Zhongshan Daxue xuebao. Yixue kexue ban, 2005 【目的】分析前列腺特异性膜抗原(PSMA)基因片段在酿酒酵母中的诱导表达情况，以便进一步探讨重组人PSMA的免疫活性并完成抗前列腺癌PSMA蛋白疫苗的制备。【方法】应用基因重组技术将人PSMAcDNA序列克隆入酿酒酵母穿梭诱导表达载体pYES2／NT，构建重组酵母真核表达质粒pYES2／NT-PSMA，转化酿酒酵母菌株INVSc1，尿嘧啶筛选出阳性克隆转化子，经半乳糖诱导表达后进行菌体全蛋白的Western blot检测。【结果】成功构建了PSMA的酵母真核表达重组子pYES2／NT-PSMA ...
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