Results 101 to 110 of about 1,252 (143)

人神经生长因子基因真核细胞表达载体的构建与鉴定

Zhongshan Daxue xuebao. Yixue kexue ban, 1999
目的: 构建携带人神经生长因子( hNGF)基因的真核细胞表达载体, 为应用 NGF 进行老年性痴呆(AD)等疾病基 因治疗打基础。方法: 应用基因重组技术将 hNGF cDNA 克隆到逆转录病毒载体 pLXSN 中 ,用限制性内切酶酶切分析重组质粒 pLNGFSN 中 hNGF 基因的插入方向,用 PCR、斑点杂交和 Southern 杂交对重组质粒 pLNGFSN 作进一步鉴定。结果: hNGF cDNA 已正确地克隆到逆转录病毒载体 pLXSN 中,而构建成重组逆转录病毒载体 pLNGFSN ...
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[Preparation of polycaprolactone-polyethylene glycol-concentrated growth factor composite scaffolds and the effects on the biological properties of human periodontal ligament stem cells]. [PDF]

Hua Xi Kou Qiang Yi Xue Za Zhi
Gao L, Zhao M, Yang S, Wang R, Cheng J, Chen G.   +5 more
europepmc   +1 more source

表达人癌胚抗原重组痘苗病毒的构建

Zhongshan Daxue xuebao. Yixue kexue ban, 1997
摘　要　用痘苗病毒作载体,成功构建了高效表达人癌胚抗原( CEA)的CE A-重组痘苗病毒, 用143 TK-细 胞作为宿主细胞,表达的蛋白质只在细胞膜上能检测到,而胞质内和细胞上清液中均未能检测到, 可见所表达 的蛋白质保持了其原有特性,再次证实痘苗病毒是真核表达的有效载体。本结果为进一步研究CE A-重组痘苗 病毒能否激活机体的免疫系统杀伤表达C EA的肿瘤提供了物质基础。
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[Mechanisms and protective strategies for astronaut skin injury in deep space environments]. [PDF]

Zhong Nan Da Xue Xue Bao Yi Xue Ban
Xie Y, Zeng J.
europepmc   +1 more source

[Extracellular matrix materials and tissue regeneration and repair]. [PDF]

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
Zhao L, Xie H.
europepmc   +1 more source

[Structural design and mechanical analysis of a "drum-shaped" balloon-expandable valve stent in expanded configuration]. [PDF]

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
Zhao Y, Hou Q, Zhou J, Chen S, Zhang H, Qiao A.   +5 more
europepmc   +1 more source

[Advances in the application strategies of CRISPR/Cas9 technology in chimeric antigen receptor T cell therapy for hematological malignancies]. [PDF]

Zhonghua Xue Ye Xue Za Zhi
Wang YW, Tang YM.
europepmc   +1 more source

[Metabolic reprogramming by glutathione S-transferase enhances environmental adaptation of <i>Streptococcus mutans</i>]. [PDF]

Hua Xi Kou Qiang Yi Xue Za Zhi
Zheng H, Peng X, Zou J.
europepmc   +1 more source

[Influence of porosity and Young's modulus of gelatin methacrylate anhydride hydrogels on the biological behavior of mouse bone marrow mesenchymal stem cells]. [PDF]

Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi
Kong Y, Tian F, Liu QH, Zhang C, Song W, Kong Y, Li Z, Jirigala E, Huang S.   +8 more
europepmc   +1 more source

[<i>Poria cocos</i> polysaccharide alleviates cyclophosphamide-induced intestinal barrier dysfunction and inflammation in mice by modulating gut flora]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Zhang Y, Duan Y, Zhang C, Yu L, Liu Y, Xing L, Wang L, Yu N, Peng D, Chen W, Wang Y.   +10 more
europepmc   +1 more source