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前列腺特异性膜抗原基因片段在酿酒酵母中的诱导表达及意义

Zhongshan Daxue xuebao. Yixue kexue ban, 2005
【目的】分析前列腺特异性膜抗原(PSMA)基因片段在酿酒酵母中的诱导表达情况，以便进一步探讨重组人PSMA的免疫活性并完成抗前列腺癌PSMA蛋白疫苗的制备。【方法】应用基因重组技术将人PSMAcDNA序列克隆入酿酒酵母穿梭诱导表达载体pYES2／NT，构建重组酵母真核表达质粒pYES2／NT-PSMA，转化酿酒酵母菌株INVSc1，尿嘧啶筛选出阳性克隆转化子，经半乳糖诱导表达后进行菌体全蛋白的Western blot检测。【结果】成功构建了PSMA的酵母真核表达重组子pYES2／NT-PSMA ...
陈贤憬, 丘少鹏, 毛晓鹏, 王杜渐, 黎明涛   +4 more
doaj  

[Diffusion cycle-consistent generative adversarial networks for pelvic active bone marrow segmentation]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Zhuo L, Zeng M, Tan S, Liang T, Xiao W, Zhen X.   +5 more
europepmc   +1 more source

[Transcriptomic characteristics of cervical endplate cartilage in cervical spondylosis and effects of acupotomy on the FGF18/Akt axis with cervical spondylosis]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Liu F, Khaliunaa T, Cao Q, Yang Y, Ren C, Zhu J, Zhao X, Cao L, Deng B, Wang X.   +9 more
europepmc   +1 more source

[Efficient separation of gossypol from cotton kernels with copper mediated magnetic molecularly imprinted polymer]. [PDF]

Se Pu
Yang SL, Cao Y, He KL, Feng S, Zhang CG, Shan LH.   +5 more
europepmc   +1 more source

[Pathological characteristics of pigmented pretibial patches and vascular-immune abnormalities in diabetic patients]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Liu X, Chang B.
europepmc   +1 more source

[He<b>'</b>s Yangchao recipe ameliorates premature ovarian insuf-ficiency by remodeling intestinal microbiota to promote granulosa cell glycolysis]. [PDF]

Zhejiang Da Xue Xue Bao Yi Xue Ban
Lin F, Liu Q, Zhao Y, Chen Y, Wang R, Miao C, Zhang Q.   +6 more
europepmc   +1 more source

[Knockdown of <i>Cav1</i> inhibits mitochondrial function and mRNA m⁶A modification and expression of key genes in mouse hepatocytes]. [PDF]

Nan Fang Yi Ke Da Xue Xue Bao
Ding S, Liao Y, Bai X, Huang J, Asakawa T.   +4 more
europepmc   +1 more source

[Progresses on temporal interference electromagnetic stimulation for non-invasive deep brain function modulation]. [PDF]

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
Zhou T, Yu P, Xu Y.
europepmc   +1 more source

[Endometrial cancer lesion region segmentation based on large kernel convolution and combined attention]. [PDF]

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
Peng R, Zeng Q, He B, Liu J, Xiao Z.
europepmc   +1 more source

[Granuloma faciale and Takayasu arteritis in a child: a case report]. [PDF]

Zhongguo Dang Dai Er Ke Za Zhi
Liao W, Long J, Tang JP, Wo DN, Shu Y, Wei Z.   +5 more
europepmc   +1 more source