细胞浸润能力变化。方法 构建了RECK基因的复制缺陷型重组腺病毒载体adRECK ,以adRECK转染大肠癌癌细胞株后,用RT PCR法检测RECK基因在大肠癌中的表达。并用Boyden小室测试转染细胞的侵袭性。结果 RECK基因在转染细胞中有效的表达。被转染细胞浸润能力明显下降。结论 重组腺病毒介导RECK基因能够在大肠癌细胞中有效的表达,转染病毒的大肠癌浸润能力明显下降。
杨德君 +4 more
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Veterinary Dermatology, Volume 36, Issue 6, Page 862-869, December 2025.ABSTRACT Background Chronic cases of canine otitis externa (OE) often develop infections with Pseudomonas aeruginosa (PA). Given the organism's high level of resistance, veterinary surgeons often turn to compounded solutions. Limited data describing the stability and potency of compounded ceftazidime (CAZ) solutions are available, which may affect ...
McKenna Snidow +6 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】探讨腺病毒介导血管内皮细胞生长因子(VEGF165)基因转染大鼠骨髓间充质干细胞(MSCs)后目的基因表达情况及对MSCs增殖分化的影响。【方法】构建含VEGF基因的腺病毒表达载体,利用贴壁法分离培养MSCs后,用荧光显微镜和流式细胞仪检测转染效果和转染率,用免疫组化、Western-Blot和ELISA方法分别检测VEGF基因转染MSCs后VEGF的表达情况。【结果】腺病毒介导的VEGF基因对于MSCs具有很高的转染效率,转染效率与病毒感染复制数(multiplicyties of ...
华平 +6 more
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Veterinary Dermatology, Volume 36, Issue 6, Page 723-787, December 2025.Hyperkeratosis of (a) the footpads and (b) the nasal planum. ABSTRACT Background Canine leishmaniosis (CanL) due to Leishmania infantum remains common, and veterinarians do not always follow scientifically sound approaches for diagnosis, treatment and prevention. Objectives To provide consensus guidelines for diagnosis and evidence‐based guidelines for
Manolis N. Saridomichelakis +9 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2012 目的探讨klotho基因转染对脂肪间充质干细胞（ADSCs）增殖的影响。方法将含有klotho全长基因的真核质粒转染至从脂肪组织分离的ADSCs中,采用CCK-8法测定转染后细胞增殖率,western blot法检测转染后细胞周期蛋白D1的表达变化,流式细胞术检测转染前后ADSCs的细胞周期变化。结果转染klotho基因96h后AD-SCs的细胞增殖率为170.50%;流式细胞术检测结果显示,ADSCs转染klotho基因S期和G2期细胞明显增多;west-ern ...
韩冬, 徐煌, 郑永霞, 肖燕萍
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Ultrasound in Obstetrics &Gynecology, Volume 66, Issue 5, Page 582-588, November 2025.ABSTRACT Objective The corpus callosum (CC) is an interhemispheric structure that facilitates communication between the two cerebral hemispheres. Anomalies of the CC are frequent and associated with a wide spectrum of altered neurodevelopmental outcomes.
R. Corroenne +55 more
wiley +1 more source
Zhongshan Daxue xuebao. Yixue kexue ban, 2004 【目的】构建神经营养素-3(NT-3)基因转染的施万细胞。【方法】NT-3基因重组腺病毒(AdvNT-3)扩增、纯化和测定后,转染体外培养的施万细胞(SCs)。用免疫细胞化学和ELISA方法分别检测NT-3基因转染SCs的NT-3表达及培养液中NT-3的含量。【结果】实验中获得的AdvNT-3中外源基因序列与大鼠NT-3的DNA序列完全一致,与未基因转染SCs相比,NT-3基因转染SCs的NT-3阳性增强,培养液中NT-3含量增高。【结论】通过腺病毒介导可以获得NT-3过量表达的基因转染SCs。
郭家松
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New Phytologist, Volume 247, Issue 5, Page 2086-2097, September 2025.Summary The importance of marine angiosperms, or seagrasses, as foundation species for marine coastal ecosystems is in marked contrast to their low species number of only 70 described taxa. Seagrass species tend to have very similar overall morphologies dictated by hydrodynamic forces of the ocean environment.
Xiaomei Zhang +14 more
wiley +1 more source
Sox9基因转染诱导L6大鼠成肌细胞向软骨细胞表型分化的研究
四川大学学报. 自然科学版, 2011 利用脂质体转染法,将真核表达质粒pcDNA3.1-Sox9导入L6细胞中.通过细胞增殖检测、细胞形态学观察、标志基因表达分析和甲苯胺蓝染色等方法分析Sox9基因转染对L6细胞增殖和向成软骨方向分化的诱导作用.结果表明,Sox9基因转染对L6细胞的增殖能力没有影响,但能明显抑制L6细胞相互融合形成肌管.和对照相比,Sox9基因转染后,细胞成肌分化标志基因Myf5的表达受到明显抑制,而成软骨分化标志基因Ⅱ型胶原(typeⅡcollagen,Col2a1)和蛋白聚糖(Aggrecan,Agg)的表达显著提高 ...
宋宏梅 +4 more
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